成骨细胞的

During a special biomineralization process—shell regeneration, we found that both CaLP exhibit special functions in shell related secretory vesicles; during the differentiation of osteoblast MC3T3-E1, CaLP exhibite significant effect on its differentiation and biomineralization.

在对切壳修复这一特殊的生物矿化过程的研究中发现CaLP积极参与了新生贝壳的形成；在前成骨细胞MC3T3-E1向成骨细胞分化的过程中，CaLP能够促进成骨细胞的分化和矿化过程。

Ore-binding factor alpha 1 plays an essential role in bone formation and osteoblast differentiation and serves as a master gene regulating osteoblast-specific gene expression. The gene is expressed in cells of the osteoblast lineage only, and when expressed in non-skeletal cells, the cells assume many of the characteristics of an osteoblast.

bfal在骨形成和成骨细胞分化过程中起着重要的作用，是调控成骨细胞特异性基因表达的主控基因，该基因只在骨形成相关细胞中表达，如果使其在非成骨细胞中表达，该细胞将出现成骨细胞的特征。

Methods The embryo calvarial periosteum tissue was taken and, human osteoblasts were obtained by enzyme-assimilating methods. The morphological change, growth feature and osteogentic capability of osteoblasts were observel during culture in vitro, drew the growth curve was graphed and the cells was identified by alkaline phosphatase dye. At the same time, the morphology and bioactivity of 3 to 5 th-generation osbeoblasts and anabiotic cells were studied comparatively.

取人胚胎颅骨骨膜，采用酶消化法获取成骨细胞体外培养并传代，观察细胞形态，生物特性，绘制生长曲线，并经碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3~5代与冻存后成骨细胞的特点。

The authers fetched the embryo calvarial peristeum tissue, got human osteoblast by enzyme-assimilating methods and tissue-block culture methods. We observed the morphological change, growth feature and osteogentic capability, of osteoblast during culture in vitro with phase contrast invert microscope, drew the growth curre and identified the cells by alkaline phosphatase dye. At same time, the morphology and bioactivity of 3-5th-generation osbeoblast and anabiotic cells was studied comparatively. 2. titanium particles were examined by scanning electron and the size was determined by semi-automated image analysis. The 3-5 th gereration of human osteoblast were cultured in medium with different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml). Cell growth and proliferation was detected by MTT method after 2、4、6 days that particles were added into medium and ALP activity was measured by kit after 4、7、10 days respectively. 3. With above same methods,the 3-5th generation of human osteoblasts were cultured for 3、6、9days after different concentration of particulates titanium alloy (1mg/ml, 0.1mg/ml, 0.01mg/ml) were added into the medium and OPG gene expression was quantified by RT-PCR.

1、取人胚胎颅骨骨膜，采有用酶消化法和组织培养法获取成骨细胞体外培养并传代，观察细胞形态，生物特点及成骨特性，并绘制生长曲线同时碱性磷酸酶染色鉴定成骨细胞以及比较冻存前3-5 代与冻存后成骨细胞的特点。2、电镜下观察钛合金颗粒的形态并测量其粒径，将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞共同培养，分别于第2、4、6 天用MTT 法测量细胞增殖情况及4、7、10天用试剂盒检测碱性磷酸酶活性。3、分别将不同浓度的钛合金颗粒(1mg/ml，0.1mg/ml，0.01mg/ml)与成骨细胞基因培养3、6、9 天用RT-PCR 方法半定量测定骨保护素基因mRNA 的表达。

Identification of these cells: Over 90% cells isolated from human fetal calvaria were stained positively for both alkaline phosphatase and Alizarin red.

采用酶消化后植块的改良植块培养法可在较短时间内获得大量成骨细胞，这些细胞具有典型的成骨细胞形态和功能，是一种较好的分离培养人胚成骨细胞的方法。

Results:(1) CBC possesses a natural trabecular structure. Its components is similar to the mineral of bone.(2) CBC has great cellur biocompatibility. It can promote cell adhesion, proliferation and secretory while needn t modification.(3) In nude mice, the ectopic osteogenesis of MCCAB was obvious which was better than that of CBC alone and the new bone formation increased in pace with time. In the MCCAB, both intramembranous and cartilaginous osteogeneses were seen but the former dominated.(4) When MCCAB was autotransplanted intramuscularly, the osteogenesis was much better than that of negative control groups.(5) The MCCAB can repair autogenous calvaria defects effectively and the effect was much better than that of CBM alone and blank group. Conclusion:(1) CBC possesses good biocompatibility, osteoconduction and porous structure, can be used as scaffold material for constructing of tissue engineering bone.

结果：(1)CBC具有天然的多孔网状结构，最大程度地保持了骨矿的微结构特征，可以作为骨组织工程的支架材料；(2)CBC细胞亲和性好，不需作修饰即能促进成骨细胞的粘附、增殖和分泌活动；(3)MSO/CBC复合人工骨在裸鼠皮下异位成骨作用明显，兼有膜内成骨和软骨内成骨的特点，成骨效果明显优于单纯CBC植入组；(4)MSO/CBC复合人工骨兔自体肌袋异位成骨作用明显，血管化充分，成骨效果明显优于单纯CBC植入组；(5)MSO/CBC复合人工骨修复兔颅骨临界性缺损效果好，明显优于单纯植入CBC组和空白对第四旱区大学硕士学位论文一照组，可以作为骨移植替代材料用于骨缺损畸形修复。

In addition of cytomorphologic identification, immunolhistochemic technique of bone morphogenic protein-monoclonal antibody, alkaline Phosphatase staining and concentration assays, as well as osteoblasts reaction to PTH-cAMP were used, according to the biologic character of osteoblasts.

除了在细胞形态学上对其鉴定外，还根据成骨细胞的生物学特性，首次引用了骨形成蛋白单克隆抗体免疫细胞化学技术，以及碱性磷酸酶染色和含量测定，成骨细胞对甲状旁腺激素-cAMP反应等方法，验证了经酶消化分离培养的兔颅骨细胞具有成骨细胞的功能和特性。

The osteogenous ability on the surface of UMCHA: MC3T3-E1 osteoblast was cultured on the surface of UMCHA to observe early stage of adhesion and proliferation, the osteogenous ability and configuration, BCBC and blank groups being controls.

UMCHA表面培养成骨细胞的活性：在UMCHA表面培养MC3T3-E1成骨细胞，并以空白和BCBC作为对照，观察成骨细胞的粘附率、增殖率、成骨活性和细胞形态。

Effects of sinusoidally varying magnetic fields with one frequency of 2HZ and static magnetic fields with different strength of 10GS、200GS and 560GS on proliferation of osteoblasts had been observed. It had been found that PEMFs promoted proliferation of osteoblasts, static magnetic fields with intensities of 10GS and 200GS did not affect its proliferation whereas that of 560GS inhibited the proliferation.

通过观察频率为2HZ的正弦波电磁场及强度分别为10GS、200GS、560GS的静磁场对成骨细胞增殖的影响，结果显示：脉冲电磁场能显著促进成骨细胞的增殖；强度为10GS、200GS的静磁场对成骨细胞的增殖无明显影响，而强度为560GS的静磁场抑制成骨细胞的增殖。

Studying the method of culturing the osteoblast cell in vitro: By digestion with enzyme and tissue explanting, cranial bone of neonatal SD rat was

极低频矩形波电磁场对成骨细胞增殖与分化的影响采用不同强度、频率和占空比的REMF作用于成骨细胞，检测成骨细胞的

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐