成骨的

Recombinant Human Osteogenic Protein (rhOP-1) provides a strong arm to solve this difficult problem. The rhOP-1 is produced by DNA recombinant technique, with the characteristics of high purity, high output, low cost and high osteoinductive activity. How does OP-1 work on the erective spinal fusion?It has not been documented yet.

重组人OP-1（rhOP-1）的出现为这个问题的解决提供了一个强有力的武器。rhOP-1是通过DNA重组技术生产，具有纯度高、产量高、成本低等优点，具有高效的诱导成骨活性。rhOP-1在直立脊柱融合术中的作用如何，目前尚未见文献报道。

Although underlying mechanisms ⑴ hae not been elucidated, eidence points to the participation of the nitric oxide and cyclic guanosine 3',5'-monophosphate system.

虽然根本的机制还没有阐明，但是证据显示一氧化氮和环鸟苷酸系统参与其中。这里，我们检测到在MC3T3-E1成骨细胞的分化中基质金属蛋白酶13的mRNA，蛋白和活性的升高，诱生一氧化氮合酶和一氧化氮产物的升高。

The changes have important effect on the stability of the treatment and the function of mastication.

通过牵引成骨和正畸联合矫治严重上颌后缩后，上颌骨的前移和牙齿的移动必然使上下牙的排列和咬合发生变化，而这种变化对疗效的稳定性和患者的咀嚼功能的恢复有着重要影响。

We carried out a primary study of shear stress activated K+ channel in osteoblast by using clamp patch technology .

研究原癌基因表达与剪应力的关系对于了解细胞通过何种途径将剪应力信号的刺激传入细胞内并引起一系列的细胞应答，对于进一步认识流动剪应力作用对成骨细胞功能、代谢等产生的影响都有重要的意义。

The effect of bavachin and corylin, two active principles from Psoralea corylifolia L., on UMR106 cyclin E expression by Western blotting was examined. The result showed that the two flavonoid compounds had no significant effect on cyclin E expression after various exposure time.

对于中药补骨脂中分离的两个促成骨样细胞增殖的活性成分补骨脂二氢黄酮和补骨脂异黄酮，采用Western blotting的方法考察不同时间对UMR106细胞周期蛋白cyclin E的表达，结果它们对cyclinE的表达没有显著影响。

In the part 3 of the thesis, we develep the animal model using the method of part 2 of the thesis. 6weeks later, Clearance of focal lesion in necrosis femoral head is performed. Cancellous bone adding rhBMP〓 is implanted in the space after sequestrum cleaning. Imagical、morphology and biomechanics study show its result is superior to traditional cancellous bone grafting.

因此，在论文的第三部分，我们首先按照本论文的第二部分的方法，制作股骨头坏死的动物模型，6周后，行坏死股骨头病灶清除术，分别采用将松质骨、〓+松质骨屑植入死骨清除后空腔内，通过影像学、形态学及生物力学研究显示，〓+松质骨组的成骨速度及质量优于传统的松质骨移植。

Although Albee also described the use of calcium phosphates as an alternative to bone in the 1920s, it was not until 1965, when Marshall Urist identified "bone formation by autoinduction"2, that new options unfolded.

尽管Albee也曾在1920年代提出过使用硫酸钙作为骨的替代品，但直到1965年Marshall Urist确定了&自身诱导的成骨&，新的选择展现在了我们的面前。23年后Wozney小组及Luyten小组发现了引起这一现象的蛋白BMPs-2、 3和4。

Although Albee also described the use of calcium phosphates as an alternatie to bone in the 1920s, it was not until 1965, when Marshall Urist identified "bone formation by autoinduction"2, that new options unfolded.

尽管Albee也曾在1920年代提出过使用硫酸钙作为骨的替代品，但直到1965年Marshall Urist确定了&自身诱导的成骨&，新的选择展现在了我们的面前。23年后Wozney小组及Luyten小组发现了引起这一现象的蛋白BMPs-2、 3和4。

The results show that the hydrophilicity of PLA film is improved by introduction amido: the contact angle decreases from 78° to 56° and the free energy increases from 42.7mJ/m^2 to 51.4mJ/m^2. Compared with the control, osteoblast spread out more filopodia after 3 d incubation on amidation surface, and it entered cell division phase more quickly, which indicated that the surface amidation could promote the cell's adhesion and proliferation on the PLA film.

结果表明，酰胺基的引入改善了PLA膜的表面亲水性，其表面水接触角由78°减少到56°，自由能由42.7mJ/平方公尺增大到51.4mJ/平方公尺；与对照组相比，成骨细胞在改性表面培养3d后有大量的丝状伪足伸出，并且较快地进入了细胞分裂期，表明PLA膜表面的酰胺化能够促进细胞的黏附和增殖。

In resent year, several in vitro models for committed differentiation of ES cells have been described which give rise to a variety of cell types, including hematopoietic, neuronal, chondroitic, osteocytic, endothelial, myogenic, cardiac and smoothes muscle cells, but the committed differentiation into epidermal stem cells have not been reported.

ES细胞为研究基因功能、胚胎发生和个体发育，及组织细胞分化机理提供了理想的模型。近年来的研究表明，适当条件下，ES细胞可定向分化为三个胚层的各种类型的细胞，其中包括向神经元、造血细胞、成骨细胞、心肌细胞、血管内皮、软骨细胞、平滑肌特方向的定向分化。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。