成骨的

Methods Serum containing achyranthes root was prepared, human mesenchymal stem cells were separated and amplified, MTT assay was used to access the effects of serum containing achyranthes root on human mesenchymal stem cells in vitro, alkaline phosphatase and osteocalcin were assayed to reflect the variations of stem cell differentiation, and the expression of osteogenic transcription factors with RT-PCR.

制备牛膝含药血清，分离与扩增人间充质干细胞，MTT法检测牛膝含药血清对体外培养的间充质干细胞增殖作用的影响，检测碱性磷酸酶与骨钙素以反映细胞分化水平的改变，RT-PCR检测成骨相关转录因子的表达。

Then, under the control of systemic factors, mesenchymal cells aggregate and differentiate to form catilage blastemal elements that prefigure skeletal limb components.

脊椎动物胚胎期骨与关节系统的发生是一种复杂生命现象，起始于中胚层间充质细胞的定向聚集，形成肢芽，然后在一系列作用因子的调控下，肢芽内细胞进一步分化，形成具有骨骼雏形的软骨原基，后者经软骨内骨化发育成骨。

Methods: 12 fresh frozen adult leg-foot specimens were mounted in a coordinated system, a Steinmann pin was perforated across the calcaneus, cuboidcum, talus, and naviculare as markers. The displacements of the marked points in the coordinated system were recorded. Through matrix translation and Euler equation, the rotational angle of the calcaneus, cuboideum, talus, naviculare were calculated, which represented the motion of the calcaneocuboid, talonavicular and ankle joint in different condition respectively, while statistical analysis were also taken between different conditions.

取新鲜足标本12例，通过加载使足产生某种形式的运动，用三维数字化坐标仪测量距下关节组成骨在某种运动状态下的相对三维坐标位移，通过矩阵转换和求解非线性函数方程计算其三维旋转角度，确定距下关节的运动范围、规律和在足整体运动中的作用。

Here we investigate the effects of 17 β-estradiol (10〓～10〓mol/L) on the proliferation and differentiation of newborn rat calvaria osteoblastic cell.

对体外培养的成骨细胞施以不同剂量的17β-雌二醇，3天后通过细胞计数、〓—胸苷掺合、细胞定量后测定骨钙素及碱性磷酸酶的量观察对增殖和分化的影响。

Up-regulating expression of VEGF and FGF-2 at molecular level and promoting preosteoblast and chondroblast caryocinesia ,bloodvessel growth and osseous anabolism.

节VEGF和FGF-2的表达，促进前成骨细胞和成软骨细胞的有丝分裂、血管内生以及骨的合成代谢达到促骨折愈合的作用。

The fundamental imaging features of osteosarcoma were showed bone destruction, periosteal reaction, neoplastic bone and soft-tissue mass. Among 49 cases examined by radiography, osteoblastic pattern sarcoma was found in 19 cases, osteolytic pattern sarcoma was found in 13 cases and mixed pattern sarcoma was found in 17 cases. The detection rates of bone destruction, periosteal reaction, soft-tissue mass, neoplastic bone and Codman triangle by radiography were respectively 61.2%, 95.9%, 71.4%, 73.5%and 27.7%. All 10 cases with CT scan were showed bone destruction, soft - tissue mass and neoplastic bone. The detection rates of periosteal reaction,憇ieve sign挶 and Codman triangle with CT scan were respectively 90%, 90% and 11%. The tiny neoplastic bone and"sieve sign"were showed sensitively by CT, but Codman triangle was showed by radiography superior to CT.32 cases with MRI scan presented the normal marrow was replaced by the tumor, which was showed low to intermediate signal intensity on T1-weighted images and promiscuity signal intensity on T2-weighted images. 7 cases presented hemorrhage and 24 cases presented necrosis.

骨肉瘤基本影像表现为骨质破坏、骨膜反应、瘤骨和软组织肿块。49例X线表现为成骨型19例，溶骨型13例，混合型17例；X线对骨质破坏、骨膜反应、软组织肿块、瘤骨和Codman三角的检出率分别为61.2%、95.9%、71.4%、73.5%和27.7%；10例CT表现均可见骨质破坏、软组织肿块及瘤骨，CT对骨膜反应、筛孔征和Codman三角的检出率为90%、90%和11%；CT对显示细小肿瘤骨和筛孔征最敏感，但对Codman三角的显示不如X线平片；32例MRI表现为骨髓腔内正常的骨髓高信号被肿瘤信号所取代，T1WI呈低～等信号，T2WI呈混杂信号，7例合并出血，24例中央坏死囊变，32例增强扫描均呈不均匀强化及瘤周可见水肿区。

From the theoretical angle, to analyze and summarize the morbility reason of Senile Osteoporosis, to approach the effect of the flavone of rhizome drynaria on the lipidision of marrow stroma cell, as well as the senescence and apoptosis of osteoblast through the experimental study; from the clinical angle, to observe the clinical effect of the flavone of rhizome drynania on Senile Osteoporosis, to supply scientific theory basis for clinical application.

从理论角度分析总结老年性骨质疏松症的发病原因，并通过实验研究探讨骨碎补总黄酮对骨髓基质细胞脂质化的影响，以及对成骨细胞衰变和凋亡的影响，从临床角度观察其治疗老年性骨质疏松症的效果，为临床应用提供科学理论依据。

Objective To investigate whether osteoblasts cultured in vitro can form mineralization nodes. Method The osteoblasts at 1×105/ml,2×105/ml and 5×105 /ml cell density were cultured in the DMEM medium and in the DMEM medium supplemented with β-glycerophosphate sodium and ascorbic acid.

目的 了解体外培养成骨细胞的接量对钙化结节形成的影响方法实验选用了1×105 /ml、2×105个/ml、5×105个/ml三个不同的细胞接种浓度，并且每个浓度组分别给予含β-甘油磷酸钠和维生素C的条件培养液以及常规培养液进行细胞培养。

The biochemistry and image analyzing were used to investigate the effects of the PDGF-AA at high concentration on the osteoclastic bone resorption in the osteoblast-osteoclast co-culture system.

在高浓度PDGF-AA的作用下，应用生化检查和图象分析技术，检测PDGF-AA对成骨细胞-破骨细胞共育体系中破骨细胞骨吸收功能的影响；应用免疫荧光检测破骨细胞合成抗酒石酸酸性磷酸酶的变化；应用免疫组化和RT-PCR技术检测骨保护素配体的表达。

Osteogenic differentiation of MSCs in the hybrid scaffold was greatly inuenced by the addition of growth factors.

这篇文章的主要目的是评估兔的MSCs在一种含有HA的三维凝胶支架中的异位成骨情况。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。