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In order to observe the capacity of composite artificial bone made from collage/Nano tricalcium phosphate conducting subperiosteal bone formation and investigate the possibility applied in reconstruction of astrophy aveolar ridge, the composite artificial bone was implanted into subperiosteum of the cranial bone in rabbits.

将胶原与纳米磷酸三钙复合制成复合人工骨并植入兔颅骨的骨膜下作为实验组,使用显微镜测微尺测量不同时间点新骨自骨表面长入材料内的高度,并和羟基磷灰石组作对照,观察 Co/N- TCP复合人工骨引导成骨能力,探讨其用于萎缩性牙槽嵴重建的可行性。

The proliferation effect of eight extracts of four Urtica plants on clone MC3T3-E1 cells was examined with cell counting kit-8 (CCK-8) and alkaline phosphatase activity was also determined.

结果三角叶荨麻根的醇提物,宽叶荨麻全草的醇提物和醇提物水层过D101大孔树脂40%洗脱部分在50到200 μg/ml 的浓度下能显著促进成骨样细胞MC3T3-E1增殖。

The proliferation effect of eight extracts of four Urtica plants on clone MC3T3-E1 cells was examined with cell counting kit-8 (CCK-8) and alkaline phosphatase activity was also determined.

结果三角叶荨麻根的醇提物,宽叶荨麻全*的醇提物和醇提物水层过D101大孔树脂40%洗脱部分在50到200 μg/ml 的浓度下能显著促进成骨样细胞MC3T3-E1增殖。

Osteoblasts isolated from the cranium of Zelanian rabbit,after being transfected with green fluorescent protein,were inoculated onto the bio-derived bone to investigate the 3D fabrication of engineering bone in rotating wall vessel bioreactor.

用经绿色荧光蛋白标记的新西兰兔颅骨来源的成骨细胞复合到生物衍生骨支架材料上,在新型旋转壁式生物反应器内三维构建组织工程骨,同时与静态培养环境中构建的结果进行比较。

Objective:To explore the application of the basic fibroblast growth factor combined partially demineralized allogenic bone in mandibular defects, and to study the effects of bFGF in bone formation as well as the selection of its carrier.

目的:探讨bFGF(basic fibroblast growth factor ,碱性成纤维细胞生长因子)复合部分脱矿冻干辐照异体骨在修复下颌骨缺损中的应用,研究bFGF在骨缺损修复中的成骨作用机制及其适宜载体的选择。

And that single calcium phosphate artificial bone carrier material all have their own defects in these aspects.

学强度、良好的组织相容性、可生物降解吸收、载药和缓释性能以及成骨活性等,而单一磷酸钙类人工骨载体材料在这些方面郡有看各自不同的缺陷,而把多种材料进行组合优化,是研究的热点。

Objective To study the effects of fluoride on expression of calmodulin in rat osteoblasts cultured in vitro in different doses and at different time.

目的 观察不同浓度的氟在不同时间段对体外培养大鼠成骨细胞的钙调蛋白的影响。

METHODS: Firstly,depanding on the methods of FCM,MTT etc,we got the best density and actiontime of adriamycin.and then,we used several depressors of different signal transduction pathways to observe the possible pathway of osteosarcoma cell apoptosis.

首先通过FCM,MTT等方法探讨引起成骨肉瘤细胞凋亡的最佳药物作用浓度及时间;在此条件下应用不同信号转导通路的抑制剂观察导致细胞凋亡的可能通路;最后应用Western????

Objective To investigate the dose-effect effects of th ree relating bone growth factors, dexamethasone, recombinant human basic fibro blastic growth factor and recombinant human bone morphogenetic protein- 2 (rhBMP-2), on proliferation and differentiation of periosteal cells so as to provide experimental basis for their further application in bone tissue engineer ing.

骨膜细胞是骨修复、重建过程中重要的功能细胞,在一定条件下具备向成骨细胞分化,分泌骨细胞外基质的潜能,因此成为骨组织工程研究中构建细胞-材料复合体非常重要的种子细胞。

The present paper is to study the effect of insulin on the biological activity of osteoblasts obtained from diabetic rats mandibles,and to provide feasibility evidence for the local application of insulin via jaw bone.

本文将研究胰岛素对体外培养的糖尿病大鼠下颌骨成骨细胞生物学活性的影响,为胰岛素的局部植入式给药提供理论依据。

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