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The product contains recombinant human bone morphogenic protein in a collagen scaffold. The protein promotes differentiation of cells into bone-producing osteoblasts when implanted into target areas of the spine.

该产品为一胶原支架,内含重组的人类促骨生成蛋白;当移植入脊椎的目标区时,此蛋白可以促进细胞分化至制造骨质的成骨细胞内。

Results High homogenous hMSCs were obtained and after co-culture for 5 d with osteoblasts, they fused to form multinuclear cell clusters in micrograph and were postive in TRAP staining. Conclusion The co-culture system method is successful.

结果:体外分离、培养的hMSCs具有独特的细胞免疫学表型,即CD73和CD105阳性, CD34和CD45 阴性。hMSCs与成骨细胞共同培养5 d后,即可见单个核细胞融合成多核细胞,TRAP 染色可见多数细胞胞浆呈酒红色,为诱导成功的破骨细胞。

Results:The effect of P18 on effect on the proliferation on osteoblastic cell depends on the condition of cell culture.

P18对于成骨细胞的作用与TGF-β类似,由于其还具有HA结合活性,在HA骨修复材料的改进以及种植体涂层修饰方面有着广阔的应用前景。

BMP, TGF-β and bFGF possessed their own different characteristics of expression and distribution and cooperatively modulated proliferation of osteogenetic cells and differentiation of chondrocytes and osteoblasts for fracture healing.

BMP、TGF-β和bFGF有着各自的表达和分布特点,并共同调解骨原细胞的增殖和成骨细胞、软骨细胞的分化,最终完成骨折修复。

Methods Osteogenetic culture medium containing different concentrations of atorvastatin 10^(-6, 10^(-7), 10^(-8)mol/L and control were added in the murine bone marrow cell subculture. The proliferation of the stromal cells was observed, and alkaline phosphatase staining and examination were done to check the differentiation ability of bone marrow cells. Alizarin red staining was performed to evaluate the mineralization of the osteoblasts in culture.

分别将10^(-8)、10^(-7)、10^(-6)mol/L的阿托伐他汀和空白药物加入传代培养的小鼠骨髓基质细胞,通过细胞形态学观察、细胞增殖率检测、碱性磷酸酶检测和钙结节染色,比较药物在成骨细胞分化过程中对细胞增殖和分化的影响。

Simvastatin leads to the high expression of cbfa1 in bone marrow stromal cells and increased ALP activity, which may be parts of the mechanisms underlying the anabolic osteogenetic effect of simvastatin.

本研究采用体内给药然后体外对骨髓基质细胞培养诱导的方法,旨在观察辛伐他汀对骨髓基质细胞成骨分化的影响,以探讨其促进骨形成,治疗骨质疏松的机制。

RESULTS AND CONCLUSION: Compared to normal oxygen, hypoxia promoted the proliferation and osteogenic differentiation of MSCs.

研究表明,低氧促进MSCs的增殖[8-13],但是在研究低氧对MSCs成骨分化的影响时有不同的结论。

To investigate a new method of preparing the PCHA bone cement, and to test its physicochemical properties, and to evaluate its degrading, osteogenous ability and compressive strength.

目的 探讨原位固化制备多孔生物材料的新方法;制备多孔碳酸化羟基磷灰石骨水泥;完成PCHA骨水泥的理化性能检测;验证PCHA的降解性能、成骨活性和强度变化。

Spindle shape of cells gradually disappeared, with increased cell body. Some cells grew in cluster. MSCs changed figures to multilayer knots 10 days after inducing osteoplastic differentiation.

骨髓间充质干细胞向成骨分化过程中,纺锤形的突起逐渐消失,胞体增大,培养10 d后,部分细胞呈聚集生长,随细胞生长密度的增长形成多层的结节结构。

BACKGROUND: Osteoblast allograft has been considered as an ideal wayto enhance the rehabilitation of porotic bone due to its particular property.

背景:成骨细胞的同种异体移植,因其独特的优点,被认为是解决衰老骨重建修复较为理想的方法。

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