成骨的

And cell differentiation was assayed by measuring the cellular alkaline phosphatase activity with dexamethasone as positive control. The influence of ethanol and dimethyl sulfoxide on cell proliferation and differentiation assay was also investigated.

成骨样细胞的分化采用测定细胞内碱性磷酸酶活性的方法，地塞米松为阳性对照药，同时考察了溶剂乙醇和二甲基亚砜对细胞增殖和分化的影响。

In order to avoid prolin residue inhibiting enterokinase cleavage, 9bp of MGF cDNA 5′ end sequence was truncated by primer, then the obtained truncated MGF des(1-3MGF cDNA (321 bp) was subcloned in pET32a vector to construct a prokaryotic recombination expression plasmid.

通过RT-PCR从拉伸刺激的人成骨细胞中克隆MGF cDNA序列，并去除5'端9 bp的序列，使N端缺少对肠激酶(Enterokinase， EK)具有抑制作用的脯氨酸，将截短型MGF des(1-3 MGF cDNA序列克隆入pET32a质粒，构建重组表达质粒。

More clones expressed phenotypes of osteoblasts (48%), chondrocytes (43%), and neuron-like cells (52%) than of adipocytes (12%), possibly due to the loss of adipogenic ability after repeated subcultures.

多数克隆的表型是成骨细胞（48%）、软骨细胞（43%）、神经元样细胞（52%），而脂肪细胞表型的克隆仅12%，这可能由于多次传代培养后成脂能力的丧失。

However, by removing the effects of proliferation from the experiment, AMSCs no longer out-performed BMSCs in their ability to undergo osteogenic and adipogenic differentiation.

然而，在实验中排除细胞的增殖作用，AMSC没有比BMSC的成骨和成脂的分化能力更强。

METHODS: From January 2000 to May 2002, 52 cases with various types of bone defect were treated with tissue engineered bone, which was constructed in vitro by allogeneous osteoblasts from periosteum 1 x 10(6/ml with bio-derived bone scaffold following 3 to 7 days co-culture. Among them, there were 7 cases of bone cyst, 22 cases of non-union or malunion of old fracture, 15 cases of fresh comminuted fracture of bone defect, 4 cases of spinal fracture and posterior route spinal fusion, 3 cases of bone implant of alveolar bone, 1 case of fusion of tarsotarsal joint.

2000年1月～ 2002年1月，用同种异体骨膜来源的成骨细胞 1×106 / ml与生物衍生骨支架材料构建的组织工程骨，经体外培养 3～ 7天后，植入修复52例各种类型的骨缺损，其中骨囊性病变7例，陈旧性骨折不愈合或畸形愈合 2 2例，新鲜粉碎性骨折伴骨缺损 15例，脊柱骨折后路植骨融合术4例，牙槽骨植骨 3例，足跗中关节植骨融合 1例。

Calcified rheumatic valve neoangiogenesis is associated with vascular endothelial growth factor expression and osteoblast-like bone formation.

钙化的风湿性瓣膜的血管新生与血管内皮生长因子的表达和成骨样细胞骨形成相关

To investigate the effect of Jiangu granula on the apoptosis of newborn rat calvaria osteoblast induced by TNF-ɑ in vitro.

目的 探讨健骨颗粒含药血清对TNF-ɑ体外诱导的新生大鼠成骨细胞凋亡的影响；进一步揭示健骨颗粒改善骨质疏松模型鼠骨量的可能机制。

Objective: To investigate the effects of recombinant human insulin-like growth factor on the proliferation , apoptosis and anabolic function of cultured rat osteoblasts in vitro.Method: Rat calvaria cells were cultured in medium with or without rhIGF-I.

目的 通过观察重组人胰岛素样生长因子I对体外培养的大鼠成骨细胞增殖、凋亡和功能的影响，探讨rhIGF-I对骨代谢影响的可能机制。

The results showed that:a Cyclic strtching at 500με,1000μεand 1500μεwith the frequency of 0.4Hz affected the physiological activity of osteoblasts delivered from rat calvaria.

应变分别为500με、1000με和1500με，频率为0.4Hz的周期性拉伸刺激影响大鼠颅盖骨中分离出的成骨细胞的生理活性。

The effects of situ hybirdization study show that the expression of NO-i increases evidently in the cytolymph of bone. It proves that GSL can obviously increase the content of NO-i and promote the synthesis of bone and secretion of iNOS.

组化及原位杂交研究结果显示，在软骨细胞的胞浆中，NO合成酶的表达显著增加，表明骨疏灵能明显增加NO合成酶的含量，并能促进成骨细胞合成和分泌NO合成酶。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。