成骨的

Human BMSCs after being induced have the strong osteogenic ability, and the compounds of decell decalcification spongy bone human BMSCs osteoblast is a kind of ideal ossification of tissue engineering.

人BMSCs诱导的成骨细胞具有良好的体内成骨能力，脱细胞脱钙骨松质人BMSCs 成骨细胞是较理想的组织工程化骨。

In conclusion ,the results of this study showed that the inducing agent HMBA could effectively inhibit the proliferation activity of the human ostosarcoma MG-63 cells,cause the arrest of G0/G1 phase, reverse the malignant phenotype characters,upregulate expression of the osteoblat-like phenotype markers including collagen,osteocalcin,osteonectin , facilitated the formation of bone nodule .

本研究的结果表明HMBA能够有效抑制人成骨肉瘤MG-63细胞增殖活动，改变肿瘤增殖核抗原表达，将细胞周期阻滞在G0/G1期，逆转人成骨肉瘤细胞恶性表型特征，在促进终末分化标志物Ⅰ型胶原纤维蛋白、骨钙蛋白、骨粘素的表达同时，促进骨结节生成，从而说明HMBA能够诱导人成骨肉瘤MG-63细胞终末分化，并且Rg1、BPO-L、Rg1+BPO-L以及Rg1+HMBA等均具有终末诱导分化能力。

Methods: Human osteoblasts were cultured on flexible-bottomed plates and subjected to 8% elongation by stress unit at 6 cycles/min (i.e. 5-s elongation and 5-s relaxation) for 24 hours in the experimental groups. Control group did not exert any stress.

通过体外细胞加载系统对培养的人成骨细胞施加8%形变率、6周／min的周期性牵张力24 h，应用基因表达谱芯片技术对人成骨细胞中mRNA表达水平进行对比杂交分析，检测周期性牵张力加载组成骨细胞基因表达谱的变化。

In SMC group, relative stable of the both ends of fracture reduce unnessary repeated stimulation of injury, and at same time, axio-stress advantageous for preosteoblast and mesenchymal cell transmit to osteoblast and chondroblast. Material and design superiority of SMC form unique internal mechanical environment, and which benefit for bone healing. The study took deep investigation of SMC on fracture healing.

上述结果证实：SMC内固定可对骨折端施加主动的、持续的轴向应力刺激，形成沿长骨骨干轴向的成骨导向力值，配合环抱翼的把持作用，形成了区别于CP、IN的独特的稳定、有效的机械内环境，骨折端的相对稳定减少了不必要的损伤再刺激，同时持续轴向应力有利于成骨祖细胞、间充质细胞向成骨细胞和成软骨细胞转化、促进了成骨诱导过程的发生与发展，加快了骨折愈合的修复与重建。

Results We can induce MSCs into osteoblasts which can express gene col Ⅰ and ALP. Through analysis growth curve, we demostrateed the diphosphonate or assemble flavone of drynaria rhizome with 10^(-4)~10^(-6)mmolL^(-1) had the inhibition on the osteomast; it with 10^(-8)mmolL^(-1) had a promotion on the osteoblast; it with 10^(-10)~10^(-12)mmolL^(-1) had a restraint on osteoblast. Conclusion Through gene analysis, we found the diphosphonate or assemble flavone of drynaria rhizome can have the promotion on the osteoblast.

结果 骨髓基质细胞经过4周诱导可转化为表达colⅠ和ALP的成骨细胞，经过生长曲线分析，二磷酸盐和骨碎补总黄酮10^(-4)~10^(-6)mmolL^(-1)时，对诱导后的成骨细胞有抑制作用；10^(-8)molL^(-1)有促进作用；10^(-10)~10^(-12)mmolL^(-1)不明显，其中10^(-8)mmolL^(-1)作用最强；基因分析后，发现二磷酸盐和骨碎补总黄酮促进成骨细胞表达colⅠ和ALP，且两者合用效果大于单独用药。

The procedure could provide firm internal fixation,improve activity of osteoblasts in fractural sites.accelerate fracture healing.

上述方法可提供早期坚强的内固定，补充成骨基质和成骨前质细胞，改善成骨能力。

Microscope examination:in group A,at 2 weeks,a few inflamatory cells appeared in the holes with many osteoblasts and ostoid appeared around the drilled holes.At 8 weeks,marrow formed in the drilled holes in group B,at 2 weeks,there were a large amount of osteoblasts in the drilled holes and some ostoid formed around thc drilled holes.At 4 weeks,the drilled holes were full of trabeculae.At 8 weeks,the trabeculae matured with the marrow appeared in the intertrabecular space.

组织学结果：2周时A组钻孔区出现少许炎症细胞，边缘出现较多成骨细胞并有骨组织形成，至8周时，钻孔区内形成骨髓组织，只在边缘形成骨小梁结构。2周时B组钻孔区有大量的成骨细胞，边缘有较多骨组织形成，4周时钻孔区内充满重生骨小梁结构，8周时钻孔区内骨小梁成熟，小梁有骨髓组织填充。

Microscope examination:in group a,at 2 weeks,a few inflamatory cells appeared in the holes with many osteoblasts and ostoid appeared around the drilled holes.at 8 weeks,marrow formed in the drilled holes in group b,at 2 weeks,there were a large amount of osteoblasts in the drilled holes and some ostoid formed around thc drilled holes.at 4 weeks,the drilled holes were full of trabeculae.at 8 weeks,the trabeculae matured with the marrow appeared in the intertrabecular space.

组织学结果：2周时a组钻孔区出现少许炎症细胞，边缘出现较多成骨细胞并有骨组织形成，至8周时，钻孔区内形成骨髓组织，只在边缘形成骨小梁结构。2周时b组钻孔区有大量的成骨细胞，边缘有较多骨组织形成，4周时钻孔区内充满新生骨小梁结构，8周时钻孔区内骨小梁成熟，小梁有骨髓组织填充。［结论］骨髓基质干细胞对兔股骨头缺血性坏死有良好的修复作用。

The results indicated that the osteogenetic ability of the study group was significantly higher than that of the control group at the same time point, and the neogenetic bone increased along with the prolongation of the implantation time.

结果：实验组4周和8周移植物组织块ALP活性分别为(63.48±0.873)和(69.527±0.635)IU/L，移植物组织块成骨含量分析分别为(2.50±0.38)和(4.70±0.67)分，对照组4周和8周移植物组织块ALP活性分别为(2.50±0.38)和(4.70±0.67)IU/L，移植物组织块成骨含量分析分别为(1.90±0.54)和(3.40±0.54)分，表明，实验组在同时间点的成骨能力显著高于对照组，并随植入时间的延长，新骨形成增多。

Effects of sinusoidally varying magnetic fields with one frequency of 2HZ and static magnetic fields with different strength of 10GS、200GS and 560GS on proliferation of osteoblasts had been observed. It had been found that PEMFs promoted proliferation of osteoblasts, static magnetic fields with intensities of 10GS and 200GS did not affect its proliferation whereas that of 560GS inhibited the proliferation.

通过观察频率为2HZ的正弦波电磁场及强度分别为10GS、200GS、560GS的静磁场对成骨细胞增殖的影响，结果显示：脉冲电磁场能显著促进成骨细胞的增殖；强度为10GS、200GS的静磁场对成骨细胞的增殖无明显影响，而强度为560GS的静磁场抑制成骨细胞的增殖。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐