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OBJECTIVE: To introduce proteomics, to summarize the research of proteomics in directional differentiation of BMSCs, and to forecast the development of proteomics research methods.

目的:介绍蛋白质组学的概况,总结蛋白质组学在骨髓间充质干细胞向心肌、成骨及脂肪细胞定向分化方面的研究进展,讨论其面临的问题,并对蛋白质组学研究方法进行展望。

A stable and duplicable experimental dog model on study of vertical alveolar distraction osteogenesis have established successfully by the intraosseous distractors, which provides a good foundation for domestic production of alveolar distractor and clinical practice of the technique.

采用该骨内型牙槽骨牵张器成功建立稳定的、重复性好的犬牙槽骨牵张成骨动物模型,为牙槽骨牵张器的国产化和临床上应用该项技术打下基础。

Methods The isolated rat primary osteoblast-like cells were exposed to fluid shear stress 2, 4, 8, 12, 14, 17and 19 dyn/cm2 for 60 min respectively in the flow chamber. The ability of proliferation of cells was studied by flow cytometry.

方法对体外培养的大鼠成骨样细胞施加一系列不同大小的流动剪切力,利用流式细胞技术衡量其细胞的分裂增殖能力。

Cumulative data have been documented that mesenchymal stem cells can differentiate into osteoblasts, chondrocytes, adipocytes, tenocytes, myotubes, neural-like cells, hematopoietic-supporting stroma and other non-hematopoietic cells derived from mesoblast or ectoblast.

大量研究表明,体外培养的骨髓间质干细胞在合适的诱导条件下,可分化为成骨细胞、成软骨细胞、脂肪细胞、成肌细胞、成腱细胞和神经元样细胞等多种非造血组织特别是中胚层和神经外胚层来源的组织细胞。

BACKGROUND: The basic fibroblast growth factor has the obvious promotion function of multiplication of the mesoderm and nerve ectoderm cells, and has the indirect ossification to the bone marrow-derived mesenchymal stem cells.

背景:碱性成纤维细胞生长因子对来源于中胚层和神经外胚层的细胞具有明显的促进增殖作用,对骨髓间充质干细胞具有间接的成骨作用。

The modified method, floating explant culture, provides a useful and simple method for the studies of bone biology and bone tissue engineering.

组织块漂浮培养法是一种简便、可行的培养方法,能够为成骨细胞的相关研究提供稳定可靠的种子细胞来源。

We are interested in the relationship between the activity of osteogenesis and hematopoiesis of OGP and Bone Marrow Mesenchymal Stem Cells.

骨髓间充质干细胞是骨髓中一类具有多向分化潜能的干细胞,是骨髓造血微环境的重要组成部分,在适当的条件下可以分化为成骨细胞、软骨细胞、脂肪细胞等。

The biological activity glass has the good bone conduction, the inductivity, can accelerate osteocyte multiplication, causes the osteoblasts to transform into the mature osteocytes.

生物活性玻璃具有良好的骨传导性、诱导性,能加速骨细胞的增殖,使成骨细胞转变为成熟的骨细胞。

Histological analysis From day 14 after the operation, osteoblast-like cells appeared in clusters adjacent to vascular sinuses on either side of the fibrous interzone.

组织学分析术后第十四天,在延长部的纤维组织血管窦附近,可见成簇存在的成骨细胞样细胞,并可观察到类骨组织样基质的出现。

The following data demonstrated that ALP secretion of differentiated hMSC increased significantly compared with undifferentiated cells. And the matrix secreted by differentiated hMSC began to mineralize after 14 days.

结果 经过诱导分化的骨髓基质细胞,碱性磷酸酶分泌明显增加,可以产生大量的矿化结节,具有成熟成骨细胞的表型特征。

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