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Objective: To evaluate the effects of charged surfaces on the activity of bone marrow stromal osteoblasts, and to discover the osteogenic potential of electrets of different surface charges.

目的 研究驻极体产生之静电场对骨髓基质成骨细胞形态及功能的影响,初步探索利用驻极体引导骨定向生长的可能性。

RESULTS AND CONCLUSION: Osteoblast was fusiformed-shaped and had plentiful processes. Nucleus was orbicular-ovate and leaning to lateral side. Soma was large, and plasma was abundant. Alkaline phosphatase staining suggested that a great number of gray-black particles were observed in plasma, and some region was darkly stained.

结果与结论:成骨细胞呈梭形,多突起;细胞核呈卵圆形,偏于一侧;胞体大,胞浆丰富;碱性磷酸酶染色可见胞浆中含有大量的灰黑色颗粒,某些部位染成黑色,定量分析细胞内的碱性磷酸酶、骨钙素水平明显高于成纤维细胞;细胞免疫组织化学染色表明其主要合成Ⅰ型胶原。

MATERIALS AND METHODS: Tooth and bone constructs were prepared from third molar tooth tissue and iliac-crest bone marrow-derived osteoblasts isolated from, and implanted back into, the same pig as an autologous reconstruction. Implants were harvested after 12 and 20 weeks and evaluated by x-ray, ultrahigh-resolution volume computed tomographic, histological, and immunohistochemical analyses.

材料和方法:从第三磨牙和髂骨骨髓获得的成骨细胞的牙齿和骨结构植入自体实验猪体内,12周和20周取材, X 线片,高分辨率 CT ,组织学,免疫组化检测。

There were gene mutations of TGF-1 in SOSP-9607,which might play an important role in pathological mechanism. The gain of muton with strong functional activity is very important for the repair of bone and cartilage injuries.

人成骨肉瘤细胞系SOSP-9607中存在TGF-β1基因突变,其可能在病理机制中起重要作用,具有强功能活性突变子的获得对于骨软骨损伤的修复有重要意义。

RESULTS:① Both induced osteoblasts and MSCs exhibited ALP and calcium node stainings positive.

结果:①诱导成骨分化的骨髓基质干细胞分泌的碱性磷酸酶及钙结节染色均呈阳性。

The compressive pressure, in comparing to the sheas stress, the later, as a kind of physiological like s tress, has less effect on the cellular shape and cytoskeleton of osteoblasts.

静压力作为一种类似于生理状态的外力与剪切力相比,对成骨细胞的形态及细胞骨架影响较轻。

Craniomaxillofacial surgery or accidental injury frequently results in large bony defects requires the bone grafts to enhance the bone healing. Synthetic bone substitutes such as hydroxyapatite, tricalcium phosphate and calcium sulfate have shortcomings in the sense that they have prolonged working and setting time, poor strain, shear strength and not easily shapeable. Therefore, the purpose of this study is to develop a novel, convenient and easily shapeable bone graft that is composed of poly-lactic acid and polycaprolactone as the matrix and calcium sulfate as the filler.

口腔及颅颜外科手术或意外伤害时,倘若造成大范围之骨缺损,常因愈合不易而需要进行骨移植术加速骨的愈合,目前虽有氢氧磷灰石,磷酸钙与硫酸钙合成骨替代物等选择,但这些产品操作及固化时间长、不易塑形、抗张力与抗剪切力较低,而造成手术时间变长,因此本研究目的在研发一种新型且操作便利,可快速塑形,符合组织工程原理,应用於骨缺损的骨?

Craniomaxillofacial surgery or accidental injury frequently results in large bony defects requires the bone grafts to enhance the bone healing. Synthetic bone substitutes such as hydroxyapatite, tricalcium phosphate and calcium sulfate have shortcomings in the sense that they have prolonged working and setting time, poor strain, shear strength and not easily shapeable. Therefore, the purpose of this study is to develop a novel, convenient and easily shapeable bone graft that is composed of poly-lactic acid and polycaprolactone as the matrix and calcium sulfate as the filler.

中文摘要口腔及颅颜外科手术或意外伤害时,倘若造成大范围之骨缺损,常因愈合不易而需要进行骨移植术加速骨的愈合,目前虽有氢氧磷灰石,磷酸钙与硫酸钙合成骨替代物等选择,但这些产品操作及固化时间长、不易塑形、抗张力与抗剪切力较低,而造成手术时间变长,因此本研究目的在研发一种新型且操作便利,可快速塑形,符合组织工程原理,应用於骨缺损的骨?

The induced BMSCs with Dex、β-Gp and AA is a reliable tool for the bone tissue engineer .

骨髓基质细胞作为构建组织工程骨的种子细胞在地塞米松、β-甘油磷酸、L-坏血酸诱导下成骨具有可靠的重复性。

The CBFA1 knockout mice showed no osteogenesis and had maturational disturbance of chondrocytes.

CBFA1基因敲除的小鼠缺乏膜内和软骨内成骨,软骨的成熟及分化也受到了严重干扰。

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