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The purpose of this study was to measure metabolic changes in mesenchymal stem cells placed in osteogenic medium by autofluorescence spectroscopy.

这一研究的目的是通过自身荧光光谱测定在成骨培养基中间充质干细胞的代谢改变。

OBJECTIVE: To observe the effect of long-term culture in vitro on the biological characteristics and osteogenic differentiation potential of adult ADMSCs.

目的:观察体外长期培养对成人脂肪间充质干细胞的基本生物学性状及其成骨分化潜能的影响。

objective: to investigate the effects of lipopolysaccharide on recombinant human osteogenic protein-1 (rhop-1) induced proliferation and differentiation.

目的:研究具核梭杆菌的内毒素对重组人成骨蛋白-1(rhop-1)诱导牙髓细胞增殖、分化的影响。

Objective\ To study the osteogenic property of the induced rabbit bone messenchymal stem cells in nude rats.

目的:研究经矿化诱导的兔骨髓间充质干细胞在裸鼠体内的成骨潜能。

Objective:To explore the optimal system of osteogenic and chondrogenic potential of rabbit bone marrow-derived mesenchymal stem cells.

目的 :探讨兔骨髓间充质干细胞体外分离培养、增殖,以及向成骨细胞和软骨细胞定向诱导分化的条件,为骨、软骨缺损的修复和重建提供种子细胞。

Human amniotic epithelial cells have the property of osteogenous differentiation.

源自足月分娩胎盘的人羊膜上皮细胞具有分化为成骨细胞的特性。

The osteogenous experiment of PCHA: Bone defect models in the distal femurs of rabbits were used in this study.

PCHA在宿主骨内的成骨实验:10只新西兰大白兔,采用国际通用的股骨髁包容性骨缺损模型,填充PCHA骨水泥。

This experiment is sought to study the contribution of different gene in osteogenous differentiation of bone marrow stromal cells and optimize the seed cells of bone tissue engineering.

为了优化骨组织工程种子细胞,探讨不同基因对骨髓基质干细胞成骨活性的影响,我们首先利用RT-PCR方法获得了全长BM P-2,VEGF 165及bFGF基因,克隆入真核表达载体pcDNA 3.0,鉴定正确后,经脂质体介导转入分离培养的大鼠骨髓基质干细胞,G 418筛选出稳定表达株。

MAIN OUTCOME MEASURES: The morphology of BMSCs was observed by the inverted phase contrast microscope. Expression of BMSC surface antigens was detected by flow cytometer. Osteogenous potential was assessed by alkaline phosphatase staining. Adipogenic potential was evaluated by Oil red O staining. Chondrogenic potential was assessed by toluidine blue staining.

主要观察指标:倒置相差显微镜下观察骨髓间充质干细胞的生长状态,流式细胞仪检测细胞表面标志物的表达,采用碱性磷酸酶染色鉴定成骨能力,以油红O染色鉴定成脂能力,以甲苯胺蓝染色鉴定成软骨能力。

ABSTRACT] Objective To establish a stable, useful culture system for human osteoclasts and to investigate the effect of osteoblasts on the differentiation, proliferation and activation of osteoclasts so as to provide a base for the studies on prevention and treatment of osteolysis and osteoporosis.

摘要] 目的建立成人破骨细胞体外培养的方法,观察成骨细胞对破骨细胞分化、增殖和功能的影响,为进一步研究补肾中药防治骨溶解和骨质疏松症提供基础。

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