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The third passage cells had been induced by 0.1 μmol/L dexamethasome, 10 mmol/L β glycerophosphate and 50 μmol/L ascorbic acid 2 phosphate.

取第3代培养细胞用含0.1 μmol/L地塞米松、10 mmol/L β甘油磷酸钠、50 μmol/L维生素C的成骨细胞分化培养液培养。

The passage 3 rat bone marrow mesenchymal stem cells were cultured at the density of 5×107/L in different groups, the induced group was added into conditioned medium containing 1×10-8 mol/L dexamethasone, 10 mmol/L beta-sodium glycerophosphate and 50 mg/L ascorbic acid at the same time of seeding, at 50%-60% confluence, at 85%-90% confluence, respectively.

将第3代大鼠骨髓间充质干细胞以5×107 L-1密度分组培养,诱导组分别于接种当时、细胞达50%~60%融合时、细胞达85%~95%融合时加入含地塞米松、β-甘油磷酸钠、维生素C的成骨诱导培养基诱导。

Results: The iliac bone was in a static state and the osteoblast was scarce.

各年龄组间的成骨细胞计数无统计学差异。

Objective: To examine the number of osteoblast in iliac bone marrow of cleft lip and palate patients of various ages.

目的:初步探讨成骨细胞在不同年龄唇腭裂患者髂骨髓质骨中的存在数量情况。

Transverse innominate osteotomy. D, Cobra plate contoured and attached with two screws for application of compression force. E, Final

截骨术; D 蛇头型钢板弯成骨面的轮廓,用两根螺丝钉固定以利于施加加压力; E 最后固定的钢板; F 钢板和重新固定

Histological examination demonstrated bone formed in accordance with intramembranous ossification in distraction zone and bony connection between the transport disc and mesial mandible on right side; On the left side, there was a big defect at the area of both distraction and defect zone because of dislocated bone transport disc, and no obvious new bone regeneration was found.

组织学检查:见9周后右侧牵张区形成新生骨质,符合膜内成骨,传送盘与近中牵张区形成骨性连接;左侧由于骨传松盘离开缺损区,牵张区与缺损区形成一较大的凹陷,未见明显新骨形成。

Mandibular widening by means of intraoral distraction osteogenesis is an easy, predictable and controlled method that creates new bone.

下颌骨扩大利用内牵引成骨术是一种简单,可预测和控制的方法,创造了新骨。

To establish an experimental dog model of vertical alveolar distraction osteogenesis using intraosseous distractors which were developed by ourselves.

应用自行研制的骨内型牙槽骨牵张器,建立犬垂直向牙槽骨牵张成骨动物模型。

For ADSCs at passage 3, the formation of red l ipid droplets within cells was observed 2 weeks after being induced towards adipocyte, red calcium nodules were evident 3 weeks after being induced towards osteoblast, and highly congregated cell mass positive for alcian blue staining appeared 2 weeks after being induced towards chondrocyte.

第3 代ADSCs 成脂诱导2 周,可见细胞内有红色脂滴形成;成骨诱导3 周,可见红色钙结节样改变;成软骨诱导2 周,可见高度聚集生长的细胞团,阿利辛蓝染色呈蓝色。

Induct cultured cells to differentiate into endothelial cells、lipoblast and osteoblasts in vitro.

对鉴定后的胎盘源间充质干细胞在体外通过特定诱导环境下使之向成脂肪细胞、成骨细胞分化,检测其多向分化潜能。

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呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

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