成骨的

MAIN OUTCOME MEASURES: The proliferation rate and the ALP activity of osteoblasts.

主要观察指标：成骨细胞的增殖率和碱性磷酸酶活性。

Objective: To explore the effects of Gubao-containing serum on ALP activity and OPG mRNA expression in osteoblasts.

目的：观察骨宝含药血清对SD大鼠成骨细胞中护骨素mRNA表达的影响。

The effect of rhBMP-2 on differentiation and proliferation of the stem cells was studied by determination of ALP and MTT activity.

探讨体外条件下rhBMP-2诱导脂肪来源干细胞向成骨细胞分化和增殖的情况，并观察其量效关系。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

In the end, the bioactivity and biocompatibility of samples were studied based on apatite precipitation and osteoblast culture in vitro.

最后，通过体外仿生矿化和成骨细胞培养来研究固定蛋白前后样品的表面生物活性和生物相容性。

Objective To investigate the effect of testosterone on bone cell metabolism.

目的 探讨雄激素对成骨细胞代谢的影响。

Materials and Methods The primary osteoblasts separated from calvarium of 10 fetal rats were cultured and a finite cell line was established in vitro.

材料和方法选用SD胎鼠颅骨作为细胞原代培养的组织来源，建立胎鼠成骨细胞有限细胞系。

METHODS: The calvarium bone of new born rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

METHODS: The calvarium bone of newborn rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added. The proliferation and activity changes of the osteoblasts were identified by counting the cells, determining the ALP activity and observing the changes of cell cycle.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

METHODS: calvarium bone of newborn rats was digested with bacterial collagenase and cultured to obtain osteoblasts and SP with different concentrations was then added. The proliferation and activity changes of the osteoblasts were identified by counting the cells, determining the ALP activity and observing the changes of cell cycle.

采用新生大鼠颅盖骨消化法培养成骨细胞，再加入不同浓度的SP，观察细胞生长数量，测定碱性磷酸酶活性及观察细胞周期变化。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。