成骨的

Alizarin Red-S method was used to study the mineralization of Saos-2 osteoblastic cells.

结果：当应变值为12%时，力学刺激对成骨样细胞Saos-2增殖以及ALP活性的促进作用最强(P.01)。

The presence of calcium deposit activity in cultured cells was demonstrated by Van Kossa staining and alizarin red staining.

在常规培养方法的基础上进行改良，建立组织块漂浮培养法，从新生兔颅骨分离、培养成骨细胞。

MTT method was used to detect the proliferation of the cells. Cell alkaline phosphatase activity and expression of collagen type I were assessed.

另以组织化学和免疫组织化学方法检测传代培养的SD大鼠骨髓基质成骨细胞在PMMA驻极体表面的增殖和分化情况。

In vitro morphological characters,cell proliferation,alkaline phosphatase activity were detected;in vivo the effectiveness of bone formation was evaluated by means of...

体外实验中进行形态学观察、细胞增殖、碱性磷酸酶测定；体内实验通过大体、组织学观察植入物的成骨情况。

In addition, after stably expression of bFGF, the biological characteristics of BMSCs and bone marrow-derived osteoblasts were analyzed in the fields of morphology, proliferation, cell cycle, alkaline phosphatase, typeⅠ collagen and so on.

从形态、增殖特性和细胞周期、碱性磷酸酶、Ⅰ型胶原等方面分析稳定表达碱性成纤维细胞生长因子的骨髓间充质干细胞和骨髓源性成骨细胞生物学特性。

Bone marrow containing hematopoietic stem cells has already been known , theses another group stem cells exists in bone marrow named mesenchymal stem cells. MSCs retain the potentiality of differentiating into osteoblast, chondroblast, adipoblast, tendonblast, myoblast, cardiomyoblast, neuron, etc , which displays a bright prosperity of its use in tissue enigeering, wound healing , cell transplatation and gene therapy. MSCs can be obtained and expanded easily compared to ESC without any problems of morality , law and ethic , immunology.

最早在130年前，德国病理学家Cohnheim在研究创口愈合时就推测在创口愈合过程中出现的部分细胞可能来自骨髓。20世纪70年代，Friendenstein发现一小部分骨髓中贴壁最牢固的细胞经数此传代后形成独特的纺锤形外观，具有形成成骨细胞或软骨细胞的能力。80年代以来，许多研究小组进一步证实了Friendenstein分离到的这些细胞是多能的，在体外特定诱导条件下，可以分化为成骨细胞、软骨细胞、脂肪细胞、成肌细胞、神经元等，被称为骨髓间充质干细胞(Bone MarrowMesenchymal Stem Cells，MSCs)。

This study recruited 8 OI I pedigrees (including a very large OI I kindred comprising 132 members, in which 43 are patients) and 402 nuclear families of health subjects (consisting of 1,263 subjects, and each familycontains at least one female offspring aged 20-45) in Chinese Han population. 1 By performing linkage analysis in the large OII kindred to the COLlAl and COL1A2 genes. Negative LOD score of-8.50 at 9 = 0.001 was observed for the COL1A2 gene, while positive LOD score of 2.31 at 0 = 0.001 was found for the COLlAl gene.

本研究在中国汉族人群中征集了8个Ⅰ型成骨不全家系(其中包括一个拥有132个成员、43个患者的大家系)和402个正常人群的核心家系(共1，263个个体，每一个家系至少含有一个20～45岁的女性子代)。1通过在大的Ⅰ型成骨不全家系中对COL1A1基因和COL1A2基因进行连锁分析，当θ=0.001时，在COL1A1基因处检测到的LOD值为2.31，而在COL1A2基因处检测到的LOD值为-8.50。

But in early resorptive phase in GSB group. The change of BMD, BGP, D-Pyr/Cr and biomechanics index in OVX-PII group were the same as that in OVX-PI group, but the rising range of compressive strength limit in lumbar spine and bend load of femur is bigger than that of JGG, and GSB group. Dynamics osteoblast appeared in large numbers on the surface of os, we can observe the transforming process from osteoblast to osteocyte.

同期预防Ⅱ组的BMD、BGP、尿D-Pyr/Cr及生物力学指标的变化大体维持在预防用药12周时水平，但从数字上看腰椎椎体的压缩强度极限和股骨的弯曲破坏荷载两项的上升幅度均大于健骨颗粒组和骨松宝组，预防II组骨质表面出现成群活跃的成骨细胞，可见成骨细胞往骨细胞转化过程。

To study the function of thyroid hormone receptors in osteoblasts and in bone, the nuclear extracts of TRs from human osteoblast-like cell line MG63 and rat calvaria were achieved, and the two main kinetic parameters of TRs, affinity constant and maximal binding capacity, were determined by radio-ligand binding assay and Scatchard plot.

其中MG63和骨组织中TRs的Ka值与文献报告的人类外周血淋巴细胞以及大鼠脑组织和肝脏中TRs的Ka值相比，差别显著，但与文献中其他成骨细胞系的Ka值相近，提示成骨细胞或骨组织中的TRs与THs的结合能力有可能确实低于淋巴细胞、脑组织或肝脏，从而使得THs在骨组织中的作用机制及效果可能有别于其他组织。

Icariin, the main constituent of Epimedium sagittatum Maxim, and its metabolite icariside Ⅱ showed similar activity in cell proliferation. The total flavonoids from Viscum coloratum Nakai could stimulate cell proliferation and significantly promote cellular ALP activity. Daidzein and its derivatives exhibited no significant activity in cell proliferation.

结果发现：中药淫羊藿中的主要成分淫羊藿苷及其大鼠体内主要代谢物淫羊藿次苷Ⅱ具有类似的作用，可显著促进成骨样细胞的增殖；中药槲寄生总黄酮对成骨样细胞的增殖有一定的促进作用，并可显著提高细胞内ALP活性；大豆苷元及其衍生物对UMR106细胞的增殖作用不明显。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。