成骨的

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。方法：取新西兰白兔骨髓液经离心后得骨髓单个核细胞，以1× 106／ml的细胞浓度进行培养，经传代培养，选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol／l，β-甘油磷酸钠10mmol／l，VitC 50μg／ml)作为骨髓基质细胞增殖和成骨的调节因子，通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase，ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

The immunohistochemistry results indicate that TGF-β1 plays an important role in the distraction osteogenesis.We expect that we can utilize the ectogenesis TGF-β1 to accelerate bone formation in the near future.

有望利用外源性TGF-β1加速牵张成骨中的骨形成，并提高形成骨的质和量，从而为牵张成骨术更好地应用于临床提供理论基础。

Finally, 20 articles concerning treatment progress of distraction osteogenesis and clinical application of implant were included.

从牵张成骨的治疗进展进行总结，对牵张成骨植入物的临床应用进展进行探讨。

Objective:This experiment was designed to establish more feasible and reproducible rat model of mandibular distraction osteogenesis.

目的 ：建立具有良好可行性及可重复性的大鼠下颌牵张成骨模型，为颅面部牵张成骨的深入研究奠定基础。

Effects of Adv-hBMP2 gene modify on the bone formation of tissue-engineered bone substitute. BMSCs of canine transferred by Adv-BMP2 gene expressed ALP in vitro and induced ectopic bone formation in nude mice. BMSCs adhered on the surface of FDB after 4hrs, expanded in 24hrs and proliferated in polylayer in 4d. FDB, combined with BMSCs transferred by Adv-BMP2 gene, induced bone formation in the subcutis of nude mice in 6 weeks.

结果：1.BMP-2基因修饰对组织工程化骨成骨的影响 BMSCs经Adv-BMP2基因转染后ALP染色强阳性、并在裸鼠肌肉形成异位骨组织；转基因的BMSCs与冻干骨复合后24小时即完全贴附生长，4天后复层生长并分泌大量胶原，植入裸鼠背部皮下6周后可见大量成骨，很少吸收，而单纯冻干骨或冻干骨复合BMSCs基本不成骨。2。

Abstract］objective: effects of a single period of mechanical strain on gene expression patterns of two osteogenic transcription factors, ets1 and cbfa1, in rat calvarial sutural osteoblast-like cells were studied in this study to elucidate the molecular mechanism of suture distraction.

摘要］ 目的：探讨大鼠颅骨缝成骨细胞在张应力作用下ets1和cbfa1基因表达的变化，以揭示骨缝牵张成骨的分子机制。

Results:(1) CBC possesses a natural trabecular structure. Its components is similar to the mineral of bone.(2) CBC has great cellur biocompatibility. It can promote cell adhesion, proliferation and secretory while needn t modification.(3) In nude mice, the ectopic osteogenesis of MCCAB was obvious which was better than that of CBC alone and the new bone formation increased in pace with time. In the MCCAB, both intramembranous and cartilaginous osteogeneses were seen but the former dominated.(4) When MCCAB was autotransplanted intramuscularly, the osteogenesis was much better than that of negative control groups.(5) The MCCAB can repair autogenous calvaria defects effectively and the effect was much better than that of CBM alone and blank group. Conclusion:(1) CBC possesses good biocompatibility, osteoconduction and porous structure, can be used as scaffold material for constructing of tissue engineering bone.

结果：(1)CBC具有天然的多孔网状结构，最大程度地保持了骨矿的微结构特征，可以作为骨组织工程的支架材料；(2)CBC细胞亲和性好，不需作修饰即能促进成骨细胞的粘附、增殖和分泌活动；(3)MSO/CBC复合人工骨在裸鼠皮下异位成骨作用明显，兼有膜内成骨和软骨内成骨的特点，成骨效果明显优于单纯CBC植入组；(4)MSO/CBC复合人工骨兔自体肌袋异位成骨作用明显，血管化充分，成骨效果明显优于单纯CBC植入组；(5)MSO/CBC复合人工骨修复兔颅骨临界性缺损效果好，明显优于单纯植入CBC组和空白对第四旱区大学硕士学位论文一照组，可以作为骨移植替代材料用于骨缺损畸形修复。

This study investigated the biologic properties of artificial synthetic HAP, the osteogenous capacity of red bone marrow after being transplanted to a heterotopic site, and the worth of composite HAP-BM implanted to osteogenesis.

本文研究包括：人工合成的HAP的生物反应，自体BM异位移植的成骨和诱导成骨作用和HAP配合BM移植对成骨的价值。

ABSTRACT A Clinical and Experimental Study on Trifocal Distraction Osteogenesis for Reconstruction of Segmental Mandibular Defects

探讨三焦点牵引成骨技术在下颌骨骨段缺损功能性重建中的应用，研究三焦点牵引成骨的成骨规律、特点和重建骨种植义齿修复的可行性。

After four weeks most gelatin sponge were resorbed and grafts had been replaced by vascularised fibreus tissue and had not formed any new bone.

究其原因是HAP提供成骨的支架，而BM提供成骨细胞，和诱导成骨物质，两者需同时使用，缺一不可。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。