成骨

RESULTS:① Both induced osteoblasts and MSCs exhibited ALP and calcium node stainings positive.

结果：①诱导成骨分化的骨髓基质干细胞分泌的碱性磷酸酶及钙结节染色均呈阳性。

The compressive pressure, in comparing to the sheas stress, the later, as a kind of physiological like s tress, has less effect on the cellular shape and cytoskeleton of osteoblasts.

静压力作为一种类似于生理状态的外力与剪切力相比，对成骨细胞的形态及细胞骨架影响较轻。

Craniomaxillofacial surgery or accidental injury frequently results in large bony defects requires the bone grafts to enhance the bone healing. Synthetic bone substitutes such as hydroxyapatite, tricalcium phosphate and calcium sulfate have shortcomings in the sense that they have prolonged working and setting time, poor strain, shear strength and not easily shapeable. Therefore, the purpose of this study is to develop a novel, convenient and easily shapeable bone graft that is composed of poly-lactic acid and polycaprolactone as the matrix and calcium sulfate as the filler.

口腔及颅颜外科手术或意外伤害时，倘若造成大范围之骨缺损，常因愈合不易而需要进行骨移植术加速骨的愈合，目前虽有氢氧磷灰石，磷酸钙与硫酸钙合成骨替代物等选择，但这些产品操作及固化时间长、不易塑形、抗张力与抗剪切力较低，而造成手术时间变长，因此本研究目的在研发一种新型且操作便利，可快速塑形，符合组织工程原理，应用於骨缺损的骨？

Craniomaxillofacial surgery or accidental injury frequently results in large bony defects requires the bone grafts to enhance the bone healing. Synthetic bone substitutes such as hydroxyapatite, tricalcium phosphate and calcium sulfate have shortcomings in the sense that they have prolonged working and setting time, poor strain, shear strength and not easily shapeable. Therefore, the purpose of this study is to develop a novel, convenient and easily shapeable bone graft that is composed of poly-lactic acid and polycaprolactone as the matrix and calcium sulfate as the filler.

中文摘要口腔及颅颜外科手术或意外伤害时，倘若造成大范围之骨缺损，常因愈合不易而需要进行骨移植术加速骨的愈合，目前虽有氢氧磷灰石，磷酸钙与硫酸钙合成骨替代物等选择，但这些产品操作及固化时间长、不易塑形、抗张力与抗剪切力较低，而造成手术时间变长，因此本研究目的在研发一种新型且操作便利，可快速塑形，符合组织工程原理，应用於骨缺损的骨？

In the whole life of human being, bone is always in the process of new-old alteration, which means osteoclast absorbing old sclerotin and osteoblast forming new sclerotin.

人的一生中骨骼一直处于新旧更替的过程中，即由破骨细胞吸收旧的骨质，成骨细胞形成新的骨质。

The cell morphology was observed by phase contrast microscope and the cell proliferation was determined by MTT assay.

在PLA、EMPLA及玻璃上培养成骨细胞，采用细胞形态学观察法和细胞增殖法，在相差显微镜下观察细胞在上生长情况；分别于1、2、4、6d用MTT法记数，绘制生长曲线图。

Osteoblasts were collected from the rabbit fetus skull by collagenase digestion associated with tissue nubbles cultivation and cultured in vitro.

酶消化-组织块联合培养法体外培养胎兔颅骨成骨细胞：取新西兰大白兔胎兔颅骨骨块，制成2mm 2mm骨块，Ⅰ型胶原酶室温下消化。

In this study, weight-bearing bone was engineered using osteogenically induced BMSCs.

本实验采用成骨诱导的骨髓基质细胞修复承重骨。

The induced BMSCs with Dex、β-Gp and AA is a reliable tool for the bone tissue engineer .

骨髓基质细胞作为构建组织工程骨的种子细胞在地塞米松、β-甘油磷酸、L-坏血酸诱导下成骨具有可靠的重复性。

Methods: Osteoblasts were isolated from parietal bone of the newborn SD rats (less than 24 hours) and cultured in minimum essential medium.

成骨细胞由新生24h SD大鼠头盖骨分离得到。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐