成骨

Methods Porous heterogenous deproteinication boneof pig obtained by physiochemical process and osteoblast acquired from the cranical bone of fetal rabbits were cultured 10 days in vitro and explanted into the left side of dorsa muscle of 30 adult rabbits.

将经过物理化学方法处理制得的冻干猪脱蛋白型松质骨与胎兔颅骨来源的成骨细胞体外培养 10d后，在无菌条件下植入 3 0只成年新西兰兔背部肌肉内，对照组为同体单纯植入猪脱蛋白型松质骨。

The inhabiting effects of steteching at 1500μεwere more significant than that of 1000με.

拉伸对成骨细胞生理活性的影响随加载时间的增加而增加。

Rabbits (48 laterals) were prepared as model of bone defect, divided into 3 groups, named complex group (24 laterals), in which, unilateral bone defect of 24 rabbits were implanted with cell + carrier complex; The rtificial bone group (12 laterals), in which, CPAB was implanted contralaterally;and blank control (12 laterals), in which, no any management was done contralaterally in bone defect of 12 rabbits.

24只兔(48侧)制作成骨缺损模型，分为3组，复合物组(24侧)，24只兔一侧骨缺损植入细胞+载体复合物；人工骨组(12侧)，12只兔对侧植入磷酸钙人工骨；空白对照组(12侧)，12只兔对侧骨缺损不作任何处理。

Lysed cells were cultured for bacterium colony forming unit,unlysed cells were sent for EM examination.

部分被感染的成骨细胞溶解后用于细菌培养，计算菌落数，部分送电镜观察。

Breast cancer commonly metastasizes to bones, producing both osteolytic and osteoblastic deposits. Different markers for quantitative determination of bone turnover have been developed to evaluate bone metastases of breast cancer. The urinary deoxypyridionline, a crosslink product of collagen molecules found in bone and excreted in urine during bone degradation, and bone specific alkaline phosphatase, an isoenzyme localized in the membrane of osteoblasts and released in circulation during bone formation, were recently described as a group of markers of bone turnover in metastatic cancer.

乳癌患者常见骨髓转移造成溶骨性及成骨性沈积物，而利用不同标记来定量骨质转换形态，可以评估乳癌骨骼转移的变化，而尿液中Deoxypyridinoline是骨质内胶原分子在骨骼破坏分解时至尿中的产物，骨特殊碱性磷酸酵素是一种同形酵素，位於成骨细胞的外膜且在骨骼形成时释放至血液中，利用此两种生化标记，可以来预测乳癌转移至骨骼的转换情形。

For this purpose, it is necessary to create scaffolds that can be degraded by osteoclasts and enable osteoblasts to build new mineralized bone matrix.

为了这一点，人们有必要创造一种支架，它不仅可以被破骨细胞降解也能让成骨细胞在其中生成新的矿化骨基质。

This tissue constitute an ideal source of osteoblasts and mineralized tissue for bone regeneration.

该组织为骨再生过程中成骨细胞及矿化组织提供了理想来源。

METHODS: The osteoblasts from cranial bones of 5-day-old Wistar mice were seeded and passaged.

用出生5d的Wistar品系小鼠的颅骨接种成骨细胞并传代。

BMSCs cultured in vitro contain osteogenic precursor cells, and the passaged cells possess osteogenic potential.

体外分离培养的BMSCs中含有骨源性前体细胞，传代细胞具有较强的成骨潜能。

The expression of ZUB1 antigen had no difference after passaging,but it was significant decreased after differentiated into adipocytes and osteocytes,which indicated that the ZUB1 antigen may be an important marker of "stem" and was associated with the differentiation of MSCs.

当诱导骨髓MSCs向成骨细胞和脂肪细胞定向分化时，流式细胞检测显示ZUB1抗原表达相应减弱，提示ZUB1抗原可作为MSCs干细胞特征的标志分子，且可能参与了MSCs分化的细胞活动。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。