成骨

Qianggubao Formula; osteoplast; multiplication; serum of diabetes rat model; time-phase and quantity-phase; culture in vitro

目的：探讨强骨宝方糖尿病模型鼠含药血清促进体外培养成骨细胞增殖的最佳时相与量效。

OBJECTIVE: To explore the feasibility of adipose-derived stromal cells in repairing loaded bone defects through observing the repair of rabbit tibial bone defects with adipose-derived stromal cells that was cultured to osteoplast and bovine decalcified bone composite.

目的：通过兔脂肪基质细胞诱导培养为成骨细胞，并与牛脱钙骨复合培养，对兔胫骨骨缺损修复情况的观察，探讨脂肪基质细胞修复骨缺损的可行性。

The iliac bone defect moulds were prepared and the compound scaffolds were implanted. The compound scaffolds were taken out after four and eight weeks separately, bone healing status and appearance of scaffolds were observed by eyes, the osteoplast growth status in compound scaffolds were viewed by HE and chinalizarin dyeing separately, the degradation of the compound scaffolds in vivo were observed by SEM.

制作髂骨骨缺损模型，在骨缺损处植入骨支架复合体，植入后4周、8周取材大体观察材料与周围骨愈合情况，HE染色及茜素红染色观察支架复合体内成骨细胞生长及扫描电镜观察支架体内降解情况。

Spindle shape of cells gradually disappeared, with increased cell body. Some cells grew in cluster. MSCs changed figures to multilayer knots 10 days after inducing osteoplastic differentiation.

骨髓间充质干细胞向成骨分化过程中，纺锤形的突起逐渐消失，胞体增大，培养10 d后，部分细胞呈聚集生长，随细胞生长密度的增长形成多层的结节结构。

MAIN OUTCOME MEASURES: Morphological changes in primary cultured and subcultured mouse BMSCs were measured. BMSC surface antigen of the second passage of mice (CD105, CD44, CD25, CD34) were determined using flow cytometry. The modified method was assessed to harvest the purity of stem cells. Activity of mouse BMSCs was identified using adipogenic and osteoplastic differentiation. The strength of fluorescent cells following multiple passage was observed.

主要观察指标：原代及传代小鼠骨髓间充质干细胞形态变化；对第2代小鼠骨髓间充质干细胞表面抗原，CD105，CD44，CD25，CD34进行流式细胞仪检测，评价改良方法获取干细胞纯度；通过成脂成骨分化，鉴定小鼠骨髓间充质干细胞的活性；观察多次传代后荧光细胞强度。

After osteoplastic induction, peripheral blood MSCs had strongly positive reactions for alkaline phosphatase staining, total collagen staining, alizarin red staining.

外周血间充质干细胞成骨诱导后，碱性磷酸酶染色、总胶原染色及茜素红染色均呈强阳性；成脂诱导后油红染色呈阳性，有一定数量的脂滴形成。

The decreased expression of TGF-β1 may be a reason of decreased quality of osteoporotic fracture healing.

3TGF-β1在成骨细胞中的表达减少是骨质疏松和骨折愈合质量下降的可能因素之一。

PURPOSE: This study is aimed to evaluate the application of three-dimensional simulation and virtual osteotomy guide in distraction osteogenesis.

目的：探讨维软硬组织模拟以及应用虚拟截骨导板将手术设计转移至牵张成骨术的效果。

Objective To provide experiment evidence for clinical practice and for further exploration on mechanism and medicinal control of periapical diseases.

目的 观察氢氧化钙对骨组织成骨活性的影响，为根尖周病药物治疗学提供实验依据。

It might be related to the content of red bone marrow and the capability of periosteal bone formation.

可能与红骨髓的含量和骨膜的成骨能力有关。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。