成骨

Although numberous experiments and clinical studies on DO appeared,thereare still some questions unclear,including long duration of treatment and incidence of complications.

尽管牵张成骨技术用于矫治颅颌面先天发育畸形和获得性骨节段缺损已获得成功，但是它需要较长的总体治疗时间，给患者带来不便，而且有感染、骨愈合不良甚至骨折的危险。

Methods BMSCs isolated from rabbit iliac bone marrow were culture-expanded in vitro. The injectable tissue-engineered bone constructed from autologous PRP, FG, and BMSCs was cultured in vitro, and its biological characteristics were observed including the time of gel formation, histological features, seed cell survival and microscopic structures. Results The constructed injectable tissue-engineered bone began gel formation within 20 to 30 s, and after a week-long culture, the gelatine began to degrade, and numerous well viable fusiform cells could be seen to adhere to the bottom of the Petil dish, Scanning electron microscopy identified globular and olivary cells embedded in the fibrin glue, and numerous small particles could be seen around of the cells.

从兔髂骨处抽取骨髓体外培养BMSCs并诱导向成骨细胞分化，抽取兔自体动脉血提取PRP，以FG、BMSCs、PRP共同构建可注射型组织下程骨并体外培养观察其生物学特性如凝胶形成时间，组织学特点、细胞存活情况及其超微结构特征等结果构建的可注射型组织工程骨可在短时间内形成凝胶，体外培养1周时其中细胞生长良好，电镜下见纤维蛋白网格结构致密，种子细胞及血小板颗粒分布广泛。

METHODS:① MSCs were isolated from dog bone marrow by density gradient centrifugation, followed by in vitro adherent culture and ontogenesis culture.

①采用密度梯度离心法从犬骨髓中分离骨髓基质干细胞，体外贴壁培养扩增、成骨诱导培养。

Results:The cells were growing、proliferating、ossific differentiating well on the scaffold.

结果：细胞在支架上粘附、增殖、成骨分化。

MAIN OUTCOME MEASURES: In vivo degradation performance and ossific capability of PHBV copolymer as well as energy-dispersive X-ray analysis and elemental quantitative assay in bone defect regions.

主要观察指标：①聚羟基丁酸/羟基戊酸共聚酯的体内降解性能和成骨能力。②骨缺损区X射线能谱分析及元素定量测定。

These results showed that there were significantly different effects of AGE-BSA on cell proliferation between human and rats' osteoblastic cell.

以上结果表明：AGE对人和大鼠的成骨细胞增殖的诱导作用相差很大。

OBJECTIVE: To investigate the proliferation and osteoblastic differentiation of adipose-derived stem cells induced by calcitonin gene-related peptide in vitro.

目的：探讨降钙素基因相关肽在体外定向诱导脂肪干细胞向成骨细胞增殖分化的可行性。

OBJECTIVE: To study the interaction between tumor necrosis factor-α and osteoprotegerin on the apoptosis of osteoblastic cells.

目的：探讨肿瘤坏死因子α能否诱导成骨类细胞凋亡及骨保护素能否抑制其凋亡。

Genistein can stimulate human osteoblastic cell proliferation and differentiation in a dose and timedependent manner.

染料木黄酮能够促进人成骨细胞的增殖和分化，其促进作用存在时效和量效关系。

Recent progress has been made in the identification of the osteoblastic cellular niche for hematopoietic stem cells within the bone marrow.

最近在识别骨髓中造血干细胞的成骨细胞龛方面方面取得了新的进展。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。