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Methods: The multinucleated osteoclasts were derived by 1,25-dihydroxyvitamin D3 and dexamethasone from bone marrow cells in the coculture system with primary osteoblastic cells.

采用原代培养的成骨细胞和骨髓单核细胞联合培养的方法,在1.25-2维生素D3和地塞米松作用下,使骨髓单核细胞分化形成破骨细胞。

This review focused on the biological significance of matrix metalloproteinases in its regulation when mesenchymal stem cells differentiate into myoblastic, osteoblastic, chondroblastic, neural, and apidoblastic lineages.

文章详细阐述了基质金属蛋白酶在间充质干细胞分化为成肌细胞、成骨细胞、破骨细胞、神经细胞、脂肪细胞过程中所起的重要生物学作用,并对基质金属蛋白酶在间充质干细胞分化中的调控作用进行分析。

To observe the effect of naringin solution on osteoblast line MC3T3E1 proliferation.

检测柚皮苷溶液对成骨细胞株MC3T3E1细胞增殖功能的影响。

Control group: inflammatory cell infiltration (1W); inflammatory cell disappearance, fibroblast and capillary tachyauxesis (2W); osteoblast appearance around bone defect (1M); small amount neoformative bone tissue around bone defect.

对照组:术后1周,骨缺损区周围有大量炎性细胞浸润;术后2周,炎性渗出吸收,成纤维细胞、毛细血管生长活跃;术后1个月,骨缺损区周围有成骨细胞;术后3个月,骨缺损边缘有少量新骨沉积。

Control group: inflammatory cell infiltration (1W); inflammatory cell disappearance, fibroblast and capillary tachyauxesis (2W); osteoblast appearance around bone defect (1M); small amount neoformative bone tissue around bone defect. Conclusion TGF-β1 can induce alveolar bone regeneration.

对照组:术后1周,骨缺损区周围有大量炎性细胞浸润;术后2周,炎性渗出吸收,成纤维细胞、毛细血管生长活跃;术后1个月,骨缺损区周围有成骨细胞;术后3个月,骨缺损边缘有少量新骨沉积。

ConclusionSensory nerval bundles and vascular bundles can effectively accelerate the ossification of tissue engineered bones.They can be used as a construction method for nervalization after tissue engineered bone.

结论感觉神经束和血管束具有明显的促组织工程骨成骨作用,可以作为组织工程骨神经化构建的一种方法。

BACKGROUND: Bone marrow mesenchymal stem cells can differentiate into osteoblasts, chondrocyte, sarcoblasts, adipocyte, neuroepithelial cells, as well as vascular endothelial cells under different conditions.

背景:骨髓间充质干细胞在不同条件下可以分别分化为成骨细胞、软骨细胞、成肌细胞、脂肪细胞、神经胶质细胞、血管内皮细胞等。

Specimens of tibial plateau were retrieved 3 months and 6 months after operation. X-ray and tibia plateau compression rigidity were tested. Degradation of ICHA cement and new bone formation were evaluated in nondecalcified section.

术后3个月和6个月时取材,拍X光片,检测胫骨平台骨折块抗压刚度,制备不脱钙切片,观察ICHA水泥和自体骨的吸收与成骨情况,进行骨计量学静态参数与动态参数的检测。

AIM: To observe the regulative effects of different concentrations of glucose on the expressions of tumor necrosis factorrelated apoptosisinducing ligand,osteoprotegerin,and the ligand of osteoprotegerin in osteosarcoma MG63 cells,and to study the role of nonphysiological concentration of glucose in pathogenesis of diabetic osteoporosis.

目的:观察不同浓度葡萄糖环境下人成骨肉瘤MG63细胞株肿瘤坏死因子相关凋亡诱导配体及其护骨素、护骨素配体的表达,探讨糖尿病骨质疏松症的发病机制。

N Dq'^8z?1I3q AIM: To observe the regulative effects of different concentrations of glucose on the expressions of tumor necrosis factorrelated apoptosisinducing ligand,osteoprotegerin,and the ligand of osteoprotegerin in osteosarcoma MG63 cells,and to study the role of nonphysiological concentration of glucose in pathogenesis of diabetic osteoporosis.

目的:观察不同浓度葡萄糖环境下人成骨肉瘤MG63细胞株肿瘤坏死因子相关凋亡诱导配体及其护骨素、护骨素配体的表达,探讨糖尿病骨质疏松症的发病机制。

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