成骨

OBJECTIVE: To label rabbit BMSCs by green fluorescent protein, and then to observe the potency of multi-directional differentiation by osteoblast and lipoblast formation induction.

目的：采用绿色荧光蛋白标记兔骨髓间充质干细胞，观察标记后其诱导成骨及成脂能力。

Results In define induction envioroment, HPMSCS can diferentiate into lipoblast、after 2 weeks, the induced cells are full of lipid droplet;HPMSCS can differentiate into osteoblasts, after 4 weeks, we can see calcified tubercle in the cells, calcium in the calcified tubercle can be dyed black by Von Kossa and ALP dying is positive reaction above 95%.

结果 HPMSCS在培养基中持续培养后，在特定诱导环境下，成功使其向成脂肪细胞转化，培养2周时，可见融合成团的脂滴充满整个细胞；成功使其向成骨细胞转化，培养4周以上可见明显钙化结节，von Kossa染色可将骨结节中沉积的钙染成黑色，碱性磷酸酶染色呈强阳性反应达到95%以上。

Result: The Mixed group including morroniside,sweroside and loganin with different dose all significantly promoted pro.

结果：六味地黄丸主要血中移行成分莫诺苷、獐牙菜苷、马钱子苷的混合物各剂量组均明显表现出对大鼠成骨细胞的促增殖作用。

Result: The Mixed group including morroniside, sweroside and loganin with different dose all significantly promoted proliferation of rat osteoblast.

结果：六味地黄丸主要血中移行成分莫诺苷、獐牙菜苷、马钱子苷的混合物各剂量组均明显表现出对大鼠成骨细胞的促增殖作用。

Methods The CXB combining BM was implanted into the sacrospinalis muscle of rabbits, and CXB implanted alone was used as control. Eighteen Japanese rablits with longear were used. The size of CXB was 5 mm×5 mm×5 mm, and the implanted materials were taken out at 2、 4、 8、 12、 16 and 24 weeks after implantation.

将CXB与BM复合及单纯CXB植入兔的骶棘肌内，术后2、4、8、12、16及24周取出植入材料作组织学及组织化学检查，观察植入材料的成骨作用。

At 54 weeks, plentiful growing bone and bone marrowlike tissue fully filled in pores created by biodegradation of CPC/CS.

方法试验分CPC／CS及CPC两组，三点弯曲试验测试材料的强度和弹性模量；组织学观察材料植入兔股骨22及54周的成骨状况。

But in maxillofacial bones, it only has about 20 years clinical and experimental study.

牵张成骨技术在四肢骨中的应用由来已久，但对于颌骨DO的实验及临床研究只有二十多年的历史。

Mesenchymal stem cells are multipotent cells derived from many adult tissues, which can differentiate into cells of the mesodermal lineage, such as adipocyte, osteocyte and chondrocyte, as well as cells of other embryonic lineages. They are a promising tool for tissue engineering.

间充质干细胞是一类可从多类成体组织中分离的能向脂肪细胞、成骨细胞和软骨细胞等分化的成体干细胞，是组织工程细胞的一个理想来源。

BACKGROUND: Under different induction conditions, bone marrow mesenchymal stem cells can differentiate into the mesodermal tissues such as osteoblasts, chondroblasts, muscle cells, adipocytes and so on.

背景：骨髓间充质干细胞在不同诱导条件下具有向中胚层组织细胞如成骨细胞、成软骨细胞、肌细胞、脂肪细胞等分化的能力。

OBJECTIVE: To assess the value of mandibular distraction osteogenesis in treatment of OSAS in patients with congenital micrognathia and the effect of the surgery on the patients' respiratory functions.

目的：探讨和评价下颌骨牵引成骨技术在治疗小下颌畸形伴OSAS中的应用价值及对呼吸功能的改善作用。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。