成骨

The third passage cells had been induced by 0.1 μmol/L dexamethasome, 10 mmol/L β glycerophosphate and 50 μmol/L ascorbic acid 2 phosphate.

取第3代培养细胞用含0.1 μmol/L地塞米松、10 mmol/L β甘油磷酸钠、50 μmol/L维生素C的成骨细胞分化培养液培养。

Objective To investigate whether osteoblasts cultured in vitro can form mineralization nodes. Method The osteoblasts at 1×105/ml,2×105/ml and 5×105 /ml cell density were cultured in the DMEM medium and in the DMEM medium supplemented with β-glycerophosphate sodium and ascorbic acid.

目的 了解体外培养成骨细胞的接量对钙化结节形成的影响方法实验选用了1×105 /ml、2×105个/ml、5×105个/ml三个不同的细胞接种浓度，并且每个浓度组分别给予含β-甘油磷酸钠和维生素C的条件培养液以及常规培养液进行细胞培养。

Cultured osteoblasts at suitable cell density can easily form mineralization nodes in vitro, and β-glycerophosphate sodium and ascorbic acid just promote formation of mineralization nodes.

体外培养成骨细胞钙化结节的形成须接种适宜的细胞数量，用条件培养液培养细胞能促进钙化结节形成。

Methods: Osteogenic medium (expansion medium with 10 mmol·L -1 dexamethasone,50 μg·L -1 ascorbic acid and 10 mmol·L -1 β glycerophosphate ) was used to stimulate MSCs into osteoblast like cells.

选用生长状态良好的兔骨髓间质干细胞，在体外进行成骨活性诱导，并进行形态学观察和碱性磷酸酶、骨钙素的测定。

Results The results indicated that the cultured human alveolar bone origin cells from 3 patients could successfully express the osteoblasts phenotype in single layered culturing after stimulated by β-glycerophosphate and Dexamethasone.

结果自3人下颌齿槽骨分离培养的齿槽骨细胞在加入β-磷酸甘油和地塞米松刺激的体外培养条件下，均可成功表达成骨细胞的表现型，与同源成纤维细胞相比，差异有显著性(P.05)。

The passage 3 rat bone marrow mesenchymal stem cells were cultured at the density of 5×107/L in different groups, the induced group was added into conditioned medium containing 1×10-8 mol/L dexamethasone, 10 mmol/L beta-sodium glycerophosphate and 50 mg/L ascorbic acid at the same time of seeding, at 50%-60% confluence, at 85%-90% confluence, respectively.

将第3代大鼠骨髓间充质干细胞以5×107 L-1密度分组培养，诱导组分别于接种当时、细胞达50%~60%融合时、细胞达85%~95%融合时加入含地塞米松、β-甘油磷酸钠、维生素C的成骨诱导培养基诱导。

OBJECTIVE: To investigate the effect of Jiangu granula on the apoptosis of rat osteoblast induced by TNF-ɑ.

目的：验证健骨颗粒含药血清对肿瘤坏死因子α诱导的鼠成骨细胞凋亡的影响。

The present invention can make osteoplast and hemopoietic stem cell well grow to result in cell proliferation of 23 times and 49 times separately in 10 days, and is suitable for large scale culture of cells.

本发明的效果和益处是可使成骨细胞及造血干细胞在此系统中良好生长，十天后细胞的扩增倍数分别可达到二十三倍和四十九倍，适合细胞的大规模培养。

RESULTS: At 16 weeks after operation, the defects were perfectly healed in the composite group, which was better than heterogenic deproteinized cancellous bone and control group with regard to the levels of bone formation, quantity of bone regeneration and reconstruction of marrow cavity.

结果：植入后16周，复合材料组骨缺损完全修复，成骨活跃程度、骨再生量和重建髓腔结构等方面均显著优于异种脱蛋白松质骨组和空白组；异种脱蛋白松质骨组，形成骨性愈合，但无髓腔再通；空白组未愈合。

Methods The specimens which were identified as osteosarcoma were cultured primarily in vitro. The surviving cells underwent morphological observation, histochemical staining, isoenzyme pedigree determination, cell cycling analysis, karyotype analysis and heterotransplantation.

采用原代组织块培养法对成骨肉瘤手术标本进行培养，对存活细胞进行形态学观察、组织化学染色、乳酸脱氢酶同工酶谱、细胞周期检查、核型分析、异种移植。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。