成骨

Study on culture of osteoblasts from neonatal rat's calvaria in simulated weightlessness.

利用回转器建立模拟失重环境下大鼠颅骨成骨细胞体外培养模型。

Objective: To investigate the effects of recombinant human insulin-like growth factor on the proliferation , apoptosis and anabolic function of cultured rat osteoblasts in vitro.Method: Rat calvaria cells were cultured in medium with or without rhIGF-I.

目的 通过观察重组人胰岛素样生长因子I对体外培养的大鼠成骨细胞增殖、凋亡和功能的影响，探讨rhIGF-I对骨代谢影响的可能机制。

Growth characteristcis of primary and passage cells obtained from human fetal calvaria were observed with an inverted microscope.

用碱性磷酸酶染色、钙结节茜素红染色等方法对所获得的成骨细胞进行鉴定。

METHODS: Using the method of enzyme digested, Osteoblasts obtained from newborn rat calvaria were cultured for serial passage.

采用酶消化法从新生SD大鼠头盖骨分离成骨细胞，传代培养。

The results showed that:a Cyclic strtching at 500με,1000μεand 1500μεwith the frequency of 0.4Hz affected the physiological activity of osteoblasts delivered from rat calvaria.

应变分别为500με、1000με和1500με，频率为0.4Hz的周期性拉伸刺激影响大鼠颅盖骨中分离出的成骨细胞的生理活性。

In order to get a more purification of osteoblast, Osteoblastic cells are isolated by sequential enzyme digestion from newborn SD rat calvaria.

为获得比较纯化的成骨细胞，本实验用新生1～2天的SD大鼠颅盖骨，采用二次胶原酶消法化进行细胞体外培养。

Methods Rat calvaria osteoblasts were cultured in the presence of 10 μg/mL-500 μg/mL nHA or nAg-HA/TiO2 for 2 h, 6 h, 8 h, 24 h, 72 h and 120 h. Cell proliferation, vitality and ultrastructure were tested using cell counting chamber, MTT assay, and transmission electron microscope.

将成骨细胞分别在添加了不同浓度(10μg/mL~500μg/mL)的nHA或nAg-HA/TiO2的培养基中培养2、6、8、24、72及120h，通过透射电镜观察、细胞计数及MTT法检测细胞超微结构、细胞增殖及线粒体活性，并计算单位细胞呼吸率。

The high-affinity binding of ~125I-triiodothyronirie to the nuclear extracts was demonstrated, and Ka of TRs in MG63 cell line and in rat calvaria we...

另外MG63和骨组织中TRs的MBC也存在一定差别，提示体外培养的人类成骨样细胞系中TRs数量可能减少。

Newborn rat calvaria osteoblastic cells were incubated with TNF-α(20、30、40ng/ml) for 24 and 48h.

用TNF-α 20、30、40ng／ml的量刺激成骨细胞，观察24、48小时，测定细胞培养液中亚硝酸根含量。

Methods:The cattle cancellus bone were degreased,deproteined and calcined to form TBC vector.After the surface was covered with collagen.the cultured rabbit osteoblasts were compounded to TBC in vitro.5,10 and 20 day later,the compound materials were observed under scanning electron microscorpe.

牛松质骨经脱脂、脱蛋白、煅烧等工艺制成煅烧骨作为载体，载体表面经胶原处理后将体外培养的兔骨膜成骨细胞复合到TBC，分别于5日，10日和20日取材，行扫描电镜观察，设对照组。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。