成骨

TRIG and CHOL began increasing after one week of injection, which was obvious with prolongation of injection.

VEGF在成骨细胞、软骨细胞中的阳性表达率与血液流变学和MSCT灌注成像指标均不相关。

Methods New Zealand rabbits 10 weeks of age were selected to extract bone marrow solution at greater trochanter of femur.

目的 探讨兔骨髓基质细胞的体外分离培养及定向诱导成骨的方法。

The defect was repaired with the osteochondral plug with the same diameter and depth taken from the proximal femoral trochlea using the OATS. The contralateral femoral trochlea was treated as normal control.

18 只新西兰兔右膝股骨滑车造成骨软骨缺损，取滑车临近部位相同直径和厚度的骨软骨柱修复缺损，左膝作为正常对照。

Then the cultivated chondrocytes were embedded in fibrin glue fused on spongy bone, covered with priosteal flap; the complex was used to repair the femoral trochlea osteochondral defect which size is 3mm × 4mm × 4mm made in rabbit knee joint.

在A组的每只兔子的一侧膝关节股骨滑车部人为造成3mm×4mm的骨软骨缺损，骨刀切除软骨下骨到髓腔渗血为止(厚约4mm)，压迫后FG止血；取髂骨骨块，并尽可能保留松质骨，取下的骨块用PBS反复清洗，以除去血细胞，将松质骨填充在骨缺损处，松质骨面朝向关节腔，高度与周边软骨下骨齐平，把骨膜片生发层朝向关节腔，用无创伤缝合线缝合在周边的软骨或滑膜组织上；向EP管中加入1/2悬液体积的FG主体胶溶液并混匀，再与主体胶等体积的催化剂溶液一同注射入骨膜与骨块密闭的腔隙中；同理处理另一侧膝关节。B组处理与A组相比只是不加入软骨细胞；C组造成骨软骨缺损，FG覆盖创面后单纯用骨膜修复缺损。

In order to avoid prolin residue inhibiting enterokinase cleavage, 9bp of MGF cDNA 5′ end sequence was truncated by primer, then the obtained truncated MGF des(1-3MGF cDNA (321 bp) was subcloned in pET32a vector to construct a prokaryotic recombination expression plasmid.

通过RT-PCR从拉伸刺激的人成骨细胞中克隆MGF cDNA序列，并去除5'端9 bp的序列，使N端缺少对肠激酶(Enterokinase， EK)具有抑制作用的脯氨酸，将截短型MGF des(1-3 MGF cDNA序列克隆入pET32a质粒，构建重组表达质粒。

The phenotype, calcium tuberculation and the expression of alkaline phosphatase were studied with alizarin red staining and modified Gomori Ca-Co assays respectively.

采用碱性磷酸酶Gomori 钙钴法染色、钙结节茜素红法染色及Ⅰ型胶原免疫组织化学染色方法进行鉴定成骨细胞生物学特性。

A 3 cm long segment was removed from the middle part of the rabbit radius to make a bone defect model. The periosteum was reserved and restored to set up a vascularized tubulate periosteum graft.

将兔双侧桡骨干中段切除 3cm制成骨长段缺损模型，保留切骨段骨膜，重新复原缝合后作带血运骨膜管移植模型。

The ionic dissolution products of wollastonite coating in culturing medium can activate gene express and phenotype differentiation of osteoblast.

硅灰石涂层在培养液中的溶解产物能激活成骨细胞的基因表达和表型分化。

To observe the effect of distraction osteogenesis of zygoma based on the bony structure and bony density of new bone formation.

通过新生骨骨质结构和骨密度分析观察犬颧骨牵张成骨效果。

Methods Serum containing achyranthes root was prepared, human mesenchymal stem cells were separated and amplified, MTT assay was used to access the effects of serum containing achyranthes root on human mesenchymal stem cells in vitro, alkaline phosphatase and osteocalcin were assayed to reflect the variations of stem cell differentiation, and the expression of osteogenic transcription factors with RT-PCR.

制备牛膝含药血清，分离与扩增人间充质干细胞，MTT法检测牛膝含药血清对体外培养的间充质干细胞增殖作用的影响，检测碱性磷酸酶与骨钙素以反映细胞分化水平的改变，RT-PCR检测成骨相关转录因子的表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。