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Four, 8, and 12 weeks after operation, all specimens were examined by X-ray and histological methods.

分别于术后4、8、12周采用X线和组织学方法检测缺损部位的成骨情况。

Methods:(1) Bone marrow stromal cells isolated from Beagle dogs were cultured in vitro for the primary culture,in basic medium consisted ofDMEM^iew horning cattleerum,etc. Parts of subculture were cultured in mineralization medium which additionally contained 50 y g/ml ascorbic acid, 10mmol/LNa- beta -Glycerophosphate and 10-8mol/L dexamethasone in order to induce cells differentiating into osteoblasts.

①抽取毕格犬髂骨骨髓体外分离培养获得骨髓基质细胞,DMEM、新生牛血清培养基进行原代培养,部分传代细胞以含10~(-8)mol/L地塞米松、50μg/ml抗坏血酸和10mmol/Lβ—甘油磷酸钠的矿化培养液诱导其向成骨细胞增殖分化,用倒置相差显微镜进行细胞形态学和细胞生长增殖观察。

Objective To study the effect of hypoxia on osteoblast proliferation and apoptosis.

目的 探讨缺氧对体外培养成骨细胞增殖及凋亡的影响。

OBJECTIVE: To investigate the effects of liquid nitrogen cryopreservation on osteoblast immunogenicity.

目的: 探讨液氮冷冻对成骨细胞免疫原性的影响。

Abstract] Objective:To study the effect of GMCSF and its inducing osteoclast on proliferation of osteoblast.

目的:探讨GMCSF和其诱导的破骨细胞对成骨细胞生长的影响。

ITS inductive system is effective in encouraging the maturity and terminal differentiation of chondrocytes.

ITS诱导体系有效地促进了软骨细胞成熟及终末分化,较成功模拟了软骨内成骨的软骨发育过程。

In recent years, some researches proved that TCM could induced MSCs differentiated into neuron cells and osteoblasts effectively and the inductivity was high.

近年来,有研究证明中药能有效地诱导MSCs分化为神经元细胞和成骨细胞,并且诱导率高。

Experimental study on the bone inductivity of rhBMP 2 collagen and coral complex[J].

重组人骨形成蛋白 2、胶原、珊瑚复合人工骨异位诱导成骨的实验研究[J]。

The biological activity glass has the good bone conduction, the inductivity, can accelerate osteocyte multiplication, causes the osteoblasts to transform into the mature osteocytes.

生物活性玻璃具有良好的骨传导性、诱导性,能加速骨细胞的增殖,使成骨细胞转变为成熟的骨细胞。

New bone ingrowth need three basic factors 1 osteogenic cells 2 osteoinductive agents 3 osteoconductive materials.

组织工程化骨应包括三个基本要素 1)骨前体细胞 2)诱导成骨因子 3)合适的生物材料载体。

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