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The modified method, floating explant culture, provides a useful and simple method for the studies of bone biology and bone tissue engineering.

组织块漂浮培养法是一种简便、可行的培养方法,能够为成骨细胞的相关研究提供稳定可靠的种子细胞来源。

Objective : To study the effect of fluoride on osteoblasts.

目的:探讨氟对成骨细胞的影响。

Objective: To observe the effects of sophoricoside and genistein on the biological characteristics of osteoblasts.

目的:比较槐角苷及染料木素对成骨细胞生物学特性的影响。

Calcic phosphor is the bases of form ossification road and tooth, both lacks fetal skeleton with respect to development undesirable, bring about gibbosity, the mother's body still can give view cramp, osteoporosis.

钙磷是形成骨路和牙齿的主要成分,二者缺乏胎儿骨骼就发育不良,导致软骨病,母体还会出观抽筋、骨质疏松。

Methods Bone metabolism markers were measured and their correlations with thyroid hormones were analyzed in 49 patients with Graves' disease.

Graves病患者在过量甲状腺激素作用下,成骨细胞和破骨细胞的活性和数量发生变化,导致骨代谢紊乱。

Total RAN was extracted from osteoblasts using guanidine, and mRNA expression of OPG and RANKL were examined using RT-PCR.

成骨细胞骨形成功能的减退和/或破骨细胞骨吸收作用的增强可造成代谢性骨病,如骨质疏松。

Although underlying mechanisms ⑴ hae not been elucidated, eidence points to the participation of the nitric oxide and cyclic guanosine 3',5'-monophosphate system.

虽然根本的机制还没有阐明,但是证据显示一氧化氮和环鸟苷酸系统参与其中。这里,我们检测到在MC3T3-E1成骨细胞的分化中基质金属蛋白酶13的mRNA,蛋白和活性的升高,诱生一氧化氮合酶和一氧化氮产物的升高。

We are interested in the relationship between the activity of osteogenesis and hematopoiesis of OGP and Bone Marrow Mesenchymal Stem Cells.

骨髓间充质干细胞是骨髓中一类具有多向分化潜能的干细胞,是骨髓造血微环境的重要组成部分,在适当的条件下可以分化为成骨细胞、软骨细胞、脂肪细胞等。

According to the bone apposition events at the interface, we measured the osteoblast attachment rate with the hemocytometer, the osteoblast growth curve with modified MTT method, the ALPase activity and protein content of cellular layers with modified kinetic method and modified Coomassie'method respectively, and osteocalain content in the cell medium with RIA , at different levels of cell responses, as osteocompatibility indexs.

根据骨内种植体界面的骨愈合过程,分别从成骨细胞反应的不同层次选择了细胞贴壁率、细胞生长曲线、细胞层ALPase活性和蛋白质含量以及细胞培养液中骨钙素的含量作为体外评价材料骨性生物相容性的指标。采用细胞计数板计数法测定细胞贴壁率,采用改良MTT法测定细胞生长曲线。

The research of the characteristics of biology and the osteogenic and adopocitic induction are comparatively mature, showing many biological characteristics such as adherence, plasticity, heterology, self-renewal, rapid clone formation and transplantation. Adherence combined with density gradient centrifugation method is widely used in the isolation and purification of BMSCs.

骨髓间充质干细胞现阶段研究方向主要包括生物学特性、诱导分化和调控机制等内容,对其生物学特性和成骨成脂诱导方面研究比较成熟,在培养分化过程中展示出贴壁性、可塑性、异质性、自我更新能力、快速形成克隆能力、可移植性等生物学特征,贴壁法联合密度梯度离心法在骨髓间充质干细胞的分离、纯化过程中被广泛采用。

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