成骨

In the process of osseo-integration, cellular attachment to the material is the premise for cellular vital activity, such as proliferation, differentiation and apposite et al.

在此过程中，成骨细胞粘附到种植材料表面是成骨细胞发挥其功能的先决条件，成骨细胞在金属钛表面上的粘附对骨结合界面的形成是至关重要的。

During a special biomineralization process—shell regeneration, we found that both CaLP exhibit special functions in shell related secretory vesicles; during the differentiation of osteoblast MC3T3-E1, CaLP exhibite significant effect on its differentiation and biomineralization.

在对切壳修复这一特殊的生物矿化过程的研究中发现CaLP积极参与了新生贝壳的形成；在前成骨细胞MC3T3-E1向成骨细胞分化的过程中，CaLP能够促进成骨细胞的分化和矿化过程。

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。方法：取新西兰白兔骨髓液经离心后得骨髓单个核细胞，以1× 106／ml的细胞浓度进行培养，经传代培养，选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol／l，β-甘油磷酸钠10mmol／l，VitC 50μg／ml)作为骨髓基质细胞增殖和成骨的调节因子，通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase，ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

Results The BMS absorbing bone marrow cell has the bestfunction of new bone formation in the 12th week.The second is HA,the cuttle bone haslittle and gelfoam is unable to formate new bone.

结果 12周时，骨基质海绵组成骨能力最强，羟基磷灰石组次之，海螵蛸组成骨能力最弱，明胶海绵组无成骨能力。

Methods We isolated and cultured the MSCs, then induced MSCs into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene (collagenase col Ⅰ) col Ⅰ and ALP expression was detected.

使用诱导方法作用于骨髓基质干细胞，使之转化为成骨细胞；用RT-PCR检验成骨细胞；不同浓度的二磷酸盐或骨碎补总黄酮配制成条件培养基，作用于成骨细胞，并分析生长曲线，分析细胞基因碱性磷酸酶和胶原酶Ⅰ(collagenase col Ⅰ)表达情况。

Human BMSCs after being induced have the strong osteogenic ability, and the compounds of decell decalcification spongy bone human BMSCs osteoblast is a kind of ideal ossification of tissue engineering.

人BMSCs诱导的成骨细胞具有良好的体内成骨能力，脱细胞脱钙骨松质人BMSCs 成骨细胞是较理想的组织工程化骨。

The immunohistochemistry results indicate that TGF-β1 plays an important role in the distraction osteogenesis.We expect that we can utilize the ectogenesis TGF-β1 to accelerate bone formation in the near future.

有望利用外源性TGF-β1加速牵张成骨中的骨形成，并提高形成骨的质和量，从而为牵张成骨术更好地应用于临床提供理论基础。

In previous studies, Cyclooxygenase-2(COX-2) is believed to be linked with inflammations and tumors. But in recent reports, COX-2 is found to play important roles in osteoblastic mechanotransduction and in bone remodification and resorptions in some ways. It was found that mechanical stretches, such as hydrostatic forces and pulsating fluid flow, could increase the expressions of COX-2 mRNA and then prostaglandins, and activate many signal pathways in osteoblasts.

环氧合酶-2(cyclooxygenase-2，COX-2)以往被认为与炎症、肿瘤等有密切关系，近年研究发现它在骨骼重建、骨质吸收以及成骨细胞力学信号转导中发挥着重要作用，主要表现为机械牵拉、流体力、节律性液体流(pulsating fluid flow，PFF)等机械刺激均能引起成骨细胞COX-2高水平表达，进而引起前列腺素(prostaglandins，PGs)量的增加，激活成骨细胞内多条信号传导通路。

METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

采用全骨髓法体外分离培养人骨髓间充质干细胞，传至第3代以1×108 L-1密度接种至预置盖玻片的6孔培养板中，设立3组：成骨诱导组添加原代培养液后，再加入含10-8 mol/L地塞米松、10 mmol/L β-磷酸甘油、50 mg/L维生素C的成骨诱导培养基；成骨+瘦素诱导组添加成骨诱导培养基后，再加入50 nmol/L瘦素；空白对照组仅加入含体积分数为10%胎牛血清的LG-DMEM培养液。

The change of cytoskeletal architecture of osteoblasts with Phalloidin strain actin filaments was visualized by confocal microscopy The results showed: 1 the secretion of estrogen were depressed and bone mass were decressed and biomechanic properties of bone were impaired in osteoporotic rats at 3 months after ovariectomy .

在1 000μstrain力学刺激下，成骨细胞integrinα2，β1，β3的表达量出现上调；在4 000μstrain力学刺激下，成骨细胞integrinα2，β1，β3的表达量明显上调，成骨细胞合成骨基质的能力与增殖活性增加。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐