成骨

Excellent osteogenous and degrade property on the surface UMCHA.

证实UMCHA骨水泥表面培养成骨细胞具有较高的成骨活性

Includes growth factors including bone morphogenetic protein from the solidification calcium phosphate, has induction osteogenous energy, may sharpen the bone ability, is suitable for the marrow compound transplant for nonnegative heavy or carries a heavy load lowly the area tumorous bone damage repair.

自固化磷酸钙含有骨形态发生蛋白等生长因子，具有诱导成骨性能，可提高成骨能力，与骨髓复合移植适用于非负重或低负重区肿瘤性骨缺损的修复。

The biological features of hBMSCs and cryogentic storaged cells were analyzed.hBMSCs memberane antigens were identified by immunofluorescence staining.The potential osteogenous differentiation of hBMSCs was evaluated by the osteogeneic inducer kit.

倒置光学显微镜下观察细胞形态及生长情况，细胞计数法绘制细胞生长曲线，并分析冻存细胞复苏后的生长特性，免疫荧光染色测定hBMSCs表面标志，成骨诱导试剂盒检测hBMSCs向成骨分化的潜能。

Cell growth curve was also drawed before and after differentiation. When cultured in the osteoplastic induction medium, ADSCs derived epithelial cells might further differentiate into osteoblasts.

在成骨细胞诱导培养基中进一步诱导ADSCs源表皮细胞向成骨细胞定向分化，对诱导后的细胞进行ALP染色和Yon Kossa染色检查。

Objective: The purpose of this study is to establish a periosteal distraction model on rabbit mandiblar ramus, to observe the formation of newly formed bone and its structure in each phase after distraction of periostea, to observe the changes of periostea after being secondary to distraction forces, to investigate the possibility of osteogenesis by periosteal distraction in oral and maxillofacial region, and to study the possibility of a new restoration method of clincal alveolar and other bony defect.

目的：本研究在兔下颌升支建立骨膜牵张成骨模型，观察牵张骨膜后，在骨膜下新骨的形成，以及新骨在各个阶段的结构，同时观察了骨膜受到牵张力后的改变，研究骨膜牵张在口腔颌面部成骨的可能，为牙槽骨缺损及其他口腔颌面部骨组织缺损探讨新的重建方法。

The periostea of both experimental and control side of the mandibular ramus were taken and prepared, 2 of each 5 rabbits in a group were prepared for HE stain detection and 3 for proliferating cell nuclear antigen immunohistochemical detection.Results:1, The newly formed bone was detected on the lateral aspect of mandibular ramus after periosteal distraction. The bone was shaped like a hill. It looked very low and was full of holes at postoperative day 28. With the time of consolidation period lengthened, the newly formed bone matured gradually. X-ray examination showed the new bone shaped like a hill. The average values of new bone height at postoperative days 28,35,42 and 56 were 1.86 + 0.15mm, 2.29 + 0.29mm,3.19 + 0.13mm and 4.70 + 0.45mm. Histological examination of both HE stain and picricacid-fuchsin stain showed the increase in the number of osteoblasts and the change in the orientation of collagen fibers and bone trabecula. There were no significant differences between newly formed bone and original bone on the lateral aspect of mandibular ramus at postoperative day 56 histologically.2 Compared with the control side, the distracted periostea proliferated obviously under the microscope, and the number of periostealcells increased with satiation of cellular nuclear per unit area. The images of PCNA immunohistochemical stain of periosteum showed that the experimental periosteum proliferated obviously after distraction compared with the control side.

结果：骨膜牵张成骨的实验研究南京医科大学硕{学位论文l、骨膜牵张后，可见下领升支外侧的骨皮质上有新骨形成，新骨呈山峰状凸起，术后第28天的新生骨较低平，多孔隙，随着固定时间的延长，新骨逐渐成熟；下领升支前后向切线位X线投照显示新骨呈山峰样隆起；经测量，术后第28、35、42和56天组平均新生骨厚度分别为x.86士0.15mm、2.29士0.29mm、3.19士0.13mm和4.70 土0.45mm；脱钙骨组织的HE染色和不脱钙骨组织的苦味酸一品红染色的组织学观察均显示了新生骨在成骨细胞数量上的增长，以及胶原纤维和骨小梁排列方向上的变化，术后第56天的新生骨在组织学上与原升支骨组织已无明显区别。2、HE染色显示，与对照组相比较，实验侧骨膜增生明显，细胞间排列紧密，单位面积内骨膜细胞数增多，细胞核饱满；骨膜PCNA 免疫组化染色显示，与对照侧相比较，实验侧骨膜在牵张后出现了明显的增生迹象，PCNA阳性细胞分布紧密，单位面积内阳性细胞数较对照组多，靠近骨表面的骨膜中的阳性细胞数更多而且分布更为紧密。

OBJECTIVE: To investigate isolated culture and conservation method of human periosteal osteoblasts, in addition, to observe the biological characteristics.

目的：建立人胚胎骨骨膜来源成骨细胞的分离培养、保存方法，观察骨膜来源成骨细胞生物学特点。

Objective To investigate the proliferative and osteogenic activity of periosteal osteoblasts, calvarial osteoblasts and bone marrow stromal cells from the same embryo.

目的 比较从同一胚胎来源的骨膜成骨细胞、颅骨成骨细胞和骨髓基质干细胞的分裂增殖能力和细胞的功能状态。

Methods Periosteal cells were isolated and cultured in vitro and then exposed to dexamethasone 10 -8 mol/L, 10 -7 mol/L an...

利用多种细胞因子促进细胞向成骨细胞的分化，以保证细胞在植入体内后能够保持并充分发挥其成骨活

Results There existed action windows of amplitude and frequency with regard to the biological effect of recta...

结论ELFEMF对成骨细胞的影响依赖于电磁场的特征参数，对成骨细胞的增殖与分化而言，磁感应强度5mT、频率15Hz、占空比15%可能为一组合适的参数。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。