成骨

Bright pink gelatiniform liquid with mass ratio of sodium alginate and gelatin of 2:3 was prepared. Rabbit osteoblasts at 5×109/L were mixed with CaCl2 solution to form fruit jelly-shaped sodium alginate-gelatin/osteoblast gel. Critical-sized calvarial defects were created in diameter of 1.5 cm in 15 rabbits. After 1 week, cell/scaffold complex (0.5 mL) was implanted.

制备海藻酸钠与明胶质量比为2∶3的透明粉红色胶状液体，引入兔成骨细胞，细胞终密度为5×109 L-1，与CaCl2溶液混合，形成果冻样海藻酸钠-明胶共混体系/成骨细胞凝胶。15只兔颅骨均制备直径1.5 cm的极限缺损，1周后植入凝胶复合体0.5 mL进行修复。

Bright pink gelatiniform liquid with mass ratio of sodium alginate and gelatin at ratio of 2:3 was prepared. Rabbit osteoblasts with final concentration of 5×109/L were mixed with CaCl2 solution to form fruit jelly-shaped sodium alginate-gelatin/osteoblast gel. Critical-sized calvarial defects were created in diameter of 1.5 cm in 12 rabbits. After 1 week, cell/scaffold complex (0.5 mL) was implanted to repair the bone defect in the experimental group. There was no treatment in the control group.

制备海藻酸钠与明胶质量比为2∶3的透明粉红色胶状液体，引入兔成骨细胞，细胞终浓度为5×109 L-1，与CaCl2溶液混合，形成果冻样海藻酸钠-明胶共混体系/成骨细胞凝胶。12只兔颅骨均制备直径1.5 cm的极限缺损，1周后实验组植入凝胶复合体0.5 mL进行修复，对照组不植入任何材料。

Differential expression of IGF-I in muscles of mastication during distraction osteogenesis of mandible in canine.This study was conducted to observe the expression of IGF-I in masseter and geniohyoid during distraction osteogenesis of mandible in canine through the immuno-histocytochemical method.

试验三犬下颌骨牵张成骨过程中咀嚼肌IGF.I的表达变化将所取标本进行免疫组化染色，检测犬下颌骨牵张成骨过程中嚼肌与颁舌骨肌中IGF.I表达的变化。

In this experiment, in the way of observing the histological changes, relative content of myosin heavy-chain mRNA and changes of the expression of IGF-I in masseter and geniohyoid muscle during distraction osteogenesis of canines mandible, we can learn about the rebuilding ability of masticatorymuscles and its changing regularity, and preliminarily discuss its remodeling mechanism.Anteroposterior bilateral distraction osteogenesis was performed on 14 mongrel canines, 6 to 8 months of age, using intraoral mandibular distractor.

本实验通过观察犬下颌骨牵张成骨过程中嚼肌与颏舌骨肌的组织学变化、肌球蛋白重链亚型mRNA含量以及IGF-I在咀嚼肌中表达的变化，了解牵张成骨过程中口颌肌肉的改建能力及其变化规律并初步探讨其改建机制。

Cells in the induction group were incubated in 1.5 mL of osteoinductive medium, supplemented with 10-8 mol/L dexamethasone, 10 mol/L β-glycerophosphoric acid, and 50 mg/L vitamin C.

选取第3代骨髓间充质干细胞和脂肪间充质干细胞，按5×107 L-1接种后各分为2组：诱导组加入含10-8 mol/L地塞米松、10 mol/L β-甘油磷酸、50 mg/L维生素C的成骨诱导培养基1.5 mL，未诱导组不加入成骨诱导培养基。

METHODS: The third passage of BMSCs and AMSCs at a density of 5×10^7 L^(-1) were divided into two groups. Cells in the induction group were incubated in 1.5 mL of osteoinductive medium, supplemented with 10^8 mol/L dexamethasone, 10 mol/L β-glycerophosphoric acid, and 50 mg/L vitamin C. Cells in the non-induction group were not treated with osteoinductive medium.

选取第3代骨髓间充质干细胞和脂肪间充质干细胞，按5×10^7L^(-1)接种后各分为2组：诱导组加入含10^8mol/L地塞米松、10mol/L β-甘油磷酸、50mg/L维生素C的成骨诱导培养基1.5mL，未诱导组不加入成骨诱导培养基。

Detect that whether the ADSCs were differentiated inducedly into osteoblasts or adipogenic cells by Alkaline phosphatase staining、Von kossa staining and Oil red "O"staining after the ADSCs were cultured in inductive basic medium containing osteogenic induction agent and adipogenic inducers for four weeks.②.

①。采用密度梯度离心法加贴壁培养法对兔脂肪组织进行了分离纯化，CD44免疫组化法分析ADSCs表面标志；用含成骨、成脂诱导培养基培养4周后，行碱性磷酸酶染色、Von kossa染色及油红&O&染色检查ADSCs是否向成骨、成脂细胞定向分化。②。

In intramembranous ossification, absence of cartilage, osteoblasts are likely producing, and responding to, VEGF.

膜内成骨中，在无软骨出现的情况下，成骨细胞产生、应答VEGF。

RESULTS ① rhBMP2/BCB used alone was capable of healing the defect in large part by 16 wk, with a similar repair process and mechanism to that seen with RBX;② use of rhBMP2/BCB in conjunction with vascularized periosteal graft exhibited stronger defect-repairing power, basically healing the defect by 8 wk. The repair process consisted of intramembranous and endochondral ossification that resembled the physiological repair during the fracture healing.

结果①单纯rhBMP2/BCB移植，可在16 wk使节段性骨缺损基本修复，其机制与过程与重组合异种骨相似；②rhBMP2/BCB与带血循骨膜联合移植，仅用8 wk即可修复骨缺损，其修复机制与骨折修复相仿，包括膜内成骨和软骨成骨两种机制。

L To observe the expression rule of miRNA when the lipocyte stem cells differentiated into cartilage cells or bone cells in different micro-environment and stages by in vivo tests and in vitro study.

通过体内、外试验，观察不同环境、不同阶段家猪脂肪干细胞向软骨、成骨方向分化时miRNA表达的规律。（3）研究双层一体Collagen/TCP影响家猪脂肪干细胞 miRNA表达的变化，并验证与鉴定成软骨、成骨分化相关的miRNA。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐