成釉

It is suggested that the stratum intermedium participate in the differentiation of ameloblast and formation of enamel.

中间层细胞的增殖和凋亡及其TNSAP的表达与成釉细胞具有相关性，提示中间层细胞有可能参与成釉细胞的分化及釉质形成。

Results The expression of Bag-1 protein both in ameloblast and odontoblast was inhibited in experimental group.

结果 实验组大鼠切牙釉基质形成紊乱，成釉细胞和成牙本质细胞Bal-1蛋白表达明显低于对照组，差异有显著性(P.01)。

Immunohistochemical staining during different stages in ameloblast odontoblast and enamel free area cells were measured by the image analysis,the date of which was handled with SPSS11.5 software.

随后做图像分析测定不同发育阶段的成釉细胞、成牙本质细胞、无釉区细胞的免疫表达水平，所得数据用SPSS11.5 软件进行统计分析。

There is a significant linear correlation between stratum intermedium cell and ameloblast in apoptosis and expression of PCNA and TNSALP.

中间层细胞先于成釉细胞表达TNSALP，出生后5d时即呈现强阳性染色，而后其表达又逐步减弱；成釉细胞7d时开始出现阳性，且呈逐渐增强趋势。

Results: Ameloblastin mRNA was negative under microscope in the ameloblast proliferate differentiation period, it showed weakly positive expression in intra-cellular and new-birth enamel matrix in secrete initial stage while it showed strongly positive expression in intra-cellular and new-birth enamel matrix when it came to secretion phase.

结果：釉鞘蛋白mRNA在成釉细胞增殖分化期表达为阴性，分泌初期细胞内及新生釉基质中开始出现弱阳性表达；至分泌期，细胞内及新生釉基质中均呈强阳性表达。

RESULTS: CaB was not detected during early odontogenesis from the dental lamina to the late c ap stage .Small amount of the protein first appeared in ameloblast and some of dif ferenting cells in the surface of dental papilla in early bell stage. As to late bell stage, the cytoplasm of ameloblasts and odontoblasts with secreted dentin was all stained strongly, as well as some of n uclei.

结果：人牙胚蕾、帽状期成釉器无CaB的表达；钟状早期成釉器的内釉细胞及部分牙乳头表面处于分化状态的细胞弱阳性染色；钟状晚期成釉器的成釉细胞、成牙本质细胞及部分胞核呈现强阳性染色。

Results showed that β-catenin was positive expression in stellate reticulum,inner dental epithelium,and dental sac at cup stage,in inner dental epithelium,dental papilla cell,and cell nucleus at early bell stage,in cervical loop and cusp cell membrane at late bell stage,and in ameloblast and odontoblast cell at amelogenesis stage.

结果表明，帽状期在星网层、内釉上皮、牙囊均为细胞核阳性表达；钟状早期在内釉上皮、牙乳头细胞核阳性表达；钟状晚期在颈环、牙尖细胞膜阳性表达；成釉期早期在成釉质细胞、成牙本质细胞阳性表达；成釉期晚期均阴性表达。

To study the characteristics of the mesenchymal cells of ameloblastic fibrosarcoma, three cases of AFS were studied immunohistochemically and ultrastructurally.

为探讨成釉细胞纤维肉瘤间叶细胞的本质及特征，作者采用免疫组化和电镜技术，对3例AFS间叶细胞进行了研究，并与6例成釉细胞纤维瘤进行比较。

The ameloblasts are the key cells during odontogenesis, and they have been already cultured in vitro. The amelogenin is the extracellular matrix synthesized and secreted by the ameloblasts, and it plays a crucial role during amelogenesis.

成釉细胞是牙器官形成的关键细胞，国内外已成功离体培养出成釉细胞，成釉细胞合成和分泌的细胞外基质－釉原蛋白在釉质的形成中起着关键作用。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。