成酶的

Alkaline lipase Greasex 50L is one of the leather enzyme products of Novo Nordisk AS. After extensive control trials on pigskins, evaluating effects by degree of fat removal and qualities of result leather, we got1 Fat removal rate can be increased by 10%and leather qualities such as napping and softness can be improved by using Greasex 50L together with liming enzyme NUE 0.6 MPX2 Some surfactant together with Greasex 50L is good to fat removal, and the recommended ratio is 0.05%～0.1%∶13 The fat content in the skins is lower after treated with Greasex 50L,which also benefits the successive processes, such as bating, pickling and tanning. As a result, the leather qualities, such as fineness of grain, softness, napping and evenness of dyeing can be improved.

Greasex 50L是诺和诺德公司生产的碱性脂肪酶已在皮革工业中广泛使用，为了更好地应用这种酶制剂，我们对Greasex 50L在猪皮方面的应用条件进行了研究采用对比的方法，用脂肪去除率和皮革的质量作为评价标准，研究结果表明1适量的浸灰酶 NUE 0.6 MPX可使Greasex 50L的脱脂率增大，同时可改善皮革的起绒状况和革身柔软性2一定量的表面活性剂与Greasex 50L配合使用，有利于脂肪的去除，用量为平平加的0.05%～0.1%3 经Greasex 50L脱脂的裸皮在鞣制后油脂含量也较低，有利于后续工序进行，从而使成革粒面平细、革身柔软，臀部起绒得到改善，染色均匀性良好

All of those confirmed the odontogenetic ability of adipose-derived stem cells, offered theory and experimental basis for the abundant seed cells in tooth tissue engineering, construct new research model for the micro-environment of the odontogenesis in vitro and vivo.

目的 本研究通过酶消化法分离培养脂肪干细胞，运用牙胚细胞条件培养液体外诱导脂肪干细胞向成牙本质样细胞分化，再将脂肪干细胞与成釉器上皮细胞混合接种入体内建立牙髓-牙本质复合体模型，证实大鼠脂肪干细胞的成牙潜能，为牙齿组织工程化研究获得大量、稳定、可靠的种子细胞提供理论和实验依据；并为体内、外成牙微环境的机理研究构建新的模型。

The reverse transcription-polymerase chain reaction was used to seek the expressions of a specific fibroblast marker, prolyl 4-hydroxylase β, and a specific marker of epithelial cells, cytokeratin-4, in the cells.

采用逆转录多聚酶链反应检测hEFs表达成纤维细胞特异性标志（脯氨酰4-羟化酶β亚单位）和上皮细胞特异性标志（细胞角蛋白4）的情况，对该细胞进行鉴定。

The first passage cells were initially pured by enzyme digestion, then pured by enzyme digestion and repeated strapping alternatively in the second course of transferation and inoculation, the third period cultured cells showed typical clonali growth and were long fusiform fibroid cells with short growth cycle.

首次传代时应用胰酶消化法进行成纤维细胞的初次纯化，第2次转种时先后应用酶消化法和反复贴壁法再次进行细胞纯化，第3代以后基本可获得呈典型克隆性生长、长梭形纤维样细胞，生长周期短。

Under the optimum conditions (i.e., reaction at 40℃ using hyperthermophilice esterase APE1547, 4-Cl-benzaldehyde substrate, and trichloromethane solvent), this reaction provided optically active secondary alcohol with an enantiomeric excess up to 56% and a yield up to 78%.

在最适条件（嗜热酯酶APE1547为酶源，反应温度40℃，氯仿为溶剂，4-Cl-苯甲醛为底物）下，加成反应生成的光学活性醇产率最高达78%，ee值最高可达56%。

The results show the Boer goat oocyte and pre-implantation embryo are telomerase positive. According to the densitometry of bands under the CCD imagine system, the total telomere repeats product generated of oocytes, 4-cell, 8-cell, morulae and blastocyst are 25.348, 273.832, 56.117, 251.118, 519.46, respectively.

结果表明波尔山羊卵母细胞和早期胚胎都是端粒酶阳性；依据电泳条带在凝胶成像系统下的光密度计算相对总产品产量定量比较发现，卵母细胞最低为25.348，4-细胞为273.832，至8-细胞又降低到56.117，随后快速升高，桑椹胚为251.111，囊胚端粒酶活性最高为519.46。

RESULTS: Stable telomerase activity could be detected in the transfected fibroblasts and no signs of cell senescence were found in the fibroblasts cultured for more than 50 doublings.

结果 转染 h TERT的人成纤维细胞能稳定表达端粒酶活性，培养超过 5 0代后细胞仍保持β-半乳糖苷酶阴性。

S-TNF, TM-TNF and S-TNFm were then in vitro translated and synthesized in the presence or absence of microsomes.

进一步酶切分析表明TM-TNF是通过某些金属蛋白酶酶解作用而被转换成S-TNF的。

Unilever and others have produced lipase to process palm oil to produce cocoa butter- like fat, and enzymes have also been used in the production of semi-synthetic penicillin.

Unilever公司已经生产出脂肪酶，用来把棕榈油加工成可可奶油脂肪。酶还用在生产半合成的青霉素上。

An inactive form of plasmin that occurs in plasma and is converted to plasmin by organic solvents.

血浆中血纤维蛋白溶酶不活跃的前体，能被有机溶剂转化成血纤维蛋白溶酶。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。