成酶的

Methods We isolated and cultured the MSCs, then induced MSCs into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene (collagenase col Ⅰ) col Ⅰ and ALP expression was detected.

使用诱导方法作用于骨髓基质干细胞，使之转化为成骨细胞；用RT-PCR检验成骨细胞；不同浓度的二磷酸盐或骨碎补总黄酮配制成条件培养基，作用于成骨细胞，并分析生长曲线，分析细胞基因碱性磷酸酶和胶原酶Ⅰ(collagenase col Ⅰ)表达情况。

High expression of hTERT mRNA in the fibroblasts of keloid may be related to the telomerase activation.

推测人端粒酶催化亚单位mRNA在瘢痕疙瘩成纤维细胞中的高表达与端粒酶活性密切相关。

The results of analysis on LDH isoenzyme spectrum showed that there were 2 spectrums in Beijing Fatty Chicken and Langshan Chicken, and 5 spectrums in Small-tailed Han Sheep. The result of analysis on MDH isoenzyme spectrum shows that there were 2 spectrums in all the three breeds, but mobility of Small-tailed Han Sheep was significant higher than the 2 poultry breeds.

LDH同工酶谱带分析表明，北京油鸡、狼山鸡成纤维细胞为两条谱带，小尾寒羊为5条谱带：MDH同工酶谱带分析三种品种均为两条谱带，但小尾寒羊的迁移率明显高于北京油鸡、狼山鸡。

Diketoreductaseis a class of new enzyme, and compared with other oxidoreductase, whose uniqueness lies in its ability to restore at the same time reduced to two hydroxy carbonyl and a high degree of stereoselectivity.

双羰基还原酶（Diketoreductase，简称DKR）是一类全新的酶，与其他的氧化还原酶相比，其独特之处在于它能够同时还原两个羰基还原成羟基，并有高度的立体选择性。

The pancreatic juice also contains three main enzymes which tear proteins, fats and carbonhydrates apart into basic building blocks.

胰液内还含有三种主要的酶，这些酶能把蛋白质、脂肪和碳水化合物分解成基础建筑材料。

Results hPDLP wasisolated from human periodontium and most of the cells retained their fibroblastic spindle shape. hPDLP can be in-duced into osteoblast-like cells and adipocyte-like cells, and calcium deposition and lipid droplets were detected perspectively.

结果 第1代hPDLP的CD146和STRO-1阳性率分别是27.20%±3.98%和4.23%±4.08%；经矿化诱导可以形成矿化结节，有钙盐沉积；经成脂诱导可见特异性脂滴形成，特异性转录因子过氧化物酶体激活物增生受体2（PPARγ2）和脂蛋白脂酶表达上调。

Hyaluronidase can degrade hyaluronic acid into disaccharide compounds and dissociate intercellular substance berrier to liquefy the vitreous. Hyaluronidase is one of the most potential enzymes to assist vitrectomy.

透明质酸酶可使透明质酸水解成双糖化合物，并离解细胞间质屏障，使玻璃体液化，是目前辅助玻璃体切割手术最有应用潜力的酶之一。

When a catalytically active enzyme forms a complex with a cofactor a holoenzyme is produced.

酶蛋白和辅助因子两者结合成完整分子时，形成具有活力的全酶。

The results indicate that Ca(superscript 2+) and GA3, single or combined application treatments, promote cucumber seed germination treated with P. harmala alkaloid, increase germination rate, germination vigor and index; as well as α-amylase, protease, lipase, isocitratelyase, nicotinamide adenine dinucleotide kinase and Ca(superscript 2+)-ATPase activities; and GA and CaM content in cucumber seeds; but decrease abscisic acid content. The synergistic effects of combined Ca(superscript 2+) and GA3 on increasing GA and decreasing ABA are oberseved compared with Ca(superscript 2+) treatment.

结果表明，Ca(上标 2+)和GA3单一及复合处理可促进骆驼蓬生物碱处理的黄瓜种子的萌发，使黄瓜萌发率、发芽指数和活力指数提高，萌发种子的α-淀粉酶、蛋白酶、脂肪酶和异柠檬酸裂解酶活性及赤霉素和钙调素含量增加，烟酰胺腺嘌呤二核苷酸激酶和Ca(上标 2+)-ATP酶活性增强，脱落酸含量降低；且Ca(上标 2+)和GA3复合处理对GA含量的提高和ABA含量的降低与Ca(上标 2+)单一处理比较呈显著的加成作用。

The results suggest it be possible to establish the recombination bacteria of Nitrosomonas to oxidize the ammonia in wastewater.

第二步 ，由亚硝酸盐氧化成硝酸盐；其中第一步较慢，是限制性步骤，通过亚硝酸细菌完成，而在此过程中直接起作用的酶是氨单加氧酶(ammoniummonooxygenase，AMO)。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。