成酶的

In feed add glucanase, xylanase, cellulose-based compound enzyme enzymes, anti-nutritional factors can degrade into small molecules fragments, the release of nutrients, reduce chyme viscosity, so that nutrition the material can be fully digested and absorbed to reduce the ileum bacteria substrate, inhibiting growth and reproduction of harmful micro-organisms to prevent diarrhea.

在饲料中添加以-葡聚糖酶、木聚糖酶、纤维素酶等为主的复合酶制剂，能够将抗营养因子降解成小分子片段，释放营养物质，降低食糜粘度，使营养物质能被充分消化吸收，减少回肠中细菌的底物，抑制有害微生物生长繁殖，防止腹泻。

The flavor characteristics change of chicken bone hydrolysates with and without thermal inactivation was evaluated by paired comparison test and nine-point hedonic scale and identified by GC-MS.

采用差别成对比较和评分法评价了灭酶前后鸡骨风味的变化，采用GC-MS联用仪鉴定了灭酶前后鸡骨风味成分的差异，分析研究了鸡骨酶解过程中加热灭酶对产物风味的影响。

The test was conducted to study the influence of the different nitrogen concentration in float system on some agronomical and physiological characters of flue-cured tobacco plant at transplanting and 30 days after transplanting respectively.

以红花大金元烤烟品种为材料，研究了育苗阶段不同氮浓度营养液即50、150、250、350、450、550mg/kg对成苗时烟苗农艺性状、根系活力、脯氨酸含量以及移栽后30d时体内多酚氧化酶、过氧化物酶、过氧化氢酶等活性的影响。

Enzymes of glycolysis (hexokinase,lactate dehydrogenase,aldolase) showed low activities during overwintering when more carbon flowing into treh alose synthesis.

越冬期间体内糖原磷酸化酶活性明显地增加，糖酵解有关的酶（己糖激酶、乳酸脱氢酶和醛缩酶）活性较低，以保证更多的碳源转化成海藻糖。

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分：α—(2，3)／(2，6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平：但Cx43连接斑形成增多；2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合；3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成；4、唾液酸酶作用后细胞内ERK1／2磷酸化水平明显增加，细胞间同质粘附增加，以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制，酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力，酶蛋白亚基间的相互作用，酶蛋白的折叠以及内运动状态与酶活力的关系，酶蛋白发挥功能时的结构特性；运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性，热稳定性机制；确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位；确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构；确定了一个新型的染色质蛋白Cren7的溶液三维结构；正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

Expression of Bilirubin Oxidase cDNA from Myrothecium Verrucaria in E. coliThe 1. 6kb bilirubin oxidase cDNA from Myrothecium Verrucaria was excised from plasmid pGEM-T/BOX, and inserted into expression vector, pET-3a which were under the control of a T7 promoter.

用限制性内切酶将1.6kb疣疱漆斑菌胆红素氧化酶cDNA从质粒pGEM-T／BOX切下，插入到表达质粒pET-3a的T7启动子下游，构建成疣疱漆斑菌胆红素氧化酶表达质粒pET-3a／BOX。

The results show that the suitable conditions are enzyme concentration 5mg/mL, my-celium age 24h, zymolytic temperature 37 ℃, time 1h. The protoplast formation rate is 100%, The regeneration rate is 64.6%.

结果表明：出芽短梗霉原生质体制备所需的最佳条件是酶浓度5 mg/mL、菌龄24 h、酶解温度37℃、酶解时间1 h，在此条件下原生质体形成率达100%，再生率为64.6%。

The fact that urokinase can hydrolyse plasminogen to generate angiostatin without the participation of free sulfhydryl donor L-Cys suggests that there might be another pathway of angiostatin generation, in which urokinase hydrolyse plasminogen to generate angiostatin directly.

我们还比较了pH7.4、pH8.0、pH9.0三种pH条件下尿激酶酶切纤溶酶原生成血管抑素的反应结果，发现酶切活性在pH9.0时速度最大，pH8.0时反应速度最慢，pH7.4时反应速度介于二者之间，由此表明pH对上述酶切反应速度确实存在影响。

Five expressed genes contributed to the flowering process and embryonic development such as prolamine, gene for allergenic protein, chalcone synthase, putative peptide methionine sulfoxide reductase and acyl carrier protein Ⅱ gene were chosen to hybridize with a 1510-RNA-blot (time-point phenotypes) array. The 1510 RNA blots were prepared into an RNA array for the purpose of conferring and validating global transcriptional profiles on booting, flowering and filling quantitatively and qualitatively at a statistic level. The results verified: Prolamine and allergenic protein were high-expressed in filling grains after being regulated by development, Chalcone synthase and methionine sulfoxide reductase were high-expressed in the leavies induced by light, in the root stressed for nitrogen deficiency.

为了全局性分析基因表达谱以及进一步验证它们与开花过程的相关性，选取参与水稻开花过程和胚胎发育过程的基因：查尔酮合酶、酰基载体蛋白Ⅱ、醇溶蛋白、S-腺苷基甲硫氨酸还原酶、过敏反应蛋白等为探针，与1500个水稻RNA斑点阵列进行杂交时，结果证实：醇溶蛋白和过敏反应蛋白受发育调节在乳熟成穗中高表达，查尔酮合酶和S-腺苷基甲硫氨酸还原酶在叶片中受光诱导、在根中受缺氮胁迫高表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。