成酶的

The sex pheromone-producing gland of Ancylis.sativa Liu is amodified intersegmental membrane as a dorsal bag between eighth and ninthabdominal segments.

对枣粘虫雌蛾性信息素腺体的扫描和透射电镜观察表明，枣粘虫性信息素腺体是由第八、九腹节间的节间膜特化而成，是位于背部的一个囊状结构，它由两部分构成，前部的方形囊状体和后部的三角形囊状体，二者之间有一突起的脊；雌蛾静止时，腺体随第八和第九腹节一起嵌缩于第七腹节内，求偶时，腹部末端外伸，腺体细胞表皮外露，释放性信息素；腺体细胞呈方形，其表面覆盖几丁质表皮，表皮顶端无孔；细胞核很大，呈椭圆形，由双层核膜包被，位于细胞中下部，内有丰富的染色质；细胞质内有丰富的内质网，其内还有大量的大小不等的脂肪滴，以及线粒体和溶酶体等细胞器。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Study the bioactivity of the n-HA/PA66 composite and the effects it would be to body's metabolism of calcium and phosphorus ion in vivo.(3) Study the osteo-conductivity and the ability to repair bone defect of the porous n-HA/PA66 composite and the feasibility use it as the scaffold of bone tissue engineering. Objects and Methods as follows: 1.To evaluate the biocompatability of nano-hydroxyapatite crystals and polyamide composite (n-HA/PA66) with the L929 cells.To proceed the morphological observation and take pictures of L929 cells after 1d,2d,4d,and 7d of co-cultured with extract of n-HA/PA66 ,and direct contact with n-HA/PA66.To determine light absorbtion value of every hole under 500 nm with enzyme linked immunity instrument after 1 d,2 d,4 d,and 7 d of contact of n-HA/PA66 extract with L929 cells,and direct contact with n-HA/PA66.In the meanwhile calculate the relative multiplication rate of cells,and evaluate them by six degree tests for cytotoxicity. To investigate the acute and chronic toxic reaction on the whole body induced by the new nano-hydroapatite crystals and polyamide composite(n-HA/PA66)after implanting in vivo and its effects on partial constitution of animal organs after implanting in vivo,and evaluate the potential and degree of subcuticular stimulation reaction.

本实验主要由以下三部分组成：一、n-HA/PA66 复合材料在动物体内、体外的生物相容性及生物安全性评价二、n-HA/PA66 复合材料植入动物体内的生物活性及近期对机体钙、磷代谢影响的实验研究三、网孔 n-HA/PA66 复合材料作为支架修复兔桡骨节段缺损的动物实验研究主要研究目标及方法如下：参照 GB/T16886.5-1997-ISO 10993-5：1992《医疗器械生物学评价细胞毒性试验体外法》之评价标准和要求，采用规定的 L929 细胞(小鼠结缔组织成纤维细胞)，分别经直接接触和材料浸提液与细胞共培养等方式对 n-HA/PA66 复合材料进行细胞毒性测试，采用细胞形态观察法观察两种细胞各组在 24h、48h、72h、5 天后各时相点的细胞形态学变化，并在显微镜下照相，从而对细胞与材料的生物相容性进行定性评价；同时采用细胞生长抑制法，以酶标仪定量测定评价各组 1，2，4，7 天 L929 细胞的相对增殖率，以定量测定并判别材料对细胞的毒性程度。

Soils of plateau land and gully bottom were characterized by the lower pH and catalase activity but higher cation exchange capacity.

不同地形条件下土壤pH值的变化由地形条件引起的土壤过程及硝态氮在土壤中的累积引起；阳离子交换量的变化由成土过程、pH值和有机质的差异引起；土壤有机质及氮、磷养分的差异由与地形条件对应的土地利用方式引起；土壤酶活性的差异则是有机质的差异引起的。

Comparison of different types of stem cutting in rooting induced and analysis the cDNA clones which may be associated with the occurrence of adventitious roots of P.euphratica,the model of ARF were initial establishment of exogenous IBA controlling endogenous IAA occurred threshold and equilibrium,that is,exogenous IBA receiving by receptor of stem cutting,and transfer into the cells,then turn into active IAA by the effects of the POD enzyme.

通过对胡杨3个不定根发生调控培养体系的比较和与胡杨不定根发生可能相关的差异基因的克隆分析，初步建立IBA调控胡杨不定根发生的内源IAA阈值和激素间平衡的模型，即外源IBA通过插穗受体接收，转入细胞内并在POD酶作用下转化成具有活性的IAA，当IAA的水甲在促根的阈值内并保持相对平稳时，则有利于生根；当IAA的浓度增高超过阈值且不稳定时，则抑制不定根的发生。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。