成酶的

Methods The experimental TBI model was established by bumpiness of free falling body according to Feeney′s.The rats′cerebral edema were imaged with magnetic resonance image,The changes of brain water content and permeability of blood-brain barrier were measured by the methods of wet and dry weight and Evans blue fluorometry.The expression of AQP-4 mRNA was examined by reverse transcriptase polymerase chain reaction.

按照改进的Feeney自由落体撞击法建立大鼠创伤性脑损伤模型，用磁共振成像对大鼠脑水肿进行检测，干/湿比重法和伊文思蓝测定法观察大鼠TBI后不同时相脑组织含水量和BBB通透性的变化，并采用逆转录聚合酶链反应法检测AQP-4 mRNA的表达，蛋白质免疫印迹法检测AQP-4蛋白的表达。

On the basis of MEBT, a new technique hypothesis of injecting MEBO under the eschar was proposed to have 6 biochemical reactions available, such as hydrolysis, enzymolysis, saponification, rancidify, lipofication, esterification of MEBO with necrotic tissue of the eschar, then to realize liquefaction and quick defluxion of necrotic tissues, This finally to play a role of regenerative action of MEBO in situ to turn potential regenerative cells into skin stem cells that would further proliferate and differentiate to form skin tissues, reaching the goal of physiologically repairing wounds.

最后提出了在烧伤湿性医疗技术正规治疗的基础上，同时进行痂下注射MEBO的新技术设想，其目的是使痂下组织尽早接受MEBO的治疗，痂下发挥MEBO的水解、酶解、皂化、酸败、脂化、酯化六大生物化学反应，促使坏死组织痂下痂外一起液化，尽快脱落；而且在痂下发挥MEBO原位再生作用，使潜能细胞转变成皮肤干细胞，干细胞进一步增殖分化形成皮肤组织，达到创面生理性修复之目的。

METHODS: Human IPE cells were isolated by enzyme-assisted microdissection method and cultured in plates.

采用酶辅助的显微分离法对成人眼进行IPE的原代和传代培养，观察其形态；以不同浓度的层黏连蛋白（0，1，5，10mg/L）处理第3代IPE细胞，MTT法作出生长曲线，免疫荧光法检测细胞角蛋白1（CK1）表达的变化。

Simultaneously we prepared benzimidazolines with two methods by the reducton of benzimidazolium salts with NaBH_4 and Grignard addition with benzimidazolium salts. Thus we provided a new biomimic synthetic method for 9 kind of tetrahydro-β-carboline starting from carboxylic aicd and halid. A convenient synthetic method for preparing tetrahydro-β-carboline was provided.

本文以1，3-二甲基苯并咪唑碘盐和2-取代的1，3-二甲基苯并咪唑碘盐为原料，采用了NaBH_4还原和Grignard试剂加成两种方法，制备了9种1，3-二甲基苯并咪唑烷和2-取代的1，3-二甲基苯并咪唑烷作为四氢叶酸辅酶一碳单元转移新模型，在酸性条件下，以色胺作为接受一碳转移的亲核试剂，完成9种四氢-β-咔啉类化合物的新合成方法研究。

After irradiation, the survival fraction of the yeasts decreased with increased dose. 2.Colorless TTC can be deoxidized by dehydrogenase into red. As a result, the mutant yeasts overproducing ethanol could be screened out by the color of the colony.

红四氮唑TTC是一种无色显色指示剂，活菌中所含的脱氢酶可将它还原成红色，因此可以根据菌落呈色的深浅判断酵母菌产酒精能力的高低，从而挑选出产酒能力较高的菌株。

CDKI means a family consist of diverse protein, the combination of CDKI and CDK form compound protein, it retrains the catalyze activity of enzyme.

而细胞周期素依赖性蛋白激酶抑制剂是指一个由不同的蛋白质组成的家族，与CDK分子结合成蛋白复合物以后，可以使CDK的酶学催化活性受到抑制。

In this study, the variable region genes of three stem McAb 1A〓-D〓, 2F〓-B〓 and 2E〓-E〓 against metal-bound tetrapeptide were joined into a single chain antibody with a linker respectively. And insert 39bp fragment of N-terminal CGRP to C-terminal of heavy chain and constitute V〓linker-V〓-CGRP single chain genes.

为获得有催化活性的抗体酶，本文将〓和〓〓三株金属螯合四肽单抗的轻、重链可变区基因，通过linker分别连接成单链基因，并在其后插入编码CGRP的N端十三肽的39bp基因片段，组成〓-linke-〓-CGRP，将该基因克隆入分泌表达载体pTC01中，获得表达产物。

To investigate the effects of lipoxygenase (LOX-1) on the cormels formation and enlargement in Gladiolus hybridus, with 'Rose Supreme' and 'Advanced Red' as materials, LOX-1 activity, endogenous methyl jasmonate, sucrose, starch and cellulose contents were examined during the cormels growth.

为探讨脂氧合酶(LOX-1)对唐菖蒲籽球形成和膨大的影响，以'Rose Supreme'和'Advanced Red'两个品种为试材，研究了籽球的生长发育与LOX-1活性，内源茉梨酸甲酯、蔗糖、淀粉和纤维素含量变化之间的关系。

Assay of FAH enzyme activity in skin fibroblasts is possible but not readily available.

皮肤成纤维细胞的FAH酶活检测是可行的但不是现成的。

Results 5'-nucleotidase significantly inhibited platelet aggregations induced by ADP, AA and PAF in a dose-dependent manner.

结果 白唇竹叶青蛇毒5'-核苷酸酶能抑制ADP、AA、PAF诱导的血小板聚集，且其抑制率与剂量增加成正比；抑制血小板聚集作用与ADP的水解和腺苷的积累有关。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。