成酶的

MAIN OUTCOME MEASURES: Preparation of fibroblast feeder layer; growth of embryonic stem cells; results of alkaline phosphatase staining; cell differentiation tendency.

主要观察指标：成纤维细胞饲养层的制备情况，胚胎干细胞的生长状态、碱性磷酸酶染色结果及分化趋势。

Methods The high frequency mutation region G380R of fibroblast growth factor receptor 3(FGFR3) gene was amplified by nested PCR with single lymphocyte and single blastomere.

采用巢式 PCR扩增单淋巴细胞及单卵裂球的成纤维细胞生长因子受体 3基因的高发突变位点 G380 R区域，用限制酶 Bfm 消化 PCR产物，10 %聚丙烯酰胺凝胶电泳检测。

The advantage of supercritical carbon dioxide used as an environmental medium in polymerization and reactions in supercritical carbon dioxide are described. It includes homogeneous free radical polymerizations, precipitation free radical polymerizations, dispersion free radical polymerizations, emulsion and inverse emulsion free radical polymerizations, cationic polymerizations, ring-opening polymerization, melt-phase condensation polymerizations, sol-gel polymerizations, polymer blend synthesis, catalytic chain transfer polymerizations, oxidative coupling polymerization, nitroxide-mediated radical dispersion, atom-transfer radical polymerization, reversible addition-fragmentation chain transfer polymerization, electrochemical polymerization, simultaneous one-pot combination of enzymatic and chemical polymerization, copolymerization of carbon dioxide used as monomer.

评述了ScCO2作为聚合反应介质的优点，以及在ScCO2中可进行的聚合反应类型，包括均相聚合、沉淀聚合、分散聚合、乳液及反相乳液聚合、阳离子聚合、开环聚合、熔融态缩聚、溶液-凝胶聚合、聚合物混合合成、链催化转移聚合、氧化-偶合反应聚合、氮氧自由基可控活性聚合、原子转移自由基聚合、可逆加成-断裂链转移、电化学聚合、原子转移聚合与酶催化开环聚合两种活性聚合在ScCO2中同时聚合、二氧化碳作为原料共聚合等。

The decoction of "descend engyme" is constructed by the combination of many years of experience of the hepatitis-treating and the choicely selection of the ancient prescriptions.

降酶汤组方原则系依据中医辨证施治理论，并在结合导师长期治疗慢性肝病的临床经验基础之上研究配伍而成，以清热利湿为法组成的中药复方。

Cofactors may be inorganic ions or complex organic molecules called coenzymes.

辅助因子可以是无机离子或复杂的有机分子成为辅酶。

For the purpose of constructing mammary gland specific expressional vector, we use the updream regulation sequence of bovine BLG to regulate lacS gene to express in mammary gland. Positive clone cells are selected and purified by the expression Neo gene and report gene EGFP. So the cells can be used as sources to create transgenic animals by nuclear transfer techniques.

本研究以牛β-乳球蛋白基因上游调控成分作为启动子，嗜热菌糖苷酶基因lacS为目的基因，构建了lacS基因乳腺特异表达载体，并通过脂质体介导法转染体外培养的牛胎儿成纤维细胞，借助新霉素抗性基因和增强型绿色荧光蛋白报告基因来筛选、纯化阳性克隆细胞，为利用核移植技术制备转基因动物提供细胞来源。

The effect of muscarinic receptors agonist-carbachol on conjunctival fibroblasts'proliferation was studied by Cell Proliferation ELISA.

Brdu酶联免疫吸附试验检测毒蕈碱受体激动剂carbachol对结膜成纤维细胞增殖的影响。

Methods The retinae of 6 postnatal 2-3 days Sprague-Dawley rats were dissociated into cell suspension with trypsin digestion. The cell suspension was implanted in 96-well culture plates covered with murine-tail collagen in each well(1×105 cells/well). The concentrations of PGP solution were 1g.L-1-10-5g.L-1. The period of culture was 1,3 and 5 days respectively, Then, the OD of living cells in each well was tested by MTT colorimetric microassay.

6只生后2-3d Sprague-Dawley大鼠，视网膜采用胰酶消化法制成细胞悬液后接种于经鼠尾胶原处理的96孔培养板(每孔1×105个细胞)，同时加入1g.L-1-10-5g.L-1PGP及DMEM，分别培养1、3、5d，用MTT法测量OD值。

An enzyme produced in the stomach; when activated by acid it splits proteins into peptones.

胃中产生的一种酶，被酸激活后能将蛋白质分解成蛋白胨。

Methods Thoracic aorta of Wistar rat is obtained, by using a specially made crochet hook, the aorta is over turned, then ligated the two ends, the vascular endothelial cells are wiped off with 0.1% collagen Ⅰ enzyme for 15 minutes.

将大鼠主动脉翻转，两端结扎，用0.1%胶原酶消化血管内皮细胞，剩余血管剪成小块均匀种植于培养瓶底，加入DMEM培养液培养，观察血管平滑肌细胞的生长。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。