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Result The COⅠ probe hybridized well with DNA from Paragonimus westermani, but not with DNA from other species.

将酶切后的COⅠ基因和ITS2基因回收,地高辛标记成探针,尼龙膜上行斑点杂交观察。

The fusion gene was inserted downstream to the MOX promoter of Hansenula polymorpha shuttle plasmid pDGXHP2.0, and the constructed multicopy secretory recombinant plasmid was transformed to Hansenula polymorpha ATCC34438 Ura3(superscript - by electroporation.

将融合基因插入汉逊酵母穿梭质粒pDGXHP2.0的甲醇氧化酶基因启动子下游,构建成多拷贝分泌型重组表达载体。

Chitosan and film-forming properties of polyvinyl pyrrolidone dissolved outstanding performance, made of composite membrane, through the composite membrane-embedded and glyoxal cross-linking role of the glassy carbon electrode have a common fixed-horseradish dismutase enzymes and hemoglobin, Fe 63-/4- body as an electronic transmission.

利用壳聚糖成膜性能及聚乙烯吡咯烷酮优异的溶解性能,制成复合膜,通过复合膜包埋和乙二醛交联作用在玻碳电极上共同固定辣根过氧化物酶和血红蛋白,Fe63-/4-作为电子传递体。

The cells were shammed or irradiated with different dosages of UVB radiation and treated with the above TCM monomers. The damage of fibroblasts was observed with inverted phase contrast microscope.Cellular proliferation activity was detected by MTT method.The secretory volume of IL-6 and TNF-α was measured by ELISE. Results The damage degree of the cells irradiated was dependent with the irradiated doses and the proliferation activity reduced by 28%-44%.

采用30mJ/cm^2、60mJ/cm^2、90mJ/cm^2剂量UVB照射体外培养的原代人皮肤成纤维细胞,以中药单体EGCG和黄芩苷进行干预处理,倒置相差显微镜观察细胞受损程度,以甲唑盐比色试验检测细胞增殖活性,酶联免疫吸附试验检测上清液TNF-α、IL-6分泌量。

ICR fetus mice were sterilely obtained to culture mouse embryonic fibroblasts by tissue pancreatin digestion method.

无菌条件下取出ICR胎鼠,组织块胰酶消化法分离培养小鼠胚胎成纤维细胞,接种在0.1%明胶包被的6孔板中即为饲养层。

Any of several esters, other than the ethyl ester, of carbamic acid .Any of a group of enzymes that catalyze transamination.

苯丙酮尿一种先天性代谢异常,其机体缺乏将苯丙氨酸代谢成酪氨酸所必需的酶。

To utilize trehalose,it must be hydrolyzed by trehalase for glycolysis.

海藻糖必须被海藻糖分解酶分解成葡萄糖才能用于糖酵解,以提供昆虫能量的需求。

The optimal conditions for protoplast isolation and regeneration of 4 auxotrophic mutant strains of Trichoderma were studied.

研究了4种营养缺陷型产纤维素酶菌的原生质体形成和再生条件。

In all cases, the enzymes can be dissociated into two distinct protein components, termed A and B, respectively

在所有情况下,这个酶均可分解成两个不同的蛋白质组分,分别称作A和B。

Methods Human and pig dermal fibroblasts were isolated from the skin by enzymation and cultured in vitro. The second passage of human and pig dermal fibroblasts were collected and seeded at a density of 1.0×10^6 cells/mL onto cylinder-shaped polyglycolic acid scaffolds.

酶消化法获取人与猪皮肤成纤维细胞,培养扩增,取第2代细胞,以1×10^6/mL的细胞密度接种至聚羟基乙酸圆柱形支架上体外培养。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力