成酶的

METHODS: The rib trabeculae were resected and broken, trypsinizated and washed completely by PBS. Bone surface and non-adhesive floating cells in cleaning fluid were observed with inverted microscope. Rib trabeculae was washed by DMEM culture medium once, and cultured in culture bottle. The culture liquid was replaced by new one once a week. The osteoblast was moved from the sclerite a week later. The cells were fused monolayer and could be subcultured 4 to 6 weeks later.

切下人肋骨骨小梁剪碎，胰酶消化后以PBS彻底冲洗直至于倒置显微镜下观察骨片表面及清洗液中无粘着及漂浮的细胞，以DMEM完全培养液清洗骨小梁1次，移入培养瓶内培养，每周更换培养液1次，1周后可见成骨细胞自骨片移出，4~6周细胞融合成单层可作传代培养。

To investigate the effects of basic fibroblast growth factor on necrosis rate, succinate dehydrogenase level and oxygen consumption of the skin flap, 18 Wistar rats were divided into 2 groups.

为了观察碱性成纤维细胞生长因子对大鼠缺血皮瓣坏死率、琥珀酸脱氢酶含量和氧耗量的影响，选用Wistar大鼠18只，随机分为对照组和实验组。

Our factory was founded in 1993, mainly the production of industrial trypsin, in order to soften the leather.

我厂成立于1993年，主要生产工业胰酶，为皮革的软化。

In this fashion the four entering carbons of two acetyl-CoA molecules are conserved to form one 4-carbon dicarboxylic acid.

用这种方式，由两个乙酰辅酶A引进的四个碳，转变成一个四碳二羧酸。

But after much tinkering with different combinations of detergent, ions, and chemical additives, Strynadka's team was able to crystallize the enzyme so that it would diffract x-rays into a pattern that would ultimately reveal its natural structure.

但是在多次与不同结合物去污剂、离子和化学添加剂等组合后， Strynadka 小组终于能够使该酶结晶，以至能经过 x 光衍射成图像，最终显示它的本来结构。

Methods the spf mice were treated with med or optimized med in dose of 29.3 g/kg and 18.6 g/kg per day respectively by intragastric administration for five days continuously. and then, the various dysmnesia mouse models were prepared respectively with scopolamine (3 mg/kg), cycloheximide (120 mg/kg) by intraperitoneal injection and 40% alcohol (0.1 ml/10 g body weight) by intrag astric administration on the sixth day.

将强记汤及天然脑活素分别以 29.3 g/kg和 18.6 g/kg 的量每日经口灌胃给予 spf 级小鼠，连续 5 d，第 6 天给药 1 h 后制备成记忆障碍模型，第 7 天给药 0.5~1 h 后分别用跳台法和避暗法测试记忆成绩；另用不同剂量（50 g/ml 和 150 g/ml）天然脑活素水煎液处理大鼠大脑 b104 cns 神经细胞，以荧光素酶方法检测 creb 表达水平。

Methods The SPF mice were treated with MED or optimized MED in dose of 29.3 g/kg and 18.6 g/kg per day respectively by intragastric administration for five days continuously. And then, the various dysmnesia mouse models were prepared respectively with Scopolamine (3 mg/kg), Cycloheximide (120 mg/kg) by intraperitoneal injection and 40% alcohol (0.1 mL/10 g body weight) by intrag astric administration on the sixth day. Next day, memory result was estimated by using Step-down Test and Dark-avoiding Test after the mice were administered for half or an hour.

将强记汤及天然脑活素分别以 29.3 g/kg和 18.6 g/kg 的量每日经口灌胃给予 SPF 级小鼠，连续 5 d，第 6 天给药 1 h 后制备成记忆障碍模型，第 7 天给药 0.5~1 h 后分别用跳台法和避暗法测试记忆成绩；另用不同剂量（50 g/mL 和 150 g/mL）天然脑活素水煎液处理大鼠大脑 B104 CNS 神经细胞，以荧光素酶方法检测 CREB 表达水平。

Methods Enzyme-linked immunsorbent assaywas adopted to examine the serum basic fibroblast growth facterconcentration of 25 patients with proliferate hemangioma,14 with involuted hemangioma,12 with treated hemangioma by hormone,9 with vascular malformation and 12 of the control group.

方法用免疫酶标技术检测25例增生期血管瘤、14例消退期血管瘤、12例应用激素治疗后血管瘤、9例血管畸形及12例对照组患儿外周血清中碱性成纤维细胞生长因子的浓度。结果25例增生期血管瘤患儿外周血清中bFGF浓度明显高于应用激素治疗后血管瘤、消退期血管瘤、血管畸形及对照组；消退期血管瘤和应用激素治疗后血管瘤患儿血清中bFGF浓度略高于血管畸形及对照组，但差异无显著性。

Our study demonstrate that TNFα and IL-1 have the same function on the odontoclast in the root resorption.

IL-1通过刺激成纤维细胞分泌胶原酶、前列腺素，间接地参与骨、根的吸收。

After osteoplastic induction, peripheral blood MSCs had strongly positive reactions for alkaline phosphatase staining, total collagen staining, alizarin red staining.

外周血间充质干细胞成骨诱导后，碱性磷酸酶染色、总胶原染色及茜素红染色均呈强阳性；成脂诱导后油红染色呈阳性，有一定数量的脂滴形成。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。