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In addition, after stably expression of bFGF, the biological characteristics of BMSCs and bone marrow-derived osteoblasts were analyzed in the fields of morphology, proliferation, cell cycle, alkaline phosphatase, typeⅠ collagen and so on.

从形态、增殖特性和细胞周期、碱性磷酸酶、Ⅰ型胶原等方面分析稳定表达碱性成纤维细胞生长因子的骨髓间充质干细胞和骨髓源性成骨细胞生物学特性。

GFP gene eukaryotic express plasmids were transfected into ES cells. The cells were observed under the inverted fluorescent microscope. The clones which expressed the powerful green fluorescence were chosen to be named ES-GFP cells and cultured continuously. The examination of ES cell totipotent including Alkine phosphatase staining, embryonic body formation in vitro and teratomas formation in nude mice was implemented.

1。采用脂质体方法将GFP质粒转染ES-D3细胞株,经筛选后倒置荧光显微镜观察细胞形态及荧光状态,挑取荧光最强的克隆,命名为ES-GFP细胞,进一步扩增培养,观察细胞生长及荧光表达情况,行细胞分化全能性鉴定包括碱性磷酸酶(alkine Phosphtase,AKP)染色、体外胚胎体(embryonic body,EB)形成实验及裸鼠体内成瘤实验。2。

"When urea is added to soils it is converted rapidly to ammonium carbonate by hydrolysis, a reaction which is activated by enzyme urease."

尿素施人土壤后很快水解成碳酸铵。这是由尿素酶催化的一种化学反应。

The effects of transglutaminase treatment on the properties of edible films cast from soybean protein isolates (SPI1,SPI2),sodium caseinate (NaCN1,NaCN2) and gelatin (G1,G2) were investigated.

研究了谷氨酰胺转移酶对大豆分离蛋白(SPI1和SPI2)、酪蛋白酸钠(NaCN1 和NaCN2)及明胶(G1和G2)3类蛋白质成膜特性的影响。

The possibility of crosslink between sodium caseinate and whey isolateand the impacts on functionality after crosslinking by transglutaminase and the application of transglutaminase on yoghurt were studied.

首先,利用SDS-PAGE电泳结合凝胶成像分析技术,比较了在非变性、加入还原剂(20mmoL/LDTT)变性和在80℃预热15min后并再加入一定量还原剂(20mmoL/LDTT)变性三种条件下转谷氨酰胺酶对2%酪蛋白和乳清蛋白之间的交联情况。

There were three kinds of cellulase which were used to modify the cotton pulp .

本实验初步探讨了3种纤维素酶对棉浆改性后打浆性能及成纸强度性能的影响。

During liquefaction of the ejaculate, the semen coagulum proteins semenogelin I (SEMG1) and semenogelin II (SEMG2) are degraded to low molecular mass fragments by kallikrein-related peptidase 3 (KLK3), also known as prostate-specific antigen.

射精后精液液化的过程中,精液凝结蛋白semenogelin I(SEMG1)和semenogelin II(SEMG2)被血管舒缓素相关肽酶3(kallikrein-related peptidase 3,KLK3)降解成小分子量片断。KLK3也被称作前列腺特异抗原。

A cofactor that is covalently attached to the enzyme is called a prosthetic group.

与酶共价结合的辅助因子成为辅基。

Professor Daniell's breakthrough "uses plant-derived enzyme cocktails to break down orange peels and other waste materials into sugar, which is then fermented into ethanol."

Daniell教授的一个突破就是他利用植物性酶鸡尾酒把橘子皮或其它废物分解成糖,然后再发酵成为乙醇。

Desmin, the marker of myoblasts, was adopted to identify the expression of specific marker protein of sarcoblast desmin with immunohistochemistry. Desmin negative cell clones were removed, and desmin cell clones were cultured continuously with the culture fluid replaced once every other day.

采用成肌细胞特异性标志抗原desmin免疫化学染色,鉴定成肌细胞标志蛋白——结蛋白的表达,弃去desmin阴性的细胞克隆,继续培养desmin阳性的细胞克隆,隔天换液1次,7 d进行酶消化传代,获得大量扩增的细胞,并可冻存复苏,用于实验。

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