成酶的

There was a negative correlation between isoluble amylose content and the activity of active enzyme but a positive correlation between the insoluble amylose content and the ratio of amyl...

不溶性直链淀粉的含量与普鲁兰酶活力成负相关；不溶性直链淀粉的含量与直链淀粉和支链淀粉的比值成正相关，其中四个样品为显著水平，一个样品接近显著水平。

Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ～, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞，经传代纯化后，脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞，500μg/mLG418筛选2周，G418减半继续培养2～3代，然后对转染筛选后的细胞进行PCR检测及核型分析。

DESCRIPTION】the ingredients of the product comes from abyssopelagic fishes of special pure and clean sea areas, adopting Limited Enzymic Degraded Technology, a self-owned intellectual property, that is using compound bioenzyme preparations at a low temperature to extract active ingredients through a special low-temperature extraction process and specific sheared protein to ensure the bioactivity of Collagen.

本品原料来自纯净、无污染的特定海域中的深海鱼类，采用具有自主知识产权的限制性酶切制备多肽技术（Limited Enzymic Degraded Technology），在低温下使用复合生物酶制剂，通过特殊低温萃取工艺提取有效成分，通过特异性的剪切蛋白质，保证了胶原蛋白原有的生物活性，并将原分子量为30万左右的胶原蛋白分子降解成3500左右的胶原蛋白多肽（约含30个左右的氨基酸残基），其分子长度降至30—50nm，集中度达到99.5%以上，呈白色或淡黄色，无异味，无杂物，不添加任何激素和化学物质，没有任何毒副作用。

Our previous studies have shown that ubiquitous overexpression of HmgD caused hemocytes proliferation and the formation of melanotic tumors in the Drosophila larvae. To clarify the mechanism of melanotic tumors appearance,we also generated RT-PCR analysis to test the transcriptional changes of PPAE and Spn27A,which encode the key factors in the phenoloxidasecascade pathway from larvae samples of mid-third instar,late-third instar and prior to pupation relative to wild type flies.Our result showed that in the HmgD overexpression mid-third instar larvae,the expression level.of Spn27A was lower than wild type,otherwise the expression level of PPAE was higher than wild type.It suggests that overexpression of HmgD affects the PO cascade pathway.

本研究组前期工作发现，过量表达HmgD引起果蝇幼虫体内血细胞增生，并出现黑色素瘤，为探讨HmgD过量表达导致了血细胞增生即形成黑色素瘤的分子机制，本研究分别提取三龄中期、后期和相对于野生型化蛹前时刻的幼虫的RNA，首先检测了编码酚氧化酶级联反应途径中的两个关键基因PPAE，Spn27A在突变体中的转录发生变化，结果发现，Spn27A在三龄中期的表达量低于野生型，而PPAE的表达高于野生型，表明过量表达HmgD影响了酚氧化酶级联反应途径，低水平的Spn27A不足以抑制PPAE的活性，活化的PPAE催化了PPO的活性，使其转变成有活性的PO，从而促进黑色素的生成，使果蝇体内产生黑色素瘤。

MAIN OUTCOME MEASURES: The morphology of BMSCs was observed by the inverted phase contrast microscope. Expression of BMSC surface antigens was detected by flow cytometer. Osteogenous potential was assessed by alkaline phosphatase staining. Adipogenic potential was evaluated by Oil red O staining. Chondrogenic potential was assessed by toluidine blue staining.

主要观察指标：倒置相差显微镜下观察骨髓间充质干细胞的生长状态，流式细胞仪检测细胞表面标志物的表达，采用碱性磷酸酶染色鉴定成骨能力，以油红O染色鉴定成脂能力，以甲苯胺蓝染色鉴定成软骨能力。

Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.

应用组织块培养法进行人肝癌伴生成纤维细胞的原代培养，细胞铺满培养瓶底后首次传代，通过胰酶消化法和反复贴壁法进行成纤维细胞的纯化；通过倒置显微镜观察细胞形态、免疫组织化学方法检测细胞膜波形蛋白、角蛋白表达情况，对细胞进行鉴定。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

This equation was tested by the renaturation processes of denatured egg white lysozyme in guanidine hydrochloride and urea solutions, with the results to show that when guanidine hydrochloride and urea concentrations were separately higher than 1.25 and 3.00 mol/L or separately lower than 1.00 and 3.00 mol/L, the refolding intermediates of egg white lysozymes were more easily aggregated to aggregate state or more easily renatured to native state, respectively.

以蛋白溶菌酶在盐酸胍和脲溶液中的复性过程对此关系进行了验证，结果表明，当溶液中盐酸胍和脲浓度分别大于1.25 mol/l和3.0 mol/l或分别小于1.0 mol/l和3.0 mol/l时，它们的折叠中间体分子分别更倾向于集聚成集聚体或更倾向于复性成原始态分子。

The seeds of different varieties of mat rush were treated by 60Co γ-rays irradiation of 500, 1000, 1500 and 2000Gy. The seedlings cultured from treated seeds were used to detect the root activity, seedling growth, chlorophyll content, activities of polyphenol oxidase and catalase, and to investigate the effect of irradiation treatment on the physiological and biochemical properties of mat rush.

分别以500、1000、1500和2000Gy的60Coγ射线处理不同蔺草品种成熟干种子，并将其培养成实生苗，进行根系活力、叶绿素含量及多酚氧化酶和过氧化氢酶活性的检测，观察蔺草苗期的生长状况，从而研究不同辐照剂量对蔺草苗期生长及生理生化特性的影响，确定蔺草的适宜辐照诱变剂量，为下一步开展蔺草品种诱变改良奠定基础。

Short interspersed elements are widespread among eukaryotic genomes and are moderately repetitive DNA sequences. These molecules range from 70-500bp in length. There are usually 103-105 copies of a SINEs family in a eukaryotic genome. The evidences suggested that SINEs have proliferated by retrotransposition.

短散在核重复序列(short interspersed elements SINEs)是广泛分布于真核生物基因组内的中度重复序列，分子长度在70-500bp，在基因组内的拷贝数为103-105；SINEs在基因组内以返转座的方式进行增殖，由RNA聚合酶III转录成RNA，再反转录成cDNA插入到基因组内。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。