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The results prove that CNHK500 has highly proliferation selectivity and potent cytolysis effect on breast cancer in vitro.

在感染CNHK500的端粒酶阴性的正常成纤维细胞株中未检测到E1A基因表达,在端粒酶阳性的感染CNHK500的乳腺癌细胞株中能够检测到E1A基因的表达;CNHK500可以选择性地在缺氧条件下表达E1B。

Methods:(1) ADM was produced from swine skins treated with trypsin followed by Triton X-100.(2) type I collagen of mouse tail was extracted by 0.5mol/L acetoacetic acid.(3) Two kinds of ADM were got by being soaked with 0.02% glutaraldehyde for 5~10 min or being soaked with mouse type I collagen for 24h, then preserved at 4℃.

(1)应用胰蛋白酶消化-去污剂法制备ADM;(2)用0.5mol/L乙酸溶液提取制备I型鼠尾胶原;(3)用0.02%戊二醛溶液浸泡ADM5-10min,制成交联型ADM;另一部分ADM用质量分数0.25%的I型鼠尾胶原浸泡24h,制成胶原包埋型ADM,冰冻保存;(4)用酶消化法培养原代成纤维细胞,取第三代增殖期细胞,将其调整至浓度为2×105/ml的成纤维细胞悬液。

As the results showed that the significant relation between TCBA and calcium or magnesium, mainly between glycocholic, glycochenocholic or taurocholic acids and calcium or magnesium, could be found in all three groups but there was not any obvious relation between TCBA and ferrum or zinc except between glycocholic acid and zinc in gallbladder stone group.

由此可见前列腺素在胆结石形成中占有重要地位。花生四烯酸是合成前列腺素的前体,它在环氧化酶作用下形成前列腺素。本研究证实在成石性胆囊胆汁中花生四烯酸的含量(0.09%)和阳性率(71.74%)明显高于对照组(0.02%,21.42%),P.05。说明成石性胆汁为合成前列腺素提供了前体物质。

Results In define induction envioroment, HPMSCS can diferentiate into lipoblast、after 2 weeks, the induced cells are full of lipid droplet;HPMSCS can differentiate into osteoblasts, after 4 weeks, we can see calcified tubercle in the cells, calcium in the calcified tubercle can be dyed black by Von Kossa and ALP dying is positive reaction above 95%.

结果 HPMSCS在培养基中持续培养后,在特定诱导环境下,成功使其向成脂肪细胞转化,培养2周时,可见融合成团的脂滴充满整个细胞;成功使其向成骨细胞转化,培养4周以上可见明显钙化结节,von Kossa染色可将骨结节中沉积的钙染成黑色,碱性磷酸酶染色呈强阳性反应达到95%以上。

Myoblast differentiation and fusion in multinucleated muscle cells was studied in myoblasts growth in culture. The content ofcalpastatin in muscle wonld be related to cell membrane fusion.

在成肌细胞培养中,成肌细胞融合并分化形成多核肌细胞,其中钙激活酶抑制蛋白的含量与细胞膜的融合相关。

Drugs inhibiting collagen hydroxylation and accumulation are expected to improe this situation, making prolyl 4-hydroxylase (P4H), the key enzyme of intracellular collagen processing, a rational target for pharmacological interention.

抑制胶原羟化和沉积的药物被临床医生所期盼,生成的羟脯氨酸酶{P4H},肝内细胞胶原生成的关键酶,是药物介入的理想目标。

And all the soil samples were collected from the surroundings of cinnamon oil factory, flower fertilizer and so on. On the whole, this research mainly included two aspects:(1) To screen and ferment the strains producing the aimed enzyme and study on the enzymatic synthesis reaction of natural benzaldehyde.

其研究内容主要包括两个方面:(1)产酶菌种的筛选、发酵以及酶催化合成天然苯甲醛反应的研究通过大量实验,采用常规方法培养时,共筛出1 81株对肉桂醛有耐受性的菌株;将常规培养基换成细菌培养基以后,共筛出36株菌。

Using centrifugal casting method, hollow fiber membrane is encapsulated into dialyzers, and prepared by the water, urea, Lysozyme and BSA simulated liquid form, which uses Lysozyme instead of β_2-MG, BSA instead of human serum albumin. In this paper, the simulation solution is used to evaluate dialysis performance of PES hollow fiber membrane dialyzers.

采用离心浇铸法将中空纤维膜封装成透析器,用溶菌酶代替β_2~-微球蛋白,用牛血清白蛋白代替人血清白蛋白,制备由纯水、尿素、溶菌酶和牛血清白蛋白构成的模拟液,用此模拟液来代替患者血液,对本文研制的聚醚砜中空纤维膜透析器的透析性能进行评价。

Enzymatic synthesis of glycerides directly from glycerol and polyunsaturated fatty acids was studied.

研究了在脂肪酶N435的催化作用下,游离多不饱和脂肪酸与甘油酯化合成的工艺,分别考察了甘油与脂肪酸的质量比、反应时间、反应温度、酶用量、初始加水量等单因素对酯化效果的影响。

Smokers with low levels of the enzyme were five to 10 times more likely to develop lung cancer than smokers with the highest levels, researchers at Israel's Weizmann Institute found.

在以色列 Weizmann 研究所的研究人员发现,体内含某种酶水平过低的瘾君子患上肺癌,要比含该种酶高的烟民多上5-10成的几率。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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