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The results have indicated that acid bating fits to bating of sheepskins especially for sheepskin's loose structure because of more alleviate action of acid enzymes, and acid bating has little loss of hide substance.

结果表明,酸性酶软化作用温和、深透,对胶原损害小,特别适合于组织结构疏松的绵羊皮生产。酶制剂用于绵羊服装革鞣前准备其它关键工序,可减少硫化物、表面活性剂等有害物质的用量,提高铬结合,减少作用时间,提高面积得率和成革质量。

The three electrode system which composed of two platinum electrodes and saturated calomel electrode, act as working electrode, counter electrod...

当体液中的尿酸通过酶膜时,与酶进行反应,检测出该处产生的反应电流,然后将其换算成尿酸浓度加以显示。

In this research we obtained the banana ACO gene with about 1.2kb length by cuffing from the cloning vector pUCm-ACO with restriction endonucleases Xba Ⅰ and Sac Ⅰ, and then directionally linked the gene to the plasmid vector pBⅠ-121 in the same endonucleases digestion sites, finally we got the construct of plant expression vector pBI-aACO.

用限制性内切酶Xba Ⅰ和sac Ⅰ从克隆载体pUCm-ACO上切下约1.2kb的香蕉ACO基因,将其定向连接在经相同酶切的质粒载体pBⅠ-121上,构建成植物表达载体pBⅠ-aACO。

The facts that type I compounds could easily undergo deamination reaction to form α-methylene cyclopentanone derivatives and they could react with glutathione to form an adduct provided sufficient evidence for the hypothesis that the compounds acted as masked α-methylenecyclopen- tanones and reacted with biologically important sulfhydry-containing enzymes, and thus produced anticancer activity.

Ⅰ类化合物在稀溶液中极易进行去胺基化反应形成α-亚甲基环戊酮类化合物。并且Ⅰ类化合物于稀溶液中易与谷胱苷肽进行结合,这些事实为Ⅰ类化合物作为隐蔽的α-亚甲基环戊酮与酶进行加成,从而导致酶失活而具有抗癌活性的设想提供了证据。

Enzymatic activity assay of oxalate oxidase in transgenic tobacco plants indicated that the germ in gene from monocotyledon, could be expressed in dicotyledon tobacco plants that resu;ted in the formation of homopentameric protein with enzymatic activity correctly.

酶活性检测表明,源于单子叶植物小麦的germin基因能够在双子叶植物烟草中表达并正常装配成具有酶活性的同源五聚体蛋白。

It catalyzes the oxidation of dopa rapidly to dopaquinone, which in turn polymerize non-enzymetically into insoluble melanin.

它是一种含铜的酶,能够催化单酚羟化成二酚,把二酚氧化成醌;醌在非酶促条件下形成最终的反应产物黑色素。

Cellulases are a family enzymes thatconvert biomass into higher quantities of fermentable sugars, and inturn, into bioethanol.

纤维素酶是一个家庭的酶转化成生物量较高数量的发酵糖类,并反过来,变成乙醇。

METHODS: Fresh bovine tendo calcaneus collagen was dispersed in 0.5 mol/L acetic acid, co-precipitated with chitosan and lyophilized. Dry membranes were cross-linked in 0.05% glutaraldehyde for 24 hours. In vitro its degrading rate was measured by use of collagenase degrading test. The chitosan-collagen membrane was implanted to subcutaneous dorsal sites of SD rats. After implantation, histocompatibility, vascularity and degradation were observed in vivo.

以酸溶法制备胶原溶液,添加一定比例的脱乙酰化几丁质,冻干成膜,0 。0 5 %戊二醛溶液浸泡交联,行体外酶降解实验,检测其耐受酶降解能力;将制备的几丁质-胶原蛋白膜包埋于 36只 SD大鼠皮下,术后 3、5天,1、2、3、4、6、8和 12周取材,检测其组织相容性、血管化能力及材料在体内降解情况。

Then, another systematic study was conducted to enzyme technology in beamhouse operation of sheep garment leather such as soaking, liming, unhairing and deliming.

将酶制剂应用于鞣前准备的浸水、浸灰、脱毛、脱灰等工序,探讨酶技术对成革质量及环境质量的影响,并进行了工艺平衡。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明,所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达;在缺乏AMBN情况下,与对照组相比,实验组牙胚在体外可以继续生长发育至钟状晚期,出现成釉细胞和成牙本质细胞的分化,成釉细胞可以分化成为分泌期型成釉细胞,胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体,缺乏溶酶体,表明对蛋白合成和脚的能力降低;实验组牙胚有牙尖形成和基质分泌,但牙尖形态异常,基质形成减少,牙尖周围基质最厚处为O.6卜m,明显薄于对照组的5.spin,基质中胶原纤维粗细不等,排列稀疏, 3 第四军医大学硕士学位论文未见钙化现象,充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程,影响胶原纤维和牙本质基质的合成,促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

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