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Objective:The aim of this study is to determine the content of vascular endothelial growth factor and basic fibroblast growth factor in the wound fluid during maxillofacial soft tissue blast injury, and to evaluate their effects on angiogenesis of wound healing.Methods: The rabbit model of maxillofacial blast injury was made by KTY_04 blasting cap.

目的:探讨颌面部软组织爆炸伤愈合过程中血管内皮细胞生长因子和碱性成纤维细胞生长因子的分布和在伤口液中含量的动态变化,及其对血管再生的刺激作用。

Objective To determine the content change of vascular endothelial growth factor and basic fibroblast growth factor in the wound fluid and their effect on angiogenesis in the wound healing during healing of maxillofacial soft tissue blast injury. Methods The improved rabbit model of maxillofacial blast injury was made by KTY-04 blasting cap.

摘 要:目的探讨颌面部软组织爆炸伤愈合过程中血管内皮细胞生长因子和碱性成纤维细胞生长因子在伤口液中含量的动态变化及其对创伤愈合中血管再生过程的刺激作用。

Results:Expression of elastase mRNA has been found in the endothelial cells,the medial smooth muscle cells and the adventitial fibroblasts of the abdominal aorta,the lymphocytes,monocytes in blood,the tracheal hyaline cartilaginous cells,the glandular cells of the pancreas,the epithelial cells of the parotid gland and submaxillary gland,the hepatoeytes,the endothelial cells of the liver sinusoid wall,the goblet cells of the mucous membrane of the small intestine,the cardiac myocytes,the renal interstitial fibroblasts,the alveolar epithelial cells,the cerebral glial cells,the fibroblasts of the dermis oorium of the skin,the primary spermaocytes,the secondary spermaocytes and sperm in the seminfferous tubule of the testis,the lymphocytes in the spleen and thymus.

结果正常大鼠腹主动脉的内皮细胞、中膜平滑肌细胞以及血管外膜成纤维细胞,血液细胞中的淋巴细胞、单核细胞,气管透明软骨细胞,胰腺的腺细胞、腮腺、颔下腺上皮细胞,肝细胞、肝窦壁的内皮细胞,小肠黏膜杯状细胞,心肌细胞,肾间质的纤维母细胞,肺泡上皮细胞,大脑胶质细胞,皮肤真皮纤维母细胞,睾丸曲精细管内的初级精母细胞、次级精母细胞以及精子,脾脏以及胸腺的淋巴细胞等,均有弹力蛋白酶mRNA的表达。

Methods Enzyme-linked immunsorbent assaywas adopted to examine the serum basic fibroblast growth facterconcentration of 25 patients with proliferate hemangioma,14 with involuted hemangioma,12 with treated hemangioma by hormone,9 with vascular malformation and 12 of the control group.

方法用免疫酶标技术检测25例增生期血管瘤、14例消退期血管瘤、12例应用激素治疗后血管瘤、9例血管畸形及12例对照组患儿外周血清中碱性成纤维细胞生长因子的浓度。结果25例增生期血管瘤患儿外周血清中bFGF浓度明显高于应用激素治疗后血管瘤、消退期血管瘤、血管畸形及对照组;消退期血管瘤和应用激素治疗后血管瘤患儿血清中bFGF浓度略高于血管畸形及对照组,但差异无显著性。

MethodsThe transplanted tumor model of the mouse S180 sarcoma was established.Fifty mice were randomly divided into five groups,the control group,the CTX group,the Gecko group.They were treated respectively with oral administration of saline,and intraperitoneal injection of CTX 100 mg/kg only one time,oral administration of Gecko in doses of 13.5,9,4.5 g/kg,one time a day.After 14 days,the anti-tumor activity was evaluated by tumor tissue weighing. The impact on immune organ was detected by accounting thymus index, spleen index and the number of peritoneal macrophage which phagocytose chicken red blood cells.The protein express of vascular endothelin growth factor and basic fibroblast growth factor were detected by SABC immunohistochemistry.

方法建立移植瘤小鼠S180肉瘤模型,将50只雌鼠随机分为对照组、环磷酰胺组、壁虎高组、中组、低组共5组,分别给于生理盐水灌胃1次/d,CTX(100 mg/kg)腹腔注射1次,壁虎高、中、低组(13.5,9,4.5 g/kg),每天灌胃1次。14 d后,称取荷瘤小鼠瘤重、胸腺重、脾脏重,计算抑瘤率、胸腺指数、脾脏指数,观察腹腔巨噬细胞吞噬鸡血红细胞实验指标;用SABC免疫组化法检测血管内皮生长因子、碱性成纤维细胞生长因子的蛋白表达,TUNEL方法检测细胞凋亡率。

The COX-2 positive cells were odontoblasts, pulpal fibroblasts vascularendothelial cells and macrophages (positive neutrophils could be found in some cases of the second group and of the third group).(3)We found that COX-2 developed up with HIF-1 a in third group ,but against it infourth group.

COX-2表达阳性的细胞有成牙本质细胞、成纤维细胞、血管内皮细胞和单核巨噬细胞(阳性中性粒细胞可出现在第二组和第三组),HIF-1α与COX-2的表达相关主要在第三组和第四组牙髓标本中体现明显,在第三组二者的变化大致呈平行关系;在第四组基本呈反向变化关系。

The effects of vacuolization on the differentiation of vascular endothelial cells were analyzed from a chemical biochemistry genetics perspective using the blastodisc cells differentiation model induced by FGF with the γ-butyrolactones derivative.

从化学遗传学的角度,利用γ-丁内酯衍生物研究了成纤维细胞生长因子(fibroblast growth factor, FGF)诱导鸡胚盘细胞向血管内皮细胞分化过程中,空泡化对血管内皮细胞分化的影响。

Since an excessive cell growth is considered as the main pathological event, cell proliferation model occupy most of the animal models, and these cells include retinal pigment epithelial cell, fibroblast cell, cartilage cell and vascular endothelial cell.

由于PVR的主要病理变化是细胞的过度增生,因而动物模型多以细胞增生模型为主,细胞种类有视网膜色素上皮细胞、成纤维细胞、软骨细胞、血管内皮细胞等。

Screened with cOnidia of hauIthe mp, the bo of myce1iageednated frOm cOnidia of Prtubo mp was Observd Whe the mixtUr of CS-l l andcs-l2, aS well aS the COmPoUnd cs-7 were Presot. And the mtw of Cs-1l and cs-12shOwed grOWth-inhibitory activity ofendothlial cells and NIH-3T3 fibrobast in the.

稻瘟霉活性筛选结果显示CS-11和CS-12的混合物、CS-7具有使稻瘟霉孢子菌丝体变形的作用;体外药理实验显示,CS-11和CS-12的混合物具有显著的体外抑制血管内皮细胞生长作用及抑制血管成纤维细胞生长作用。

Methods Thoracic aorta of Wistar rat is obtained, by using a specially made crochet hook, the aorta is over turned, then ligated the two ends, the vascular endothelial cells are wiped off with 0.1% collagen Ⅰ enzyme for 15 minutes.

将大鼠主动脉翻转,两端结扎,用0.1%胶原酶消化血管内皮细胞,剩余血管剪成小块均匀种植于培养瓶底,加入DMEM培养液培养,观察血管平滑肌细胞的生长。

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索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

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This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。