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成胶质细胞

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Water, Glycerin, Methylsilanol Mannuronate, Sodium Carboxymethyl Beta-Glucan, Sodium Hyaluronate, Palmitoyl Oligopeptide, Palmitoyl Tetrapeptide-7, Madecassoside, EGF *, FGF *, Telomerase *, Panthenol, Borago Officinalis Seed Oil, Anthemis Nobilis Flower Extract, Tocopherol, Tocopheryl Acetate, Cetyl Palmitate, Citrus Aurantium Dulcis Peel Oil, Citral, Citronellol, Limonene, Linalool, Zea Mays Starch, Butylene Glycol, PEG-40 Hydrogenated Castor Oil, Hydrolyzed Corn Starch, Hydrolyzed Corn Starch Octenylsuccinate, PEG-33, PEG-8 Dimethicone, Hydroxyethylcellulose, PEG-14, Xanthan Gum, Carbomer, Polysorbate 20, Caprylyl Glycol, Phenoxyethanol, Methylparaben.

主要有效成分是:聚羧基甲基β葡萄糖的钠盐,保湿锁水;透明质酸,高效保湿;寡胜肽;四胜肽;羟基积雪草甙,抗炎症,促进胶原蛋白的合成;表皮生长因子EGF,促进细胞新陈代谢;成纤维细胞生长因子FGF,促进胶原蛋白合成;端立酶,一种RNA与蛋白的复合体,可通过合成新的端粒而修补受损的DNA端粒,使细胞持续不停的增生,也就是利用成人干细胞转变为全新的细胞。维他命B5;琉璃苣籽油,含维他命和矿物质;洋柑橘萃取液,舒缓,抗过敏;维生素E;醋酸盐维生素E;橙皮油;水解玉米淀粉。

Methods We isolated and cultured the MSCs, then induced MSCs into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene (collagenase col Ⅰ) col Ⅰ and ALP expression was detected.

使用诱导方法作用于骨髓基质干细胞,使之转化为成骨细胞;用RT-PCR检验成骨细胞;不同浓度的二磷酸盐或骨碎补总黄酮配制成条件培养基,作用于成骨细胞,并分析生长曲线,分析细胞基因碱性磷酸酶和胶原酶Ⅰ(collagenase col Ⅰ)表达情况。

The microstructure of the scaffold was observed under photomicroscope and scanning electron microscopy,and the in vitro degradation time,permeability,tensile-strength,porosity,shrinka...

实验结果显示:支架中的成纤维细胞、脂肪细胞及组织纤维间质完全去除,胶原纤维得到了松散,并维持其原有的天然三维网络多孔结构;该材料透水汽性处于3000g.m-2.d-1左右,适合创面恢复;体外降解时间处于25~50h之间,并可根据需要调整工艺条件控制降解时间;拉伸强度介于10.20~11.50MPa之间,具有良好的拉伸强度;收缩温度介于70~85℃之间。上述结果表明该材料已解决了猪脱细胞真皮基质渗透性差、降解速度过慢的缺点,并且其透气性和拉伸强度高、降解性优良且可控,符合组织工程支架材料的要求。

The alveolitis that produces fibroblast proliferation and collagen deposition is progressive over time.

肺间质发生成纤维细胞的增生,成纤维细胞可以分泌大量的胶原纤维,最终导致了肺间质纤维化。

Methods 12 guinea pigs of 20 months with 3 cm×3 cm hair sheared from their backs were divided into control group and experimental group. Rhodosin and deer serum preparation was besmeared on the skin of guinea pigs in experimental group, and cosmetic matrix was besmeared on the skin of animals in control group for 60 d. The distribution of type Ⅲ collagen was observed with Sirius red staining and HE staining. The fission index of the cells of stratum basale, the thickness proportion of stratum corneum to epidermis, the volume density of the collagen fibers and small blood vessel in dermis, the number of fibroblasts in aging skin were meassured with technique of sterology.

背部被剪去3 cm×3 cm毛的12只20月龄豚鼠被随机均分为给药组和对照组,给药组涂抹红鹿制剂,对照组涂抹化妆品基质,连续涂抹60 d;应用天狼猩红、HE染色和体视学技术分别观察和测定老化皮肤中Ⅲ型胶原的分布、表皮基底层细胞的分裂指数、角质层同表皮厚度的比例、真皮胶原纤维和微血管的体密度和成纤维细胞的数目。

Therefore, our data show that in cases when clear cell chondrosarcoma and chondroblastoma pose a diagnostic challenge, the presence of type II collagen in the extracellular tumour matrix significantly supports the diagnosis of clear cell chondrosarcoma and aids in distinguishing it from chondroblastoma.

我们的数据表明,在诊断透明细胞软骨肉瘤和成软骨细胞瘤受阻时,细胞外肿瘤基质中II型胶原的存在对于诊断透明细胞软骨肉瘤相当有帮助,并有助于和成软骨细胞瘤间的鉴别。

Results After used the SGS , the arrange of collagen tend to unifying , micrangiums are rich and in opening condition, the polymeride of pre-collagen accumulate in cytochylema in fibroblast, the total content of collagen is decreased, but the content of hPcⅢ is kept in a high level, the content of HA is increased gradually,which closes to the normal skin level.

结果 应用硅凝胶膜后,胶原排列趋于一致,微血管丰富而呈开放状态,成纤维细胞的前胶原聚合体蓄积在细胞浆内;胶原总的含量减少,但Ⅲ型前胶原含量维持在一个较高水平;透明质酸含量逐渐升高,接近正常皮肤水平。

Result The flexibility of the scaffold material was powerful. The load-elongation curve of the mechanical testing was similar to that of ACL, the maximum load, the ultimate stress and the Young's modulus of the scaffold materials were 52.61 N, 14.96 MPa and 202.08 MPa respectively. Human ACL cells displayed the representative characteristercs of the ligamentous fibroblasts, and synthesized extracellular matrix, such as type Ⅰand Ⅲ collagen protein. The scaffold materials had no cytotoxicity. Human ACL cells adhered, grew and proliferated well both on the surface and in the holes of the scaffold materials.

结果]韧带支架材料的柔韧性强,拉力测试的负荷-拉伸曲线与韧带的拉伸曲线相似,其最大负荷、极限应力和弹性模量分别为52.61N、14.96MPa和202.08MPa;体外分离培养的人前交叉韧带细胞呈典型的成纤维细胞特征,能在体外分泌Ⅰ、Ⅲ型胶原等细胞外基质;支架材料无细胞毒性,人前交叉韧带细胞可在支架材料上黏附、生长并分泌细胞外基质。

Primary cultured chondrocytes are poly-angle, cytoplasm-rich, and their nuclei are either round or oval with clear necleole. Metachromatic and alcian blue positive staining in primary cultured chondrocytes was observed. Intercellular matrix was anti-collagen type Ⅱ staining but not anti-collagen type Ⅰ staining by IHC assay.

原代培养软骨细胞呈多角型,胞质丰富,胞核成圆形或椭圆型,核仁清楚,甲苯胺蓝呈异染性,阿尔新蓝8Gx 染色阳性,细胞外基质Ⅱ型胶原免疫组化染色阳性,Ⅰ型胶原染色阴性。

Immunoelectron microscopy showed that the distinct ultrastructure of fibroblast and the group, spot gold particle specificity distribution shape. The positive markers of CTGF were mainly located in the RER,while there are expression in RER ribosome, Cf, ECM, desmosome connection, euchromatin,et al.

免疫电镜标记显示成纤维细胞细胞超微结构清晰,金颗粒呈团状、点灶状分布,特异性强,CTGF蛋白阳性标记主要位于粗面内质网,粗面内质网核糖体、胶原纤维、细胞外基质、桥粒连接、常染色质等部位也有表达。

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