成胶质细胞

Neuroectoderm; neural crest; neural plate; floor plate; neural tube; neuraxis; neuroblasts; neural stem cells; neuroprogenitor cells; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; cell migration; neurogenesis; development; developmental stages

神经外胚层；神经脊；神经板；底板；神经管；轴索；成神经细胞；神经干细胞；神经母细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；神经节发生；胚胎发生；轴突发生；视网膜发生；神经胶质发生；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管缺陷；无脑；脊柱裂；细胞迁移；神经发生；发展；发育阶段

Neuroectoderm; neural crest; neural plate; floor plate; neural tube; neuraxis; neurulation; neuroblasts; neural stem cells; neuroprogenitor cells; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; neurogenesis; development; developmental stages

神经外胚层；神经脊；神经板；底板；神经管；轴索；神经胚形成；成神经细胞；神经干细胞；神经母细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；gangliogenesis；胚胎发生；axonogenesis；retinogenesis；gliogenesis；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管故障；无脑；脊柱裂；成神经细胞瘤细胞；神经发生；发展；发展进程

Neuroectoderm; neural crest; neural plate; floor plate; neural tube; neuraxis; neurulation; neuroblasts; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; cell migration; neurogenesis; development; developmental stages

神经外胚层；神经脊；神经板；底板；神经管；轴索；神经胚形成；成神经细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；gangliogenesis；胚胎发生；axonogenesis；retinogenesis；gliogenesis；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管故障；无脑；脊柱裂；成神经细胞瘤细胞；细胞迁移；神经发生；发展；发展进程首页上一页下一页末页共有 22 条记录，本页从第 11 到第 20 条。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

Results:Expression of elastase mRNA has been found in the endothelial cells,the medial smooth muscle cells and the adventitial fibroblasts of the abdominal aorta,the lymphocytes,monocytes in blood,the tracheal hyaline cartilaginous cells,the glandular cells of the pancreas,the epithelial cells of the parotid gland and submaxillary gland,the hepatoeytes,the endothelial cells of the liver sinusoid wall,the goblet cells of the mucous membrane of the small intestine,the cardiac myocytes,the renal interstitial fibroblasts,the alveolar epithelial cells,the cerebral glial cells,the fibroblasts of the dermis oorium of the skin,the primary spermaocytes,the secondary spermaocytes and sperm in the seminfferous tubule of the testis,the lymphocytes in the spleen and thymus.

结果正常大鼠腹主动脉的内皮细胞、中膜平滑肌细胞以及血管外膜成纤维细胞，血液细胞中的淋巴细胞、单核细胞，气管透明软骨细胞，胰腺的腺细胞、腮腺、颔下腺上皮细胞，肝细胞、肝窦壁的内皮细胞，小肠黏膜杯状细胞，心肌细胞，肾间质的纤维母细胞，肺泡上皮细胞，大脑胶质细胞，皮肤真皮纤维母细胞，睾丸曲精细管内的初级精母细胞、次级精母细胞以及精子，脾脏以及胸腺的淋巴细胞等，均有弹力蛋白酶mRNA的表达。

Neuroectoderm; neural crest; neural plate; floor plate; neural tube; neuraxis; neurulation; neuroblasts; neural stem cells; neuroprogenitor cells; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; neurogenesis; development; developmental stages

神经外胚层；神经脊；神经板；底板；神经管；轴索；神经胚形成；成神经细胞；神经干细胞；神经母细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；神经节发生；胚胎发生；轴突发生；视网膜发生；神经胶质发生；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管缺陷；无脑；脊柱裂；成神经细胞瘤细胞；神经发生；发展；发育阶段

Neuroectoderm; neural crest; neural plate; floor plate; neural tube; neuraxis; neurulation; neuroblasts; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; cell migration; neurogenesis; development; developmental stages

神经外胚层；神经脊；神经板；底板；神经管；轴索；神经胚形成；成神经细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；gangliogenesis；胚胎发生；axonogenesis；retinogenesis；gliogenesis；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管故障；无脑；脊柱裂；成神经细胞瘤细胞；细胞迁移；神经发生；发展；发展进程下一页末页共有 23 条记录，本页从第 1 到第 10 条。

BACKGROUND: Bone marrow mesenchymal stem cells can differentiate into osteoblasts, chondrocyte, sarcoblasts, adipocyte, neuroepithelial cells, as well as vascular endothelial cells under different conditions.

背景：骨髓间充质干细胞在不同条件下可以分别分化为成骨细胞、软骨细胞、成肌细胞、脂肪细胞、神经胶质细胞、血管内皮细胞等。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果：用诱导分化液作用后，未见小的、伴有突起的放射状形态的神经细胞；抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性； C2C12细胞β Tubulin Ⅲ抗体染色阴性；上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

Results Most OECs were bipolar or tripolar, and the purity was estimated to be over 88.7%. The non-OECs cells consists of astrocytes, neurons, oligodendrocytes and fibroblas.

结果 体外培养的新生小鼠嗅鞘细胞主要为双极或三极细胞，其纯度可以达到88.7%以上，污染细胞中有星形胶质细胞、神经元、少突胶质细胞和成纤维细胞。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。