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During the process of peptization and hydrothermal crystallization, the aggregation behavior and the resulting morphology of nanocrystalline titania (TiO2) were studied by X-ray diffraction, scanning electron microscope, and dynamic light scattering techniques.

通过XRD、SEM和动态光散射粒度测定仪研究了胶溶及水热晶化过程中纳米TiO2晶粒聚集行为及形貌。实验结果表明,在胶溶无定形沉淀过程中所形成的大小为10-15nm的锐钛矿型胶粒或金红石型胶粒易定向聚集成更大的、具有(30-50)nm×(80-100)nm的次级晶粒。含有次级晶粒的溶胶前驱液在水热晶化过程中,次级晶粒发生崩裂并生长成结晶度更高的大小约为10-30nm球形和大小约为20-60nm棒状纳米TiO2

Methods:(1) ADM was produced from swine skins treated with trypsin followed by Triton X-100.(2) type I collagen of mouse tail was extracted by 0.5mol/L acetoacetic acid.(3) Two kinds of ADM were got by being soaked with 0.02% glutaraldehyde for 5~10 min or being soaked with mouse type I collagen for 24h, then preserved at 4℃.

(1)应用胰蛋白酶消化-去污剂法制备ADM;(2)用0.5mol/L乙酸溶液提取制备I型鼠尾胶原;(3)用0.02%戊二醛溶液浸泡ADM5-10min,制成交联型ADM;另一部分ADM用质量分数0.25%的I型鼠尾胶原浸泡24h,制成胶原包埋型ADM,冰冻保存;(4)用酶消化法培养原代成纤维细胞,取第三代增殖期细胞,将其调整至浓度为2×105/ml的成纤维细胞悬液。

Hyacinth bletilla decocted water have inhibition effect on fibroblastic activity from rabbit biliary duct.

白及胶对日本大耳白兔胆管成纤维细胞形态的影响及白及胶对日本大耳白兔胆管成纤维细胞活性的抑制作用,可能是白及胶防治日本大耳白兔腹腔粘连的机制之一。

There was obvious difference on fibroblastic activity between control group and Hyacintn bletilla group at 0.1,1.0,10.0 mg/mL on 72 h(P<0.01,P<0.05).Conclusion Hyacinth bletilla decocted water have inhibition effect on fibroblastic activity from rabbit biliary duct.

白及胶对日本大耳白兔胆管成纤维细胞形态的影响及白及胶对日本大耳白兔胆管成纤维细胞活性的抑制作用,可能是白及胶防治日本大耳白兔腹腔粘连的机制之一。

Materials and Methods: Six patients with left frontal lobe tumors (4 gliomas, 1 germinoma, 1 intra-axial cavernoma) close to Brocca and Wernicke language areas were studied. BOLD and DTI were performed on a 3.0T magnet GE Signa Excite~(? HD_X, BOLD using the word association paradigm developed in Part I and DTT technique developed in PartII. Language activation maps and fiber tracts were superimposed upon high resolution T1 weighted anatomic images of the brain for displaying the relationship between tumor, language functional areas and white matter tracts.

材料和方法:使用GE 3.0 T Singa Excite~ HD_x超导磁共振成像系统,对6例左侧语言相关脑区肿瘤(Ⅲ-Ⅳ级胶质瘤1例,Ⅱ级胶质瘤3例,生殖细胞瘤1例,海绵状血管瘤1例)行BOLD-fMRI及扩散张量成像扫描,BOLD应用语义相关判断作为任务刺激,所有数据进行离线后处理,应用AFNI软件处理BOLD图像,观察语言功能皮层的激活;DTT采用日本东京大学的Volume-one1.56和diffusion TENSOR Visualizer 1.5进行纤维束成像处理。

Results After used the SGS , the arrange of collagen tend to unifying , micrangiums are rich and in opening condition, the polymeride of pre-collagen accumulate in cytochylema in fibroblast, the total content of collagen is decreased, but the content of hPcⅢ is kept in a high level, the content of HA is increased gradually,which closes to the normal skin level.

结果 应用硅凝胶膜后,胶原排列趋于一致,微血管丰富而呈开放状态,成纤维细胞的前胶原聚合体蓄积在细胞浆内;胶原总的含量减少,但Ⅲ型前胶原含量维持在一个较高水平;透明质酸含量逐渐升高,接近正常皮肤水平。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色:翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成;基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质,在翼状胬肉体部深层基质中存在粗糙的纤维组织;正常球结膜组织细胞排列整齐;基质为疏松结缔组织,胶原纤维平行排列,其间可见成纤维细胞,散在少量中性粒细胞、毛细血管;Masson染色:翼状胬肉浅层基质中存在致密的胶原纤维染色,深层基质中的胶原纤维存在变性样改变;VG染色:翼状胬肉组织深层基质中存在大量变性的胶原纤维,其间夹杂黑色的弹性纤维;免疫组化染色法:MMP-3在翼状胬肉上皮细胞中呈强表达,成纤维细胞中呈中等强度表达,血管内皮细胞中未见表达;LN在血管壁中呈强表达,在上皮细胞基底膜中呈中等强度表达,在整个基质中未见明显表达;col Ⅲ在整个翼状胬肉基质中呈强表达。

From the middle to the south in the research area, the compaction, the pressure dissolves the function and the structure disruption intensity enlarges gradually, the silicon nature agglutinates the content to increase gradually, and the corrosion function intensity reduces gradually. The diagenesis stage is carried on according to the division standards of secondary enlargement degree, pellet contact type as well as porosity type, the authigenic mineral characteristic, agglutinates, the diagenesis of Xujiahe formation is thoughted degree to be intense, which already entered the late diagenesis stage A sub-stage.

从研究区中部向南部,压实、压溶作用和构造破裂作用的强度逐渐加大,硅质胶结物含量逐渐增多,溶蚀作用强度逐渐降低;并根据成岩作用类型、自生矿物特征、胶结物的次生加大程度、颗粒接触类型以及孔隙类型等划分标准,对须家河组进行了成岩作用阶段划分,认为研究区须家河组成岩作用程度强烈,已经进入晚成岩作用阶段A亚期。

As pH value varied in the course of diagenesis,reservoirs experienced one to three cementations or dissolution generations.Lots of secondary dissolution pores developed in the late diagenetic stage,because of the acidic mediums in the process of generation and migration of oil and gas. The anhydrites deposited in the dissolved pores resulted in the heterogeneity of secondary pores.

储层在成岩过程中受地层水pH值变化的影响,经历1~3个世代的胶结或溶蚀作用,其中在晚成岩期油气生成和运移过程中形成的酸性成岩环境对早成岩期生成的碳酸盐岩胶结物和储层中的长石矿物溶蚀,因而产生了大量的次生溶孔,但随后在溶孔中沉淀了不等量的硬石膏,导致了次生孔隙的非均质性。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明,所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达;在缺乏AMBN情况下,与对照组相比,实验组牙胚在体外可以继续生长发育至钟状晚期,出现成釉细胞和成牙本质细胞的分化,成釉细胞可以分化成为分泌期型成釉细胞,胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体,缺乏溶酶体,表明对蛋白合成和脚的能力降低;实验组牙胚有牙尖形成和基质分泌,但牙尖形态异常,基质形成减少,牙尖周围基质最厚处为O.6卜m,明显薄于对照组的5.spin,基质中胶原纤维粗细不等,排列稀疏, 3 第四军医大学硕士学位论文未见钙化现象,充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程,影响胶原纤维和牙本质基质的合成,促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

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