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Objective To study the relationship between intra cellular actin and scar contracture.

目的 探讨增生性瘢痕、瘢痕疙瘩成纤维细胞中肌动蛋白与瘢痕挛缩的关系。

BACKGROUND: Muscle-derived stem cells have some problems in purification, amplification and directional differentiation during in vitro culture. Basic fibroblast growth factor as a membrane of bioactive factor has been paid great attention, due to its biological activity.

背景:肌源性干细胞在体外培养过程中存在纯化、扩增、定向分化等难题,碱性成纤维细胞生长因子作为生物活性因子的一员,因其生物活性的多效性而备受关注。

OBJECTIVE: To explore effects of basic fibroblast growth factor on in vitro proliferation of rat muscle-derived stem cells.

目的:探讨碱性成纤维细胞生长因子对大鼠肌源性干细胞体外增殖的影响。

Objective To study the significance of myofibroblast in the process of iatrogenic biliary stricture.

目的探讨肌成纤维细胞在医源性胆管狭窄形成过程中的作用及意义。

Aim: To study the formation and significance of myofibroblast in theca layer of rat ovary.

目的:探讨肌成纤维细胞在卵泡膜中的形成及意义。

Using cytochalasin D to prevent global actin from forming filamentous structures, the production of Heliothis armigera nuclear polyhedrosis virus in Hz-AM1 cells grown in the medium containing 0.5μg/ml CD was completely inhibited while its production was reduced to 10〓 in the presence of 0.1μg/ml CD by plaque assay. However, Western blotting revealed that the actin quantity in infected host cells treated or untreated with CD was almost identical. Additionally, CD had no effect on the DNA synthesis of HaNPV.

Western blot表明细胞松弛素D并不影响受染细胞中肌动蛋白的含量,Dot blot表明病毒DNA的合成也没有受到影响,但电子显微镜超薄切片发现在0.5ug/mL细胞松弛素D中形成的病毒粒子形态与正常的有明显区别,认为细胞松弛素D抑制棉铃虫核型多角体病毒的增殖是因为抑制病毒的DNA和蛋白组装成完整有感染性的病毒粒子,即丝状肌动蛋白对病毒粒子的脱壳和DNA的复制、转录没有影响,但对病毒粒子的组装是必须的。

In general, our primary culture performed better in myogenic differentiation, while the externally obtained cells were superior in the odontogenic/osteogenic and chondrogenic differentiation pathways.

原代培养的 hDPSCs 成肌分化能力强,而冻存的细胞牙向/骨向、软骨向分化能力强。

On the one hand, the inflammatory cells and inflammatory mediators phagocytize necrosis cells and impaired cell debris; on the other hand, they activate muscle satellite cells (locate among the basilar membrane and sarcolemma, and in dormancy state when quite) by secreting growth factor to proliferate to form new myotube cells which develop muscle fiber, repair impaired muscle fiber and accomplish muscle regenerative process.

炎症因子在损伤部位发挥着双重作用,一方面吞噬坏死细胞和损伤的细胞碎片,另一方面通过分泌生长因子激活肌卫星细胞(安静时处于休眠状态的,位于基底膜和肌膜之间),使其增殖形成新的肌管细胞进而发育成肌纤维,修复损伤的肌纤维,完成肌肉的再生过程。

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L al loys in esophagus.The extract fluid from Ni-Ti,317L alloys was made according t o the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily ,then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagu s embedding test of Ni-Ti,317L alloys was made according to ASTM standards of U .S.A.The tissue around the alloys was taken at weeks 2 and 12,and the pathologi c changes were analysed.

为探讨新型支架材料Ni-Ti、317L合金在食管局部的致纤维化作用,按美国ASTM标准制备NiTi、317L合金的金属浸提液;&组织块培养法&原代培养食管壁疤痕的成纤维细胞,传代后以金属浸提液进行培养,分组后分别培养4、24、48、72 h,MTT法检测不同培养时间后Fb增殖功能的变化;按美国ASTM标准进行NiTi、317L合金试件的食管壁内包埋实验,即将金属试件经表面处理后直接置入食管壁粘膜层与肌层之间,术后2、12周取出包埋组织,分析试件周围组织的病理变化,并进行胶原纤维染色,观察纤维形成状况。

Organic nitrates are metabolised to NO in the muscle cell via a sulfhydryl dependent mechanism. they are therfore thought to be the physiological substitute for EDRF.

有机的硝酸盐在肌细胞中可以通过一种硫氢基依赖机制被分解成一氧化氮,因此被认为是EDRF的生理学替代物。

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