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成肌细胞

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In the pathological proceeding of pulmonary fibrosis, myofibroblast plays an important role, such as: the abnormal extracellular matrix deposition causing by the remarkable capability of collagen synthesis, derogation of the lung compliance owning to the contractibility of myofibroblast,secreting many cytokines,which contribute to the damage of the alveolar epithelial cells.

肌成纤维细胞在PF的病理进程中扮演着重要的角色,其胶原合成能力强可造成细胞外基质的异常沉积、具收缩性使肺顺应性下降、分泌多种炎性介质加重肺泡上皮损伤。

Methods: Tissue of rat ovary was obtained from 30 healthy sexually mature non-mated femal SD rats. The rats were divided into two groups (oestrus group and anoestrus group). The expression of α-SMA and vimentin were detected and evaluated by immunohistochemistry and image analysis system in the theca cells of different type follicles.

分别取动情期和非动情期大鼠卵巢,运用免疫组化方法检测肌成纤维细胞的特征性标记和波形蛋白分别在这两组中各级卵泡卵泡膜细胞上的表达情况,并进行图像分析。

In this study, we isolated a clonogenic cells from adult human dental pulp. They produced calcified nodules in differentiation inducive medium. When transplanted into immunocompromised mice, they generated a dentin-like structure and human odontoblast-like cells.

主要结论: 1。从成人健康阻生齿中分离到的部分细胞具有一定的克隆形成能力,在诱导剂的作用下,还可向脂肪细胞、肌细胞及成牙本质细胞方向分化,具有多向分化能力,符合干细胞的特性。

BACKGROUND: Under different induction conditions, bone marrow mesenchymal stem cells can differentiate into the mesodermal tissues such as osteoblasts, chondroblasts, muscle cells, adipocytes and so on.

背景:骨髓间充质干细胞在不同诱导条件下具有向中胚层组织细胞如成骨细胞、成软骨细胞、肌细胞、脂肪细胞等分化的能力。

Myoblastic cells from patients with PM had a weaker ability of developing into the myotubules, thus they were unable to perform effective regeneration, which resulted in a progressive necrosis. The exogenous bFGF could promote the division and proliferation of the muscle cells in vitro . These results shield a light on bFGF's potential role in preventing and treating PM.

PM患者受损腓肠肌在体外有再生能力,但融合成肌管能力极弱,存在再生障碍,不能对变性、坏死的肌纤维形成有效再生,故呈进行性肌纤维耗竭;外源性bFGF可促进PM患者体外培养骨骼肌细胞增殖分裂,并可促进肌管成熟,对进展性肌病应具有防治作用。

Methods 11 cases of normal and denervated human skeletal muscle at different intervals were involved in the experiment.

在人体骨骼肌的胚胎发育过程中,肌细胞生成素伴随成肌过程而呈高水平表达。

Coculturing of the transfected fibroblasts with neonatal rat ventricular myocyte cultures resulted in a significant reduction (68%) in the spontaneous beating frequency of the cultures compared with baseline values and cocultures seeded with naive fibroblasts.

与基线值和原始的成纤维细胞的再复制相比,乳鼠心室肌细胞培养基里转录的成纤维细胞的再繁殖,导致了培养基里自律性频率的显著下降(68%)。

Coculturing of the transfected fibroblasts with neonatal rat ventricular myocyte cultures resulted in a significant reduction (68%) in the spontaneous beating frequency of the cultures compared with baseline values and cocultures seeded with naive fibroblasts.

结果发现,与基线、与幼稚成纤维细胞共培养相比,转染的成纤维细胞和新生大鼠心室肌细胞共培养导致培养物自发搏动频率的下降(68%)。

Make a copyof rat pulmonary fibrosis by infusing bleomycin through trachea , isolate and the pulmonary fibroblast, then interfered by atorvastatin ,divided into 3 groups of vacant ,TGF-β1 induced and atorvastatin interceped of different dosage , observe the structure of the cell by fiberscope ,the multiplication of the cell by MTT counting method, the expression of theα-SMA by immunity tissue chemistry staining method .

气管内注入博莱霉素(bleomycin,BLM)复制大鼠肺纤维化模型,并分离培养肺成纤维细胞,用阿托伐他汀对其进行干预,分为空白组、TGF-β1诱导组和不同剂量阿托伐他汀阻断组,用倒置纤维镜观察细胞的形态结构,MTT计数法观察各组细胞的增殖情况,免疫组化染色法定性观察α-平滑肌肌动蛋白(α-smooth muscleactin ,α-SMA)的表达情况。

In the rat carotid injury model, SM16 dosed once daily orally at 15 or 30 mg/kg SM16 for 14 days caused significant inhibition of neointimal thickening and lumenal narrowing. SM16 also prevented induction of adventitial smooth muscle alpha-actin-positive myofibroblasts and the production of intimal collagen, but did not decrease the percentage of proliferative cells.

在大鼠颈动脉损伤模型中,每日口服15或30mg/kgSM16持续14天能够显著抑制新生内膜增厚及管腔狭窄,预防对外膜α-SMA阳性肌成纤维细胞的诱导和内膜胶原的合成,但并不降低增殖细胞的比例。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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