成纤维细胞的

Result show:the solution,extracted from rat rail tendons,consist mainly of type I collagen;No growth of bacteria in 37℃ thermostatic culture box for 72 hours;Absorbing the height of ultraviolet-spectrophotometry is 230 nm by using flicker photometer examination;Amino acid in type I collagen consist of aminoacetic acid(40 per cent) and proline(14.3 per cent);Under microscope:collagen gel netted constraction consist of original collagen fibril that had been fairly well-distributed and had a quite regular striate lines ;The collagen solution prepared collagen gel matrixes in 37℃ thermostasis for culture cells and also prepared collagen solid film by washing collagen gel with a phosphate solution for plasty surgery materials.

结果：提取液主要为Ⅰ型胶原蛋白；72小时37℃恒温培养箱内培养无细菌生长；紫外分光光度计测定，吸收高峰为230nm；氨基酸成分主要为甘氨酸：占40%，脯氨酸：14.3%。显微镜下见：相互交织的胶原原纤维结构组成的网状结构；纤维均匀，有规则横纹。胶原蛋白液在37℃恒温下，可制成凝胶，用于培养细胞；用磷酸液冲洗胶原蛋白凝胶可成固体片状，制成胶原膜，用作医用材料。

Results Fibronectin existed in dental pulp and predentin, and also in space between the wall of dentinal tubule and process of odontoblast in mature dentin. They were mostly distributed in odontoblast layer and round blood vessels and nerves.

结果 纤维粘连蛋白免疫反应普遍存在于牙髓、前期牙本质和成熟牙本质的牙本质小管与成牙本质细胞突起，以成牙本质细胞层，牙髓血管与神经周围最为丰富。

In the leaves of Liriodendroideae, some of the abaxial epidermal cells are papillose and the vascular tissue of the main vein appears to be separated. However, papillose were not found and there are uniseriate, multicellular or unicellular hairs distributed on the epidermis, and the vascular tissue of the main vein appears to be continuous in the leaves of Magnolioideae. Furthermore, in the Magnolioideae, the structure of the leaves of plants in Manglietia are different from that of Magnolia.

结果表明：鹅掌楸亚科和木兰亚科在叶的结构上的主要区别是鹅掌楸亚科两种植物叶的部分下表皮细胞呈乳突状，且整个细胞外壁只形成一个乳突，而在木兰亚科的植物中有单列多细胞或单细胞的表皮毛，未发现乳突；鹅掌楸亚科植物的叶主脉维管组织环分隔呈束状，且其外包被的纤维也排列成束状，而木兰亚科的80种1亚种植物中，叶主脉维管组织连成轮状，其外面也由一圈连续的纤维环所包围。

Most endothelial cells fell off, elastic fibre autolyze; the number of SMC decreased, some SMC nucleus contracted completely or depressed, typical apoptosis body were seen in later stage, muscle silk autolyzed and disappeared mostly; in outer layer saw much collagenic fiber, arranged unorderly; inflammation cells were seen in the aneurysm wall(hypertrophy cell et al.).

多数瘤壁的内皮细胞溶解脱落，不连续，弹力纤维自溶；动脉瘤血管壁均可见SMC明显减少，较多SMC染色质边聚、固缩，部分SMC核全部固缩或出现不规则凹陷，SMC核胞浆或胞核部分出现脱落或裂解成碎片，晚期可见典型的凋亡小体出现，肌丝大都自溶消失：外膜可见大量的胶原纤维，排列较紊乱；瘤壁还可见炎性细胞浸润。

Results :①The MVs were formed by osteoblast of the secret type、dentinoblast and process;② There was a mature process of the MVs in the extracellular matrix.The appearance of the hydroxyapatite was a major mature feature;③ HAP formed initially in the MVs entered into collage matrix and eventually mineralized;④ The shifting sign of HAP from MVs to the surface of collage fibril was observed.

结果：①MVs是由分泌型成骨细胞、成牙本质细胞及其突起芽生进入胶原基质；②MVs在细胞外胶原基质中有一个逐渐成熟的过程，并以羟基磷灰石（hydroxyapatite，HAP）晶体的出现为其主要特征；③HAP在MVs内形成、增多，最终进入胶原基质并进一步沉积、钙化；④部分胶原原纤维表面沉积的HAP 晶体与MVs分泌的晶体有明显的移形迹象。

In four patients with bilateral total corneal stem-cell deficiencies, 3-by-3-mm specimens of oral mucosal tissue were harvested, and the harvested cells were cultured for two weeks with 3T3 feeder cells that had been treated with mitomycin C. Conjunctival fibrovascular tissue was surgically excised from the ocular surface, and sheets of cultured autologous cells were transplanted directly to denuded corneal surface of one eye of each patient, without using sutures.

在4位双侧干细胞完全缺失的病患，取下3乘3的口腔黏膜组织样本，而且被取下的细胞与使用3T3细胞铺成的细胞层以mitomycin C处理，共同培养2个星期；结膜纤维血管组织利用外科从角膜表面切除，而且培养的自体细胞直接地移植到每位病患一只眼睛裸露的表面，不需要缝合。

Results Microscopically, the tumor cells were epithelioid or short spindled and special nests of tumor cells separated by arboring fibrovascular septa. Such morphological variants of CCSK as myxoid degeneration, microcysts formation and sclerosing pattern of extracellular hyalined collagen simulating osteoid were present in some regions.

结果 镜下见瘤细胞为上皮样或短梭形，被分枝状纤维血管间质分隔成巢团状，部分区域见黏液样变性微囊肿和细胞外胶原玻璃样变类似骨样组织的硬化型等形态变异。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

Results Fibronectin existed in dental pulp and predentin, and also in space between the wall of dentinal tubule and process of odontoblast in mature dentin. They were mostly distributed in odontoblast layer and round blood vessels and nerves. In the crown pulp, integral optical density, length density and volume density were 0.49±0.33,38.44±20.7 and 24.56±10.8 respectively.

结果 纤维粘连蛋白免疫反应普遍存在于牙髓、前期牙本质和成熟牙本质的牙本质小管与成牙本质细胞突起，以成牙本质细胞层，牙髓血管与神经周围最为丰富。

The reticulated vessel which has large cavity has thicker wall due to more elastic fibers and more smooth muscles and its endothelium is cuboidal epithelium which has rotundity karyons. But the reticulated vessel which has small cavity has thinner wall due to less elastic fibers and less smooth muscles and its endothelial cells are flat epithelial cells which have long-shuttle-shaped karyons. In some regions, the ducts are very small in diameter and thin of wall, and they become blood sinus in the end.

管腔大者，管壁厚，弹性纤维、平滑肌纤维多，内膜靠腔面内皮细胞多成立方状，细胞核端位近圆形；管腔小者，管壁薄，弹性纤维、平滑肌纤维少，内皮细胞扁平、排列稀疏，胞核长梭形；一些区域管径极小，管壁极薄，成为开放的血窦，只允许一个血细胞通过。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。