成纤维细胞的

Pro-collagen type I secretion by human fibroblasts stabilized during long-term culture, providing a continuous and functional human dermal matrix.

人的成纤维细胞通过长期的培养稳定的分泌前胶原蛋白 I ，在长期的培养过程中还提供了持续的和有功能性的人的真皮模型。

The pcDNA〓-apoE〓 plasmid was transfected to SK-N-SH Neuroblastoma cell by liposome. It could be screened by G〓. The immunohistochemical assay was shown that neuroblastoma cell transfected by pcDNA〓-apoE〓 expressed the recombinant apoE protein. The intracellular expressed recombinant protein could inhibit the death of neuroblastoma cell induced by beta amyloidal peptides 25-35 fragment.

用pcDNA〓—apoE〓真核表达载体与脂质体共转染神经成纤维胶质瘤细胞，用G〓选择压力筛选，细胞免疫荧光检测表明，pcDNA〓—apoE〓转染的神经成纤维胶质瘤细胞表达了apoE〓重组蛋白，这种内源性的apoE〓重组蛋白对25μM Aβ25—35多肽诱导的神经成纤维胶质瘤细胞的死亡具有拮抗作用。

Conclusion］Magnesium alloys showed good biocompatibility to L929 mouse fibroblasts, and had no cacoethic effect to test animals, so the magnesium alloys is possible to be a new type and potential of bone surgery implant materials.

结论］镁合金对L929小鼠成纤维细胞有良好的生物相容性，对植入的动物也未见不良的影响，具有良好的生物安全性。因此可以初步认为镁合金作为一种新型的，有潜力的骨科内置物材料是可行的。

The cooperation of 8-MOP/UVA could induced dermis of the non- lesion back skin of vulgar psoriatic patients and the cultured dermic fibroblasts produced large aging-associated mutations of mtDNA rapidly, it is feasible to establish human models in vivo and in vitro which could produced large aging-assoiated mutations of mtDNA rapidly for studies of the mechanisms of mutations of mtDNA in skin photoaging.

2.8-MOP/UVA可诱导寻常型银屑病患者背部非皮损区真皮组织及培养的成纤维细胞迅速产生光老化相关的mtDNA突变，建立迅速产生光老化相关mtDNA突变的体内外模型，利用该模型进一步研究mtDNA突变在光老化皮肤中的产生机制及其在皮肤光老化形成中的作用是可行的。

Amino acid similarities between goat and human were 87% and between gaot and mouse were 83%. Just like other members of FGFs superfamily, FGF5 protein also has a signal sequence ,at same time its protein has higly similar sequence about 120 Amino acid in central core sequence with other breed. Tissue specific expression of the FGF5 gene was also investigated in cashmere goat by RT-PCR analysis. The results showed that FGF5 gene was expressed in different time of the tested skin tissues, including anage and telegon.

结果如下：(1)本研究利用 RT-PCR 方法克隆了羊 FGF5 基因的 cDNA 序列，得到了 925bp的序列，并对该序列进行了分析，与成纤维细胞生长因子家族的成员一样也是由三个外显子组成，也具有一信号序列，成熟蛋白为 270 个氨基酸，经同源性比对得出大鼠与小鼠的 FGF5 氨基酸同源性最高为 97%，在与羊的蛋白质比较中大鼠与羊的同源性为 83%，小鼠与羊的同源性为 84%，人与羊的同源性最高，为 89%。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

Some cell dropped into the cavity and became free. Thrombosis or part organization could be seen. The internal elastic layer became thin, disappear or broken. In internal and middle layer existed fibroblasts, fibrocytes and collagen. Some of the wall indicated hyaline change, soomth muscle cell decreased greatly. The massive inflammatory cells invaded the middle and external layer. There were many foam cells in the capsule tissue. Cytoplasm was filled with fatty tissue and cholesterol. some cavities were full of thrombosis. Some thrombosis was fibrosis, the bottom was organization. The surface of the thrombosis existed red blood cell and librae.(2)Elatic fibrila staining: the internal elastic menbrane almost completely disappeared, the intact internal elastic menbran could be seen in the new small vessels.

动脉瘤管壁厚薄明显不均，全层或局部区域显著变薄向外膨出，内皮细胞空泡变性或坏死脱落，部分内皮细胞剥离并突入管腔成游离状，可见血栓形成及部分血栓机化；内弹力板变薄、消失或突然中断；在内膜及中膜部位主要为纤维母细胞、纤维细胞和大片胶原；部分动脉瘤壁呈均质状玻璃样变，平滑肌细胞明显减少；中膜和外膜可见大量的炎性细胞浸润；瘤壁组织有纤维母细胞、纤维细胞、大片胶原成分及较多泡沫细胞，胞浆内充满脂类物质及胆固醇结晶；部分动脉瘤腔内充满血栓，有的血拴已经纤维化，血栓基部机化，血栓表面有红细胞和纤维素。

In contrast to rat-tail collagen-based HSEs, the present fibroblast-derived matrix-based HSEs contain more continuity in the number of viable cell layers in long-term cultures.

同鼠尾胶原来源的皮肤替代物相比较，在长期的培养过程中人成纤维来源的的模型包含更多的数量的细胞层。

Objective: To probe into the role of periostin in the formation of scars and investigate its reaction to hydrocortisone.

目的：通过检测periostin在人过度增生性瘢痕成纤维细胞中的表达及其对氢化可的松作用的反应，探讨periostin在瘢痕形成中的作用。

Objective: To observe production of antibody to rhbFGF, its neutralizing activity and deposition in kidney in conducting toxicity study on repeated doses of recombinant human basic fibroblast growth factor in normal rhesus monkeys and Wistar rats.

目的：在猕猴和大鼠中进行重组人碱性成纤维细胞生长因子的重复给药毒性试验时，观察给药后抗 rhbFGF 抗体的产生和抗体的中和性，以及抗体在肾脏的沉积。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。