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The recombinant fowl poxvirus rFPV-E0-E2 was serially passaged for 20 passages in specific-pathogen-free chicken embryo fibroblast culture. The passage 5, 10, 15 and 20 were chosen to identify by blue plaque assay, PCR amplification, gene sequence analysis and indirect immunofluoresence assay.

将重组鸡痘病毒rFPV-E0-E2在鸡胚成纤维细胞上连续传20代,取第5、10、15和20代重组病毒进行以下检测:用蓝斑试验鉴定各代重组病毒纯度;用PCR方法扩增猪瘟病毒E0、E2基因,进行基因序列分析;用间接免疫荧光实验检测各代重组病毒中猪瘟病毒E0、E2基因的表达。

"Second, both solid tumors and ascular anomalies (e.g., hemangiomas, port-wine stains) are known to hae increased expression of angiogenic proteins, including ascular endothelial growth factor and basic fibroblast growth factor."

&第二,已知实体瘤和脉管异常(例如血管瘤、葡萄酒色痣)存在血管原的蛋白表达增加,包括血管内皮生长因子和碱性成纤维细胞生长因子。&

OBJECTIVE: To observe the effect of acupotomology intervened third lumbar transverse process syndrome rat on levels of the fibroblast proliferation.

目的:观察针刀疗法对第三腰椎横突综合征模型大鼠组织中成纤维细胞增殖的影响。

OBJECTIVE: To observe the influence of nerve growth factor on the biological characteristics of scar fibroblasts.

目的:观察神经生长因子对瘢痕成纤维细胞生物学特征的影响。

Fibroblasts are responsible for the production of collagen.

成纤维细胞,负责生产的胶原蛋白。

Objective: To study the effects of platelet-rich plasma on the attachment and proliferation of dental pulp fibroblasts.

目的 :探讨富血小板血浆对牙髓成纤维细胞附着、增殖的影响。

Objective To study the effect of human insulin gene modified fibroblasts on blood glucose in diabetic rats.

目的 研究人胰岛素基因表达质粒转染鼠成纤维细胞后对糖尿病大鼠血糖的影响。

Objective To explore the method of isolation, cultivation and identification for human skin fibroblasts in vitro.

目的 探索和建立人皮肤成纤维细胞体外分离、培养及鉴定的方法。

Objective To discuss the effects of rhidosin on expression of fibroblast growth factor on olfactory bulb in senile rats and its significance.

目的 探讨红景天素对老龄大鼠嗅球中成纤维细胞生长因子(fibroblast growth factor, FGF)表达的影响及意义。

Objective To investigate the role of fibroblast growth factor-23 (FGF23) in secondary hyperparathyroidism.

目的 研究成纤维细胞生长因子23(FGF23)在继发性甲状旁腺功能亢进症中的作用。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

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关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。